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Full-Text Articles in Dentistry
Human Immunodeficiency Virus Type-1 (Hiv-1) Infection Increases The Sensitivity Of Macrophages And Thp-1 Cells To Cytotoxicity By Cationic Liposomes, Krystyna Konopka, Elizabeth Pretzer, Philip L. Felgner, Nejat Düzgüneş
Human Immunodeficiency Virus Type-1 (Hiv-1) Infection Increases The Sensitivity Of Macrophages And Thp-1 Cells To Cytotoxicity By Cationic Liposomes, Krystyna Konopka, Elizabeth Pretzer, Philip L. Felgner, Nejat Düzgüneş
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Cationic liposomes may be valuable for the delivery of anti-sense oligonucleotides, ribozymes, and therapeutic genes into human immunodeficiency virus type 1 (HIV-1)-infected and uninfected cells. We evaluated the toxicity of three cationic liposomal preparations, Lipofectamine, Lipofectin, and 1,2-dimyristyloxypropyl-3-dimethyl-hydroxyethyl ammonium bromide (DMRIE) reagent, to HIV-infected and uninfected cells. Monocyte/macrophages were infected with HTV-1(BaL) and treated with liposomes in medium containing 20% fetal bovine serum (FBS) for 4 h or 24 h at 37°C. Uninfected monocytic THP-1 cells and chronically infected THP-1/HIV-1(IIIB) cells were treated with phorbol 12-myristate 13-acetate (PMA) and exposed to liposomes in the presence of 10% FBS. Toxicity was …
Treatment Of Intracellular Mycobacterium Avium Complex Infection By Free And Liposome-Encapsulated Sparfloxacin, Nejat Düzgüneş, Diana L. Flasher, M. Venkata Reddy, Julieta Luna-Herrera, Pattisapu R.J. Gangadharam
Treatment Of Intracellular Mycobacterium Avium Complex Infection By Free And Liposome-Encapsulated Sparfloxacin, Nejat Düzgüneş, Diana L. Flasher, M. Venkata Reddy, Julieta Luna-Herrera, Pattisapu R.J. Gangadharam
All Dugoni School of Dentistry Faculty Articles
Mycobacterium avium-M. intracellulare complex (MAC) is the most frequent cause of opportunistic bacterial infection in patients with AIDS. Previous studies have indicated that liposome-encapsulated aminoglycosides are highly effective in treating MAC infections in mice. We investigated whether the fluoroquinolone sparfloxacin is effective in treating MAC infection in the murine macrophage-like cell line J774. Sparfloxacin was encapsulated in the membrane phase of multilamellar liposomes composed of phosphatidylglycerol-phosphatidylcholine-cholesterol (1:1:1 molar ratio). MAC-infected macrophages were treated for either 24 h or 4 days with free or liposome-encapsulated sparfloxacin. Treatment with free or liposome- encapsulated sparfloxacin (6 μg/ml) for 24 h resulted in the …