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Full-Text Articles in Dentistry
Dental Pulp Stem Cell Heterogeneity: Finding Superior Quality “Needles” In A Dental Pulpal “Haystack” For Regenerative Medicine-Based Applications, Zi Y. Kok, Nadia Y.A. Alaidaroos, Amr Alraies, John S. Colombo, Lindsay C. Davies, Rachel J. Waddington, Alastair J. Sloan, Ryan Moseley
Dental Pulp Stem Cell Heterogeneity: Finding Superior Quality “Needles” In A Dental Pulpal “Haystack” For Regenerative Medicine-Based Applications, Zi Y. Kok, Nadia Y.A. Alaidaroos, Amr Alraies, John S. Colombo, Lindsay C. Davies, Rachel J. Waddington, Alastair J. Sloan, Ryan Moseley
Dental Medicine Faculty Publications
Human dental pulp stem/stromal cells (hDPSCs) derived from the permanent secondary dentition are recognised to possess certain advantageous traits, which support their potential use as a viable source of mesenchymal stem/stromal cells (MSCs) for regenerative medicine-based applications. However, the well-established heterogeneous nature of hDPSC subpopulations, coupled with their limited numbers within dental pulp tissues, has impeded our understanding of hDPSC biology and the translation of sufficient quantities of these cells from laboratory research, through successful therapy development and clinical applications. This article reviews our current understanding of hDPSC biology and the evidence underpinning the molecular basis of their heterogeneity, which …
Transcriptomic Phases Of Periodontitis Lesions Using The Nonhuman Primate Model, Jeffrey L. Ebersole, Radhakrishnan Nagarajan, Sreenatha Kirakodu, Octavio A. Gonzalez
Transcriptomic Phases Of Periodontitis Lesions Using The Nonhuman Primate Model, Jeffrey L. Ebersole, Radhakrishnan Nagarajan, Sreenatha Kirakodu, Octavio A. Gonzalez
Dental Medicine Faculty Publications
We used a nonhuman primate model of ligature-induced periodontitis to identify patterns of gingival transcriptomic after changes demarcating phases of periodontitis lesions (initiation, progression, resolution). A total of 18 adult Macaca mulatta (12–22 years) had ligatures placed (premolar, 1st molar teeth) in all 4 quadrants. Gingival tissue samples were obtained (baseline, 2 weeks, 1 and 3 months during periodontitis and at 5 months resolution). Gene expression was analyzed by microarray [Rhesus Gene 1.0 ST Array (Affymetrix)]. Compared to baseline, a large array of genes were significantly altered at initiation (n = 6049), early progression (n = 4893), and late progression …
Epidemiologic Evaluation Of Nhanes For Environmental Factors And Periodontal Disease, P. Emecen-Huja, H-F Li, Jeffrey L. Ebersole, J. Lambert, H. Bush
Epidemiologic Evaluation Of Nhanes For Environmental Factors And Periodontal Disease, P. Emecen-Huja, H-F Li, Jeffrey L. Ebersole, J. Lambert, H. Bush
Dental Medicine Faculty Publications
Periodontitis is a chronic inflammation that destroys periodontal tissues caused by the accumulation of bacterial biofilms that can be affected by environmental factors. This report describes an association study to evaluate the relationship of environmental factors to the expression of periodontitis using the National Health and Nutrition Examination Study (NHANES) from 1999–2004. A wide range of environmental variables (156) were assessed in patients categorized for periodontitis (n = 8884). Multiple statistical approaches were used to explore this dataset and identify environmental variable patterns that enhanced or lowered the prevalence of periodontitis. Our findings indicate an array of environmental variables were …
Serum Nutrient Levels And Aging Effects On Periodontitis, Jeffrey L. Ebersole, Joshua Lambert, Heather Bush, Pinar Emecen Huja, Arpita Basu
Serum Nutrient Levels And Aging Effects On Periodontitis, Jeffrey L. Ebersole, Joshua Lambert, Heather Bush, Pinar Emecen Huja, Arpita Basu
Dental Medicine Faculty Publications
Periodontal disease damages tissues as a result of dysregulated host responses against the chronic bacterial biofilm insult and approximately 50% of US adults >30 years old exhibit periodontitis. The association of five blood nutrients and periodontitis were evaluated due to our previous findings regarding a potential protective effect for these nutrients in periodontal disease derived from the US population sampled as part of the National Health and Nutrition Examination Survey (1999–2004). Data from over 15,000 subjects was analyzed for blood levels of cis-β-carotene, β-cryptoxanthin, folate, vitamin D, and vitamin E, linked with analysis of the presence and severity of periodontitis. …
Sustained Release Of Calcium Hydroxide From Poly(Dl-Lactide-Co-Glycolide) Acid Microspheres For Apexification, Bernardino Isaac Cerda-Cristerna, Alejandro Breceda-Leija, Verónica Méndez-González, Daniel Chavarría-Bolaños, Héctor Flores-Reyes, Arturo Garrocho-Rangel, Takashi Komabayashi, Aniket S. Wadajkar, Amaury J. Pozos-Guillén
Sustained Release Of Calcium Hydroxide From Poly(Dl-Lactide-Co-Glycolide) Acid Microspheres For Apexification, Bernardino Isaac Cerda-Cristerna, Alejandro Breceda-Leija, Verónica Méndez-González, Daniel Chavarría-Bolaños, Héctor Flores-Reyes, Arturo Garrocho-Rangel, Takashi Komabayashi, Aniket S. Wadajkar, Amaury J. Pozos-Guillén
Dental Medicine Faculty Publications
Calcium hydroxide (Ca(OH)2) loaded poly(DL-lactide-co-glycolide) acid (PLGA) microspheres (MS) might be employed for apexification requiring a sustained release of Ca++. The aim of this study was to formulate and characterize Ca(OH)2-PLGA-MS. The Ca(OH)2-loaded MS were prepared by either oil-in-water (O/W) or water-in-oil/in-water (W/O/W) emulsion solvent evaporation technique. MS produced by the O/W technique exhibited a larger diameter (18.63 ± 7.23 μm) than the MS produced by the W/O/W technique (15.25 ± 7.37 μm) (Mann Whitney U test P < 0.001). The Ca(OH)2 encapsulation efficiency and Ca++ release were calculated from data obtained by absorption techniques. Ca++ release profile was evaluated for 30 days. The percentage of encapsulation efficiency of the O/W-produced MS was higher (24%) than the corresponding percentage of the W/O/W-produced MS (11%). O/W- and W/O/W-produced MS released slower and lower Ca++ than a control Ca(OH)2 paste with polyethylene glycol 400 (ANOVA 1 way, Tukey HSD P < 0.01). O/W-produced MS released higher Ca++ than W/O/W-produced MS (statistically significant differences with t-Student test). We concluded that Ca(OH)2-PLGA-MS were successfully formulated; the technique of formulation influenced on the size, encapsulation efficiency and release profile. The MS were better sustained release system than the Ca(OH)2 paste.