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Full-Text Articles in Dentistry
Oral Microbiome And Gingival Gene Expression Of Inflammatory Biomolecules With Aging And Periodontitis, Jeffrey L. Ebersole, Radhakrishnan Nagarajan, Sreenatha S. Kirakodu, Octavio A. Gonzalez
Oral Microbiome And Gingival Gene Expression Of Inflammatory Biomolecules With Aging And Periodontitis, Jeffrey L. Ebersole, Radhakrishnan Nagarajan, Sreenatha S. Kirakodu, Octavio A. Gonzalez
Center for Oral Health Research Faculty Publications
Although data describe the presence and increase of inflammatory mediators in the local environment in periodontitis vs. health in humans, details regarding how these responses evolve in the transition from health to disease, changes during disease progression, and features of a resolved lesion remain unknown. This study used a nonhuman primate model of ligature-induced periodontitis in young, adolescent, adult, and aged animals to document features of inflammatory response affected by age. Rhesus monkeys had ligatures tied and provided gingival tissue biopsy specimens at baseline, 0.5, 1, and 3 months of disease and at 5 months of the study, which was …
Reprogramming Oral Epithelial Keratinocytes Into A Pluripotent Phenotype For Tissue Regeneration, Fayrouz Bazina, Sabine M. Brouxhon, Stephanos Kyrkanides
Reprogramming Oral Epithelial Keratinocytes Into A Pluripotent Phenotype For Tissue Regeneration, Fayrouz Bazina, Sabine M. Brouxhon, Stephanos Kyrkanides
Center for Oral Health Research Faculty Publications
OBJECTIVES: We set out to reprogram adult somatic oral epithelial keratinocytes into pluripotent cells for regenerative dentistry.
SETTING AND SAMPLE POPULATION: Immortalized murine oral keratinocyte cell (IMOK) line raised from adult mouse mucosa were cultured in vitro in our studies.
MATERIALS AND METHODS: Adult murine oral epithelial keratinocytes were chronically treated with TGF-β1 in vitro, and the expression of Oct4, Nanog, Sox2 and Nestin, as well as specific homeobox Gata and Pax gene family members were investigated.
RESULTS: We documented the induction of stem factors linked with pluripotency and/or the maintenance and regulation of stem-cell self-renewal in oral epithelial keratinocytes …
Transcriptomic Phases Of Periodontitis Lesions Using The Nonhuman Primate Model, Jeffrey L. Ebersole, Radhakrishnan Nagarajan, Sreenatha S. Kirakodu, Octavio A. Gonzalez
Transcriptomic Phases Of Periodontitis Lesions Using The Nonhuman Primate Model, Jeffrey L. Ebersole, Radhakrishnan Nagarajan, Sreenatha S. Kirakodu, Octavio A. Gonzalez
Center for Oral Health Research Faculty Publications
We used a nonhuman primate model of ligature-induced periodontitis to identify patterns of gingival transcriptomic after changes demarcating phases of periodontitis lesions (initiation, progression, resolution). A total of 18 adult Macaca mulatta (12–22 years) had ligatures placed (premolar, 1st molar teeth) in all 4 quadrants. Gingival tissue samples were obtained (baseline, 2 weeks, 1 and 3 months during periodontitis and at 5 months resolution). Gene expression was analyzed by microarray [Rhesus Gene 1.0 ST Array (Affymetrix)]. Compared to baseline, a large array of genes were significantly altered at initiation (n = 6049), early progression (n = 4893), and late progression …