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Aloe Vera Gel Extract Stimulates The Proliferation Of Primary Cultured Human Periodontal Ligament Cells And Pulpal Fibroblasts, Pasutha Thunyakitpisal, Kanoknadda Tavedhikul, Kulwadee Heamkulchon

Chulalongkorn University Dental Journal

Objective To study the effect of aloe vera gel extract and aloe vara exudates on the proliferation of primary cultured periodontal cells, pulpal fibroblasts, and gingival fibroblasts Materials and methods Cells were treated with the aloe vera gel extract, aloe vera exudate, and the lyophilized aloe vera gel extract in the serum-free media for 24 hours. The number of cells were measured by MTT technique and analyzed by One way ANOVA (α < 0.05). Results The aloe vera gel extract, at protein concentration 5, 10, 20 and 50 µg/ml, significantly increased the proliferation of periodontal cells and pulpal fibroblasts compared with the control group. The aloe vera gel exudates, at protein concentration 400 and 600 µg/ml, significantly decreased the proliferation of periodontal cells and pulpal fibroblasts as compared with the control group. The lyophilized aloe vera gel extract, at protein concentration 50, 100 and 200 µg/ml, significantly increased the proliferation of periodontal cells and pulpal fibroblasts. The lyophilized aloe vera gel extract, at protein concentration 50 and 100 µg/ml, significantly increased the proliferation of gingival fibroblasts compared with the control group. Conclusion The aloe vera gel extract (5-50 µg/ml) and lyophilized aloe vera gel extract (50-200 µg/ml) significantly induce the proliferation of periodontal cells and pulpal fibroblasts. The lyophilized aloe vera gel extract (50-100 µg/ml) significantly induce the proliferation of gingival fibroblasts. The aloe vera gel exudates (400-600 µg/ml) significantly decreased the cell number of periodontal cells and pulpal fibroblasts.