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Full-Text Articles in Medicine and Health Sciences
Transmembrane Domain Nrg1 Mutant Mice Show Altered Susceptibility To The Neurobehavioural Actions Of Repeated Thc Exposure In Adolescence, Leonora E. Long, Rose Chesworth, Xu-Feng Huang, Iain S. Mcgregor, Jonathon C. Arnold, Tim Karl
Transmembrane Domain Nrg1 Mutant Mice Show Altered Susceptibility To The Neurobehavioural Actions Of Repeated Thc Exposure In Adolescence, Leonora E. Long, Rose Chesworth, Xu-Feng Huang, Iain S. Mcgregor, Jonathon C. Arnold, Tim Karl
Faculty of Health and Behavioural Sciences - Papers (Archive)
Heavy cannabis abuse increases the risk of developing schizophrenia. Adolescents appear particularly vulnerable to the development of psychosis-like symptoms after cannabis use. To test whether the schizophrenia candidate gene neuregulin 1 (NRG1) modulates the effects of cannabinoids in adolescence, we tested male adolescent heterozygous transmembrane domain Nrg1 mutant (Nrg1 TM HET) mice and wild type-like littermates (WT) for their neurobehavioural response to repeated Δ9-tetrahydrocannabinol (THC, 10 mg/kg i.p. for 21 d starting on post-natal day 31). During treatment and 48 h after treatment withdrawal, we assessed several behavioural parameters relevant to schizophrenia. After behavioural testing we measured autoradiographic …
Teasaponin Reduces Inflammation And Central Leptin Resistance In Diet-Induced Obese Male Mice, Yinghua Yu, Yizhen Wu, Alexander Szabo, Zhixiang Wu, Hongqin Wang, Duo Li, Xu-Feng Huang
Teasaponin Reduces Inflammation And Central Leptin Resistance In Diet-Induced Obese Male Mice, Yinghua Yu, Yizhen Wu, Alexander Szabo, Zhixiang Wu, Hongqin Wang, Duo Li, Xu-Feng Huang
Illawarra Health and Medical Research Institute
Chronic inflammation is involved in the pathogenesis of obesity and type 2 diabetes. Recently, teasaponin, an extract from tea, has been shown to have anti-inflammatory effects. We examined the effect of teasaponin on obesity, inflammation, glucose metabolism and central leptin sensitivity, in obese mice fed a high-fat (HF) diet for 16 weeks. Intraperitoneal injections of teasaponin (10mg/kg, daily) for 21 days significantly decreased the food intake and body weight of HF diet-induced obese mice. Teasaponin treatment also reduced the protein levels of pro-inflammatory cytokines (TNF-α, IL-6 and/or IL-1β) and NF-κB signaling (p-IKK and p-IκBα) in adipose tissue and the liver. …
Selective Depletion Of Alloreactive T Cells Leads To Long-Term Islet Allograft Survival Across A Major Histocompatibility Complex Mismatch In Diabetic Mice, Min Hu, J Wu, G Y. Zhang, Y M. Wang, D Watson, S Yi, W J. Hawthorne, P J. O'Connell, S I. Alexander
Selective Depletion Of Alloreactive T Cells Leads To Long-Term Islet Allograft Survival Across A Major Histocompatibility Complex Mismatch In Diabetic Mice, Min Hu, J Wu, G Y. Zhang, Y M. Wang, D Watson, S Yi, W J. Hawthorne, P J. O'Connell, S I. Alexander
Faculty of Science, Medicine and Health - Papers: part A
Islet cell transplantation as a therapy for type 1 diabetes has been limited by progressive graft loss. Significant immunosuppression including T-cell ablation has been used in an attempt to limit islet rejection. Here, we show that CD3+ lymphocytes depleted of alloreactive T cells selected from a mixed lymphocyte reaction (MLR), where responder BALB/c splenocytes stained with carboxyfluorescein succinimidyl ester (CFSE) were stimulated with irradiated C57BL/6 splenocytes for 5 days, infused into diabetic immunodeficient mice are capable of restoring a broad T-cell repertoire and specifically do not reject islet transplants from the strain (C57BL/6) used in the original depletion. These mice …
Distinct Neurobehavioural Effects Of Cannabidiol In Transmembrane Domain Neuregulin 1 Mutant Mice, Leonora E. Long, Rose Chesworth, Xu-Feng Huang, Alexander Wong, Adena Spiro, Iain S. Mcgregor, Jonathon Arnold, Tim Karl
Distinct Neurobehavioural Effects Of Cannabidiol In Transmembrane Domain Neuregulin 1 Mutant Mice, Leonora E. Long, Rose Chesworth, Xu-Feng Huang, Alexander Wong, Adena Spiro, Iain S. Mcgregor, Jonathon Arnold, Tim Karl
Illawarra Health and Medical Research Institute
The cannabis constituent cannabidiol (CBD) possesses anxiolytic and antipsychotic properties. We have previously shown that transmembrane domain neuregulin 1 mutant (Nrg1 TM HET) mice display altered neurobehavioural responses to the main psychoactive constituent of cannabis, D9-tetrahydrocannabinol. Here we investigated whether Nrg1 TM HET mice respond differently to CBD and whether CBD reverses schizophrenia-related phenotypes expressed by these mice. Adult male Nrg1 TM HET and wild type-like littermates (WT) received vehicle or CBD (1, 50 or 100 mg/kg i.p.) for 21 days. During treatment and 48 h after withdrawal we measured behaviour, whole blood CBD concentrations and autoradiographic receptor binding. Nrg1 …
Metabolic Parameters And Emotionality Are Little Affected In G-Protein Coupled Receptor 12 (Gpr12) Mutant Mice, Elisabeth Frank, Yizhen Wu, Naomi Piyaratna, William James Body, P Snikeris, Timothy South, Anna-Karin Gerdin, Mikael Bjursell, Mohammad Bohlooly-Y, Leonard H. Storlien, Xu-Feng Huang
Metabolic Parameters And Emotionality Are Little Affected In G-Protein Coupled Receptor 12 (Gpr12) Mutant Mice, Elisabeth Frank, Yizhen Wu, Naomi Piyaratna, William James Body, P Snikeris, Timothy South, Anna-Karin Gerdin, Mikael Bjursell, Mohammad Bohlooly-Y, Leonard H. Storlien, Xu-Feng Huang
Faculty of Health and Behavioural Sciences - Papers (Archive)
Background: G-protein coupled receptors (GPR) bear the potential to serve as yet unidentified drug targets for psychiatric and metabolic disorders. GPR12 is of major interest given its putative role in metabolic function and its unique brain distribution, which suggests a role in emotionality and affect. We tested Gpr12 deficient mice in a series of metabolic and behavioural tests and subjected them to a well-established high-fat diet feeding protocol. Methodology/Principal Findings: Comparing the mutant mice with wild type littermates, no significant differences were seen in body weight, fatness or weight gain induced by a high-fat diet. The Gpr12 mutant mice displayed …
Cognitive Phenotyping Of Amyloid Precursor Protein Transgenic J20 Mice, Tim Karl, Surabhi Bhatia, David Cheng, Woojin Scott Kim, Brett Garner
Cognitive Phenotyping Of Amyloid Precursor Protein Transgenic J20 Mice, Tim Karl, Surabhi Bhatia, David Cheng, Woojin Scott Kim, Brett Garner
Illawarra Health and Medical Research Institute
Transgenic mice that express familial Alzheimer's disease mutant forms of the human amyloid precursor protein (hAPP) have proved to be invaluable in determining the impact that the neurotoxic amyloid-β peptide has in vivo. In addition to the propensity to accumulate cerebral amyloid plaques, a crucial characteristic of hAPP mouse models is their cognitive impairments. To date the most widely used test for analyzing cognitive impairment in hAPP mice is the Morris water maze (MWM) which, due to the fact that mice are not "natural" swimmers, may not always be the ideal paradigm to investigate cognitive behaviours. Furthermore, not all cognitive …
Molecular Characterization Of The Onset And Progression Of Colitis In Inoculated Interleukin-10 Gene-Deficient Mice: A Role For Pparcx, Bianca Knoch, Matthew Barnett, Janine Cooney, Warren Mcnabb, Diane Barraclough, William Laing, Shuotun Zhu, Zaneta A. Park, Paul Maclean, Scott O. Knowles, Nicole Roy
Molecular Characterization Of The Onset And Progression Of Colitis In Inoculated Interleukin-10 Gene-Deficient Mice: A Role For Pparcx, Bianca Knoch, Matthew Barnett, Janine Cooney, Warren Mcnabb, Diane Barraclough, William Laing, Shuotun Zhu, Zaneta A. Park, Paul Maclean, Scott O. Knowles, Nicole Roy
Illawarra Health and Medical Research Institute
The interleukin-10 gene-deficient (Il10−/−) mouse is a model of human inflammatory bowel disease and Ppara has been identified as one of the key genes involved in regulation of colitis in the bacterially inoculated Il10−/− model. The aims were to (1) characterize colitis onset and progression using a histopathological, transcriptomic, and proteomic approach and (2) investigate links between PPARα and IL10 using gene network analysis. Bacterial inoculation resulted in severe colitis in Il10−/− mice from 10 to 12 weeks of age. Innate and adaptive immune responses showed differences in gene expression relating to colitis severity. Actin cytoskeleton dynamics, innate immunity, and …
A Behavioural Comparison Of Acute And Chronic Δ9-Tetrahydrocannabinol And Cannabidiol In C57bl/6jarc Mice, Ian Mcgregor, Xu-Feng Huang, Tim Karl, Jonathon Arnold, Rose Chesworth, Leonora E. Long
A Behavioural Comparison Of Acute And Chronic Δ9-Tetrahydrocannabinol And Cannabidiol In C57bl/6jarc Mice, Ian Mcgregor, Xu-Feng Huang, Tim Karl, Jonathon Arnold, Rose Chesworth, Leonora E. Long
Faculty of Health and Behavioural Sciences - Papers (Archive)
Cannabis contains over 70 unique compounds and its abuse is linked to an increased risk of developing schizophrenia. The behavioural profiles of the psychotropic cannabis constituent Δ9-tetrahydrocannabinol (Δ9-THC) and the non-psychotomimetic constituent cannabidiol (CBD) were investigated with a battery of behavioural tests relevant to anxiety and positive, negative and cognitive symptoms of schizophrenia. Male adult C57BL/6JArc mice were given 21 daily intraperitoneal injections of vehicle, Δ9-THC (0.3, 1, 3 or 10 mg/kg) or CBD (1, 5, 10 or 50 mg/kg). Δ9-THC produced the classic cannabinoid CB1 receptor-mediated tetrad of hypolocomotion, analgesia, …
Changes In Colon Gene Expression Associated With Increased Colon Inflammation In Interleukin-10 Gene-Deficient Mice Inoculated With Enterococcus Species, Matthew Pg Barnett, Warren C. Mcnabb, Adrian L. Cookson, Shuotun Zhu, Marcus Davy, Bianca Knoch, Katie Nones, Alison J. Hodgkinson, Nicole Roy
Changes In Colon Gene Expression Associated With Increased Colon Inflammation In Interleukin-10 Gene-Deficient Mice Inoculated With Enterococcus Species, Matthew Pg Barnett, Warren C. Mcnabb, Adrian L. Cookson, Shuotun Zhu, Marcus Davy, Bianca Knoch, Katie Nones, Alison J. Hodgkinson, Nicole Roy
Illawarra Health and Medical Research Institute
Background: Inappropriate responses to normal intestinal bacteria may be involved in the development of Inflammatory Bowel Diseases (IBD, e.g. Crohn's Disease (CD), Ulcerative Colitis (UC)) and variations in the host genome may mediate this process. IL-10 gene-deficient (Il10-/-) mice develop CD-like colitis mainly in the colon, in part due to inappropriate responses to normal intestinal bacteria including Enterococcus strains, and have therefore been used as an animal model of CD. Comprehensive characterization of changes in cecum gene expression levels associated with inflammation in the Il10-/- mouse model has recently been reported. Our aim was to characterize changes in colonic gene …
Dietary Fish Oil Is Anti-Hypertrophic But Does Not Enhance Post-Ischemic Myocardial Function In Female Mice, Catherine E. Huggins, Claire L. Curl, Ruchi Patel, Peter L. Mclennan, Mandy L. Theiss, Thierry Pedrazzini, Salvatore Pepe, Lea M.D. Delbridge
Dietary Fish Oil Is Anti-Hypertrophic But Does Not Enhance Post-Ischemic Myocardial Function In Female Mice, Catherine E. Huggins, Claire L. Curl, Ruchi Patel, Peter L. Mclennan, Mandy L. Theiss, Thierry Pedrazzini, Salvatore Pepe, Lea M.D. Delbridge
Graduate School of Medicine - Papers (Archive)
Clinically and experimentally, a case for omega-3 polyunsaturated fatty acid (PUFA) cardioprotection in females has not been clearly established. The goal of this study was to investigate whether dietary omega-3 PUFA supplementation could provide ischemic protection in female mice with an underlying genetic predisposition to cardiac hypertrophy. Mature female transgenic mice (TG) with cardiac-specific overexpression of angiotensinogen that develop normotensive cardiac hypertrophy and littermate wild-type (WT) mice were fed a fish oil-derived diet (FO) or PUFA-matched control diet (CTR) for 4 wk. Myocardial membrane lipids, ex vivo cardiac performance (intraventricular balloon) after global no-flow ischemia and reperfusion (15/30 min), and …
Beta-2 Glycoprotein I And Its Role In Antiphospholipid Syndrome: Lessons From Knockout Mice, Spyridon Miyakis, Sarah A. Robertson, S A. Krilis
Beta-2 Glycoprotein I And Its Role In Antiphospholipid Syndrome: Lessons From Knockout Mice, Spyridon Miyakis, Sarah A. Robertson, S A. Krilis
Faculty of Science, Medicine and Health - Papers: part A
The antiphospholipid syndrome is characterized by the presence in serum of autoantibodies against h2GPI. Although the role of h2GPI in the pathogenesis of antiphospholipid antibody syndrome (APS) is well recognized, its exact physiological functions still remain undisclosed. Several interactions of h2GPI with components of the coagulation cascade have been proposed, resulting in both procoagulant and anticoagulant effects. Additionally, h2GPI has been implicated in the mechanism of recurrent fetal loss entailed in APS. Recently, using a homologous recombination approach, reproduction of mice homozygous for deletion of the b2GPI gene has been feasible. h2GPI knockout mice offer a valuable tool for revealing …
Immune Responses Of A Liposome/Iscom Vaccine Adjuvant Against Streptococcal Fibronectin Binding Protein 1 (Sfb1) In Mice, Jason D. Mcarthur, K Schulze, James Chin, B J. Currie, K S. Sriprakash, S R. Talay, G S. Chhatwal, C A. Guzman, Mark J. Walker
Immune Responses Of A Liposome/Iscom Vaccine Adjuvant Against Streptococcal Fibronectin Binding Protein 1 (Sfb1) In Mice, Jason D. Mcarthur, K Schulze, James Chin, B J. Currie, K S. Sriprakash, S R. Talay, G S. Chhatwal, C A. Guzman, Mark J. Walker
Faculty of Science, Medicine and Health - Papers: part A
BACKGROUND & OBJECTIVES: The fibronectin binding protein Sfb1 of Streptococcus pyogenes is a well characterised antigen which induces protection against lethal challenge with group A streptococcus (GAS) when adjuvanted with cholera toxin B-subunit (CTB). As an alternative to CTB adjuvanted intranasal immunisations we investigated the immune responses generated in mice using Sfb1 incorporated in to the skin and mucosal adjuvant SAMA4. METHODS: Mice (BALB/c) were vaccinated intradermally with 100 microl of either SAMA4 (adjuvant only group) or SAMA4/Sfb1 and were boosted 7 days later. Mice vaccinated with CTB based vaccines were immunised by intranasal inoculation with a mixture containing 30 …