Dentistry Commons^™

Upregulated Mir-146a Expression In Peripheral Blood Mononuclear Cells From Rheumatoid Arthritis Patients, Kaleb M. Pauley, M. Satoh, A. L. Chan, M. R. Bubb, W. H. Reeves, E. K. Chan

Science and Mathematics Faculty Publications

Introduction

MicroRNAs are small noncoding RNA molecules that negatively regulate gene expression via degradation or translational repression of their targeted mRNAs. It is known that aberrant microRNA expression can play important roles in cancer, but the role of microRNAs in autoimmune diseases is only beginning to emerge. In this study, the expression of selected microRNAs is examined in rheumatoid arthritis.

Methods

Total RNA was isolated from peripheral blood mononuclear cells obtained from patients with rheumatoid arthritis, and healthy and disease control individuals, and the expression of miR-146a, miR-155, miR-132, miR-16, and microRNA let-7a was analyzed using quantitative real-time PCR.

Results …

Optimizing Qpcr For The Quantification Of Periodontal Pathogens In A Complex Plaque Biofilm, Sreenatha S. Kirakodu, M. Govindaswami, Michael John Novak, Jeffrey L. Ebersole, Karen F. Novak

Center for Oral Health Research Faculty Publications

Quantitative PCR (qPCR) has recently been used to quantify microorganisms in complex communities, including dental plaque biofilms. However, there is variability in the qPCR protocols being used. This study was designed to evaluate the validity of two of these variables with the intent of developing a more standardized qPCR protocol. The two variables evaluated were (1) the use of DNA content versus actual cell counts to estimate bacterial numbers in mixed plaque samples and (2) the effectiveness of three different universal primers versus species specific primers in amplifying specific target pathogens in these samples. Results lead to the development of …

Management Of Oral Mucositis In Patients With Cancer, Rajesh V. Lalla, Douglas E. Peterson

UCHC Articles - Research

Oral mucositis refers to erythematous and ulcerative lesions of the oral mucosa observed in patients with cancer being treated with chemotherapy, and/or with radiation therapy to fields involving the oral cavity. Lesions of oral mucositis are often very painful and compromise nutrition and oral hygiene as well as increase risk for local and systemic infection. Mucositis can also involve other areas of the alimentary tract; for example, gastrointestinal (GI) mucositis can manifest as diarrhea. Thus, mucositis is a highly significant and sometimes dose-limiting complication of cancer therapy.

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Modification Of Pmma Dental Resin With Palm Oil Based Polyol, Noor Hayaty Abu Kasim Abu Kasim N.H.

Noor Hayaty Abu Kasim Abu Kasim N.H.

Objectives: This study is an attempt to enhance the properties of conventional PMMA denture material by incorporation of a polyfunctional polyol derived from palm oil by copolymerization with methyl methacrylate monomer to overcome the low fatigue resistance property of PMMA. Methods: A series of copolymerizations of palm oil polyol with MMA was performed in solution. The polyol was added from 40-20 %, MMA from 20-40% , benzoyl peroxide as initiator was at 0.04% and toluene used as solvent was at 60 % of the total weight. The copolymerization was carried out in a 500ml-round-bottom reaction flask, equipped with a magnetic …

Conversion Of A New Monomer For Dental Resin Application, Noor Hayaty Abu Kasim Abu Kasim N.H.

Noor Hayaty Abu Kasim Abu Kasim N.H.

Objective: The aim of this study was to investigate the degree of conversion and cross-linking of polyurethane dimethacrylate (PUDMA), derived from palm oil polyol, for dental resin application. Methods: Polyurethane dimethacrylate was synthesized by reacting excess amount of Methylene diphenyl diisocyanate (MDI) with palm oil polyol. The resulting isocyanate polyurethane prepolymer was then acrylated using hydroxymethacrylate (HEMA). Fourier transform infrared spectroscopy was used to ascertain the formation of PUDMA, and to determine the degree of conversion (DC) of: I) PUDMA, II) Bis-GMA, III)Bis-EMA,IV) Bis-GMA/TEGDMA (3:1), V) Bis-GMA/TEGDMA(3:1)/Bis-EMA (3:1), VI) PUDMA/Bis-EMA (3:1), VII) Bis-GMA/TEGDMA(3:1)/PUDMA (1:1), and VIII) Bis-GMA/TEGDMA(3:1)/PUDMA/Bis-EMA (1-1-1). The photo …

Flexural Strength Of Pmma Modified With Palm-Oil Based Polyol, Noor Hayaty Abu Kasim Abu Kasim N.H.

Noor Hayaty Abu Kasim Abu Kasim N.H.

Objectives:Biocompatibility is an important desirable property for a dental polymer and it can be achieved by utilizing more materials from natural sources. The flexural strength of an experimental polymer prepared from MMA monomer modified with palm-oil polyol was compared to two commercial resins,namely the Lucitone 199® (Dentsply Trubyte, USA) and Impact (Ivoclar Vivadent, Liechtenstein). Methods: The experimental polymer was prepared by copolymerizing MMA monomer and palm oil polyol. The resulting polymer was cast into a plate form and sectioned using a precision diamond saw (Buhler, Germany) to form 10 specimens of dimension, 65mm x 11mm x 5mm. These specimens were …

Demineralization Potential Of Qat Extracts At Composite Restoration Interface, Noor Hayaty Abu Kasim Abu Kasim N.H.

Noor Hayaty Abu Kasim Abu Kasim N.H.

Objective: To evaluate the demineralization potential of qat extracts at composite restoration interface. Methods: Class V cavities were prepared on the buccal surface of thirty extracted sound premolars and were restored with a nanohybrid composite (Grandio, VOCO, Germany). Specimens were subsequently coated with nail varnish exposing 2mm of enamel around the restoration margin. The specimens were divided into three equal groups of 10 and were then immersed in acid gel and qat extracts (10% and 20%) for 4 weeks. All specimens were removed and washed using deionized water and later examined by direct vision and stereomicroscope prior to sectioning. Specimens …

Finite Element Analysis Of An Automated Rapid Maxillary Expander (Arme), Noor Hayaty Abu Kasim Abu Kasim N.H.

Noor Hayaty Abu Kasim Abu Kasim N.H.

An Automated Rapid Maxillary Expander (ARME), is a specially designed orthodontic appliance to overcome the shortcomings imposed by the traditional butterfly expansion appliance. It operates by automatically widening the maxilla (upper jaw) by expanding the midpalatal suture [1]. This procedure is not feasible after late teenage years due to more rigid facial skeleton features. According to the study by Cozzani [2], the activation of the device could produce 0.25 mm of expansion per day. Over the period of 2 weeks, the expansion produced is up to 7 mm in total [1]. For this study, we explored the mechanism involved in …

Acquiring Anatomical Representation Of Human Maxilla For Rapid Maxillary Expansion, Noor Hayaty Abu Kasim Abu Kasim N.H.

Noor Hayaty Abu Kasim Abu Kasim N.H.

The purpose of the study is to model and provide a better understanding of maxilla bone involved in the treatment for rapid maxillary expander (RME) for dento-facial applications. The treatment is recommended for patients presented with an arch width deficiency named cross bites. Cross bites often cause abnormal physical forces that disrupt the balance of the occlusal relationship. More commonly, the maxilla or the upper jaw appears to be narrow and contributes to significant degree of crowding in the mouth. Early investigators [1-4] discovered that rapid maxillary expansion resulted in a splitting of the median palatal suture. The expansion is …

The Effect Of Light Curing Tip Angulations On The Shear Bond Strength Of An Orthodontic Adhesive, Noor Hayaty Abu Kasim Abu Kasim N.H.

Noor Hayaty Abu Kasim Abu Kasim N.H.

The purpose of this study was to investigate the effect of light curing tip angulations on the shear bond strength of an orthodontic adhesive. Three groups of thirty premolar teeth had brackets attached in a standardized manner using Transbond XT. The orthodontic adhesive were cured with a Blue LED light curing unit for 40 seconds using three different angulations 0 degrees, 45 degrees and 9 degrees at a standardized 3 mm distance. The debonding force was measured using an Instron Universal Testing Machine. Data were computed and analysed using SPSS version 12.0. Kruskal-Wallis test was employed to compare the shear …

Validation Of Cone Beam Ct Scan For Measurement Of Palatal Depth In Study Casts, Noor Hayaty Abu Kasim Abu Kasim N.H.

Noor Hayaty Abu Kasim Abu Kasim N.H.

The purpose of this study was to validate the use of digital dental study casts obtained from Cone beam CT Scan (CBCT) against gold standard; that is traditional dental study cast measured with digital caliper. Thirty-four study casts of Malaysian Aborigines were selected from Centre of Malaysian Pribumi Studies Databank. Palatal depth of each study casts were measured using Mitutoyo digital caliper. Subsequently, all the study models were scanned using i-CAT CBCT and the palatal depth were measured using i-CAT software. Data were analysed using SPSS version 12.0 where Intraclass correlation and paired T-test were employed. There was no significant …

Cutting Efficiency Of Dental Burs-A Pilot Study, Noor Hayaty Abu Kasim Abu Kasim N.H.

Noor Hayaty Abu Kasim Abu Kasim N.H.

Objectives: This study aim to evaluate the cutting efficiency of tungsten carbide burs (TC) and rotary diamond instruments by measuring their rake angle and visual examination of their cutting surfaces respectively, using scanning electron microscopy (SEM). Materials and Method: 60 short and long head pear-shaped TC and 18 round rotary diamond instruments that had been used to prepare < than 5, > than 5 and > than 10 cavities were selected from the 3rd & 4th year dental students, Dental Faculty, University of Malaya. 12 cylindrical and flame rotary diamond instruments were also selected from the 5th year students. The rake angle of TC was …

Push-Out Bond Strength Of Luting Agents To Fibre-Reinforced Post: An In Vitro Study, Noor Hayaty Abu Kasim Abu Kasim N.H.

Noor Hayaty Abu Kasim Abu Kasim N.H.

The objective of this study was to investigate the effect of various luting agents on bond strength of fibre-reinforced posts to root canal dentine. 40 extracted single rooted sound premolar teeth were root filled, decoronated and randomly divided into four groups. Fibre posts, Aestheti-Plus (TM) (Bisco,Inc. Schaumburg, IL, USA) were cemented using four luting agents: Group A (control): Elite 1000 Zinc phosphate (GC Corp, Japan), Group B: Calibra (TM) Esthetic Resin Cement (Dentsply Caulk, USA), Group C: RelyX ARC Adhesive Resin (3M ESPE), Group D: RelyX Unicem Aplicap (3M ESPE). Each roots were sliced into 2 discs representing the coronal …