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Enhanced Effect Of Liposome-Encapsulated Amikacin On Mycobacterium Avium-M. Intracellulare Complex Infection In Beige Mice, Nejat Düzgüneş, Veluchamy K. Perumal, L. Kesavalu, Jayne A. Goldstein, Robert J. Debs, Pattisapu R.J. Gangadharam
Enhanced Effect Of Liposome-Encapsulated Amikacin On Mycobacterium Avium-M. Intracellulare Complex Infection In Beige Mice, Nejat Düzgüneş, Veluchamy K. Perumal, L. Kesavalu, Jayne A. Goldstein, Robert J. Debs, Pattisapu R.J. Gangadharam
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We examined the therapeutic effects of free and liposome-encapsulated amikacin on Mycobacterium avium-M. intracellulare complex infection by using the beige-mouse model of the disease. In the first series of studies, intravenous administration of four weekly doses of 5 mg of amikacin per kg encapsulated in large (approximately 0.4-μm diameter), unilamellar liposomes arrested the growth of M. avium-M. intracellulare complex organisms in the liver, as measured by CFU counts. M. avium-M. intracellulare complex levels in untreated animals and in those treated with the same dose of free amikacin increased by several orders of magnitude over 8 weeks. Liposome-encapsulated amikacin was also …
La3+-Induced Fusion Of Phosphatidylserine Liposomes. Close Approach, Intermembrane Intermediates, And The Electrostatic Surface Potential, Joe Bentz, D. R. Alford, J. Cohen, Nejat Düzgüneş
La3+-Induced Fusion Of Phosphatidylserine Liposomes. Close Approach, Intermembrane Intermediates, And The Electrostatic Surface Potential, Joe Bentz, D. R. Alford, J. Cohen, Nejat Düzgüneş
All Dugoni School of Dentistry Faculty Articles
The fusion of large unilamellar phosphatidylserine liposomes (PS LUV) induced by La3+ has been monitored using the 1-aminoapthalene-3,6,8-trisulfonic acid/p-xylenebis(pyridinium bromide) (ANTS/DPX) fluorescence assay for the mixing of aqueous contents. The fusion event is extensive and nonleaky, with up to 95% mixing of contents in the fused liposomes. However, addition of excess EDTA leads to disruption of the fusion products in a way that implies the existence of metastable intermembrane contact sites. The maximal fusion activity occurs between 10 and 100 microM La3+ and fusion can be terminated rapidly, without loss of contents, by the addition of excess La3+, e.g., 1 …