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Survival And Adherence Of Apical Stem Cells To Root Canal Dentin After Conditioning With Apple Vinegar, Hebatullah Safwat, Wael Kamel, Mohamed Kataia, Dina Sabry
Survival And Adherence Of Apical Stem Cells To Root Canal Dentin After Conditioning With Apple Vinegar, Hebatullah Safwat, Wael Kamel, Mohamed Kataia, Dina Sabry
Al-Azhar Journal of Dentistry
Purpose: To evaluate the survival and adherence of apical stem cells to root canal dentin after conditioning with apple vinegar. Materials and Methods: Stem cells from apical papilla of immature third molars were isolated. Survival of cells was evaluated by MTT test. Adherence of cells was evaluated by level of fibronectin expression and scanning electron microscope (SEM) observation. Thirty dentin discs from premolar teeth, extracted for orthodontic purposes, were immersed in 1.5% NaOCl then washed by PBS and finally divided into five groups regarding the final irrigating solution and time of its application. Group I: EDTA for 5 minutes, Group …