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Full-Text Articles in Dentistry
The Shape Parameter Of Liposomes And Dna-Lipid Complexes Determined By Viscometry Utilizing Small Sample Volumes, Yan Sun, X. Li, Nejat Düzgüneş, Y. Takaoka, S. Ohi, S. Hirota
The Shape Parameter Of Liposomes And Dna-Lipid Complexes Determined By Viscometry Utilizing Small Sample Volumes, Yan Sun, X. Li, Nejat Düzgüneş, Y. Takaoka, S. Ohi, S. Hirota
All Dugoni School of Dentistry Faculty Articles
A minicapillary viscometer utilizing <0.5 ml of sample at a volume fraction of <0.1% is described. The calculated a/b of DPPC/DPPG multilamellar liposome was 1.14 as prolate ellipsoids and a/b of dioleoylpropyltrimethyl ammonium methylsulfate-DNA complex at a charge ratio of 4:1 (+/-) was 3.7 as prolate ellipsoids or 4.9 as oblate ellipsoids. The deviation of shape from perfect sphere is thus expressed quantitatively in more than two significant figures. In these measurement, the necessary amount of DNA is <0.5 mg.
5th Annual Pacific Research Day Abstracts, University Of The Pacific
5th Annual Pacific Research Day Abstracts, University Of The Pacific
Excellence Day
No abstract provided.
Increased Receptor-Mediated Gene Delivery To The Liver By Protamine-Enhanced-Asialofetuin-Lipoplexes, M. A. Arangoa, Nejat Düzgüneş, C. Tros De Ilarduya
Increased Receptor-Mediated Gene Delivery To The Liver By Protamine-Enhanced-Asialofetuin-Lipoplexes, M. A. Arangoa, Nejat Düzgüneş, C. Tros De Ilarduya
All Dugoni School of Dentistry Faculty Articles
A novel lipidic vector composed of DOTAP/Chol liposomes, asialofetuin (AF), protamine sulfate and DNA has been developed. The resulting protamine-AF-lipoplexes improved significantly the levels of gene expression in cultured cells and in the liver upon i.v. administration. Lipoplexes containing the optimal amount of AF (1 μg/μg DNA) showed a 16-fold higher transfection activity in HepG2 cells than non-targeted (plain) complexes. The uptake by cells having asialoglycoprotein receptors (ASGPr) on their plasma membrane was decreased by the addition of free AF, indicating that AF-lipoplexes were taken up specifically by cells via ASGPr-mediated endocytosis. Results from transfections performed in cells defective in …