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Full-Text Articles in Dentistry
Target Cell Membrane Sialic Acid Modulates Both Binding And Fusion Activity Of Influenza Virus, Maria C. Pedroso De Lima, João Ramalho-Santos, Diana L. Flasher, Vladimir A. Slepushkin, Shlomo Nir, Nejat Düzgüneş
Target Cell Membrane Sialic Acid Modulates Both Binding And Fusion Activity Of Influenza Virus, Maria C. Pedroso De Lima, João Ramalho-Santos, Diana L. Flasher, Vladimir A. Slepushkin, Shlomo Nir, Nejat Düzgüneş
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Influenza virus binds to cell surface sialic acid receptors, and following endocytosis fuses with the endosome membrane at low pH. Whether sialic acid plays a role in the virus-cell membrane fusion step is not known. We investigated the effect of the removal of cell membrane sialic acid on the fusion activity of influenza virus (A/PR/8/34 strain) toward human T lymphocytic leukemia (CEM) cells at low pH. Fusion was monitored by fluorescence dequenching of octadecylrhodamine incorporated in the virus membrane. Removal of sialic acid by neuraminidase resulted in a drastic reduction in both viral binding and fusion. The association of the …
Therapy Of Mycobacterium Avium Complex Infections In Beige Mice With Streptomycin Encapsulated In Sterically Stabilized Liposomes, Pattisapu R.J. Gangadharam, D. R. Ashtekar, Diana L. Flasher, Nejat Düzgüneş
Therapy Of Mycobacterium Avium Complex Infections In Beige Mice With Streptomycin Encapsulated In Sterically Stabilized Liposomes, Pattisapu R.J. Gangadharam, D. R. Ashtekar, Diana L. Flasher, Nejat Düzgüneş
All Dugoni School of Dentistry Faculty Articles
Mycobacterium avium complex (MAC) causes serious opportunistic infections in AIDS patients. Previous studies with MAC-infected beige mice have indicated that weekly administration of liposome-encapsulated streptomycin can reduce significantly the CFU in the liver and spleen. We examined whether streptomycin encapsulated in recently developed sterically stabilized liposomes with prolonged circulation times would have a therapeutic effect in this animal model. Two liposome types with prolonged circulation (polyethyleneglycol-distearoylphosphatidylethanolamine [PEG-DSPE]- distearoylphosphatidylcholine [DSPC]-cholesterol [chol] or phosphatidylinositol [PI]-DSPC-chol) and conventional liposomes (phosphatidylglycerol [PG]-phosphatidylcholine [PC]-chol) encapsulating streptomycin and administered twice weekly were bactericidal to MAC strain 101 in the spleen when the level of infection after …
A Monoclonal Antibody To The Gp120-Cd4 Complex Has Differential Effects On Hiv-Induced Syncytium Formation And Viral Infectivity, Krystyna Konopka, Elizabeth Pretzer, F. Celada, Nejat Düzgüneş
A Monoclonal Antibody To The Gp120-Cd4 Complex Has Differential Effects On Hiv-Induced Syncytium Formation And Viral Infectivity, Krystyna Konopka, Elizabeth Pretzer, F. Celada, Nejat Düzgüneş
All Dugoni School of Dentistry Faculty Articles
A murine monoclonal antibody (MAb F-91-55) raised against the complex of soluble CD4 and human immunodeficiency virus type 1 (HIV-1) gp120 had previously been found to inhibit syncytium formation without inhibiting the interaction of CD4 with gp120, and its binding site was localized within the first two domains (D1/D2) of CD4. We investigated whether this antibody inhibited the infectivity of HIV-1 in the CD4+ T cell lines A3.01, Sup-T1 and H9. We also examined the effect of the antibody on syncytium formation between these cells and chronically infected H9 cells. Syncytium formation was found to depend critically on the incubation …