Dentistry Commons^™

Mechanism Of Mechanical Vibration In Enhancing Orthodontic Retention, Brent A. Ito

Master's Theses (2009 -)

Introduction: Low-magnitude, high-frequency (LMHF) mechanical vibration is proven to be anabolic and can increase alveolar bone mass. The objectives of this study were to generate an orthodontic retention cell culture model and investigate the effects of LMHF mechanical vibration on human periodontal ligament fibroblast (HPLF) cells after orthodontic loading (retention) in vitro. Materials and Methods: HPLF cells were seeded at a density of 4x105/well in 6-well Flexcell culture plates (Day 0). On day 3, the cells were mechanically stretched (15% surface extension) for 1 hour to mimic orthodontic loading/tension in vitro. On day 5, the cells were randomly assigned to …