Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Dentistry
A Common Mechanism For Influenza Virus Fusion Activity And Inactivation, João Ramalho-Santos, Shlomo Nir, Nejat Düzgüneş, Arsélio Pato De Carvalho, Maria Conceição Pedroso De Lima
A Common Mechanism For Influenza Virus Fusion Activity And Inactivation, João Ramalho-Santos, Shlomo Nir, Nejat Düzgüneş, Arsélio Pato De Carvalho, Maria Conceição Pedroso De Lima
All Dugoni School of Dentistry Faculty Articles
The fusion of influenza virus (A/PR/8/34 strain) with PC-12 cells was monitored by a fluorescence assay, and the results were analyzed with a mass-action model which could explain and predict the kinetics of fusion. The model accounted explicitly for the reduction in the fusion rate constant upon exposure of the virus to low pH, either for the virus alone in suspension or for the virus bound to the cells. When the pH was lowered without previous viral attachment to cells, an optimal fusion activity was detected at pH 5.2. When the virus was prebound to the cells, however, reduction of …
Anionic Liposomes Inhibit Human Immunodeficiency Virus Type 1 (Hiv-1) Infectivity In Cd4+ A3.01 And H9 Cells, Krystyna Konopka, B. R. Davis, C. E. Larsen, Nejat Düzgüneş
Anionic Liposomes Inhibit Human Immunodeficiency Virus Type 1 (Hiv-1) Infectivity In Cd4+ A3.01 And H9 Cells, Krystyna Konopka, B. R. Davis, C. E. Larsen, Nejat Düzgüneş
All Dugoni School of Dentistry Faculty Articles
Immunodeficiency viruses undergo fusion with liposomes containing anionic phospholipids (Larsen et al., 1990). We have investigated the effect of liposomes composed of cardiolipin, phosphatidylserine or phosphatidylinositol, on the infectivity of three strains of HIV-1 in A3.01 and H9 cells, measured by p24 (gag) production in the medium. The infectivity of HIV-1 in A3.01 or H9 cells was inhibited by the presence of cardiolipin liposomes during a 2 h infection period, with IC50's of 23.0, 4.8, and 5.0 μM phospholipid, respectively, for the different strains. Liposomes composed of phosphatidylserine or phosphatidylinositol were ineffective under similar conditions. However, prolonged pre-incubation of the …
Simian Immunodeficiency Virus (Siv(Mac)251) Membrane Lipid Mixing With Human Cd4+ And Cd4- Cell Lines In Vitro Does Not Necessarily Result In Internalization Of The Viral Core Proteins And Productive Infection, L. Stamatatos, Nejat Düzgüneş
Simian Immunodeficiency Virus (Siv(Mac)251) Membrane Lipid Mixing With Human Cd4+ And Cd4- Cell Lines In Vitro Does Not Necessarily Result In Internalization Of The Viral Core Proteins And Productive Infection, L. Stamatatos, Nejat Düzgüneş
All Dugoni School of Dentistry Faculty Articles
The cell binding site of simian immunodeficiency virus (SIV) is believed to be the CD4 molecule. Several CD4+ cell lines are, however, resistant to infection by SIV(mac)251 in vitro and additional cell membrane molecules have been implicated in SIV(mac)251 entry. We investigated the binding, envelope fusion and entry of the viral core proteins (p27) of SIV(mac)251 into two human CD4+ cell lines (H9 and Sup-T1) which are infectible, and one CD4+ (A3.01) and two CD4- cell lines (K562 and Raji) that are resistant to infection. The fusion of the viral and cellular membranes was monitored by a fluorescence assay for …