Dentistry Commons^™

Use Of Micro-Ct To Examine Effects Of Heat On Coronal Obturation, Ivy Shen, Joan Daniel, Kali Vo, Chul Ahn, Carolyn Primus, Takashi Komabayashi

Dental Medicine Faculty Publications

Purpose: The aim of this study was to categorize the effects of heat on coronal obturation with gutta-percha and sealer using X-ray micro-computed tomography (micro-CT).
Methods: Ten single-rooted, extracted human teeth were shaped using ProTaper NEXT files to size X5 (#50/Taper 6%) with 2.5% NaOCl irrigation. A single ProTaper NEXT X5 gutta-percha point was then inserted with epoxy resin (AH Plus) or tricalcium silicate (EndoSequence BC) sealer (n = 5/group), and cut at the cemento-enamel junction. The teeth were scanned using micro-CT (SkyScan1272) to obtain 11 sagittal 2-D images. Three calibrated raters categorized the coronal 0.5 mm of the …

Coronal And Apical Leakage Among Five Endodontic Sealers, Kali Vo, Joan Daniel, Chul Anh, Carolyn Primus, Takashi Komabayashi

Dental Medicine Faculty Publications

Purpose: The aim of this study was to use dye penetration to measure apical and coronal leakage simultaneously in single-canal teeth that had been treated endodontically using a single-cone obturation technique.

Methods: One hundred single-canal, extracted human teeth were cleaned and shaped with ProTaper NEXT rotary files to size-X5 (50/.06), then randomly assigned to five sealer groups for single-cone gutta-percha obturation. The teeth were soaked in 0.6% rhodamine B at 37°C for seven days, then the roots were ground mesiodistally and the maximum apical and coronal dye penetration was measured. Differences in leakage among the sealer groups were examined using …

Effects Of Heat On Seven Endodontic Sealers, Daniel Chavarria-Bolanos, Takashi Komabayashi, Ivy Shen, Jose Vega-Baudrit, Maria G. Gandolfi, Carlo Prati, Mauricio Montero-Aguilar

Dental Medicine Faculty Publications

Purpose: To examine the microscopic surface features, chemical composition, and thermodynamic profile of seven endodontic sealers (AH Plus, Adseal, MTA-Fillapex, RoekoSeal, GuttaFlow 2, GuttaFlow BioSeal, and EndoRez) exposed to high-temperature changes using an endodontic obturation device.

Methods: The thermal properties were examined using scanning calorimetry (DSC) and thermogravimetric analysis (TGA). Then, six disc-shaped specimens of each sealer were prepared and divided into two groups – a room temperature group and a heat exposure group – for analysis of surface and chemical changes using scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS).

Results: DSC analysis showed that AH Plus had …

Dental Pulp Stem Cell Heterogeneity: Finding Superior Quality “Needles” In A Dental Pulpal “Haystack” For Regenerative Medicine-Based Applications, Zi Y. Kok, Nadia Y.A. Alaidaroos, Amr Alraies, John S. Colombo, Lindsay C. Davies, Rachel J. Waddington, Alastair J. Sloan, Ryan Moseley

Dental Medicine Faculty Publications

Human dental pulp stem/stromal cells (hDPSCs) derived from the permanent secondary dentition are recognised to possess certain advantageous traits, which support their potential use as a viable source of mesenchymal stem/stromal cells (MSCs) for regenerative medicine-based applications. However, the well-established heterogeneous nature of hDPSC subpopulations, coupled with their limited numbers within dental pulp tissues, has impeded our understanding of hDPSC biology and the translation of sufficient quantities of these cells from laboratory research, through successful therapy development and clinical applications. This article reviews our current understanding of hDPSC biology and the evidence underpinning the molecular basis of their heterogeneity, which …