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Full-Text Articles in Dentistry
Contact Point, Arthur A. Dugoni School Of Dentistry
Contact Point, Arthur A. Dugoni School Of Dentistry
Contact Point
No abstract provided.
Contact Point, Arthur A. Dugoni School Of Dentistry
Contact Point, Arthur A. Dugoni School Of Dentistry
Contact Point
No abstract provided.
Serum Decreases The Size Of Metafectene- And Genejammer-Dna Complexes But Does Not Affect Significantly Their Transfection Activity In Sccvii Murine Squamous Cell Carcinoma Cells, Krystyna Konopka, Nathan Overlid, Anitha C. Nagaraj, Nejat Düzgüneş
Serum Decreases The Size Of Metafectene- And Genejammer-Dna Complexes But Does Not Affect Significantly Their Transfection Activity In Sccvii Murine Squamous Cell Carcinoma Cells, Krystyna Konopka, Nathan Overlid, Anitha C. Nagaraj, Nejat Düzgüneş
All Dugoni School of Dentistry Faculty Articles
Cationic liposome-DNA (lipoplexes) or polymer-DNA (polyplexes) complexes have been used to deliver therapeutic genes, both in vitro and in vivo. However, gene transfer by these non-viral vectors is usually inhibited by biological milieu. A relatively high efficiency of transfection could be achieved in human oral cancer cells transfected with the polycationic liposome, Metafectene, and the polyamine reagent, GeneJammer, in the presence of 60% fetal bovine serum (FBS) (Konopka et al., Cell. Mol. Biol. Lett. 10 (2005) 455-470). Here, we examined the efficacy of these vectors to deliver β-galactosidase (β-gal), luciferase and Herpes Simplex Virus thymidine kinase (HSV-tk) genes to SCCVII …