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Full-Text Articles in Dentistry
Liposomes Modulate Human Immunodeficiency Virus Infectivity, Krystyna Konopka, B. R. Davis, C. E. Larsen, D. R. Alford, Robert J. Debs, Nejat Düzgüneş
Liposomes Modulate Human Immunodeficiency Virus Infectivity, Krystyna Konopka, B. R. Davis, C. E. Larsen, D. R. Alford, Robert J. Debs, Nejat Düzgüneş
All Dugoni School of Dentistry Faculty Articles
We have investigated the effects of the fusion of liposomes with human immunodeficiency virus type 1 (HIV-1(LVA)) on the ability of the virus to infect CD4+ and CD4- cells. Fluorescence dequenching measurements indicated that HIV-1 fuses with liposomes composed of either cardiolipin (CL) or N-[2,3-(dioleyloxy) propyl]-N,N,N-trimethyl ammonium chloride (DOTMA) but not appreciably with dioleoylphosphatidylcholine (DOPC) liposomes. Pre-incubation of HIV-1 with DOTMA liposomes enhanced virus production (measured by p24 gag antigen production in the culture medium and in situ) in CD4+ A3.01 and H9 cells in a concentration-dependent manner, but did not mediate the infection of the CD4- cell line, K562. …
Fusion Of Simian Immunodeficiency Virus With Liposomes And Erythrocyte Ghost Membranes: Effects Of Lipid Composition, Ph And Calcium, Charles E. Larsen, D. R. Alford, L. J.T. Young, T. P. Mcgraw, Nejat Düzgüneş
Fusion Of Simian Immunodeficiency Virus With Liposomes And Erythrocyte Ghost Membranes: Effects Of Lipid Composition, Ph And Calcium, Charles E. Larsen, D. R. Alford, L. J.T. Young, T. P. Mcgraw, Nejat Düzgüneş
All Dugoni School of Dentistry Faculty Articles
Simian immunodeficiency virus from macaques (SIV(mac)) is closely related in its structure and biological activity to human immunodeficiency virus, and is the best animal model for the acquired immunodeficiency syndrome. We investigated the kinetics of membrane fusion between SIV(mac) and phospholipid vesicles and the effects of various parameters on this process. Purified SIV(mac) was labelled with octadecyl rhodamine B chloride, and fusion was continuously monitored as the dilution of the probe in target membranes. These studies show that SIV(mac) fusion is strongly dependent upon the liposome composition. Fusion with pure cardiolipin (CL) liposomes is significantly faster than with CL/dioleoylphosphatidylcholine (DOPC) …