Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Medicine and Health Sciences
Paradoxical Effects Of The Autophagy Inhibitor 3-Methyladenine On Docetaxel-Induced Toxicity In Pc-3 And Lncap Prostate Cancer Cells, Rebecca Pickard, Briohny Spencer, Amelia Mcfarland, Nijole Bernaitis, Andrew Davey, Russ Chess-Williams, Catherine Mcdermott, Amanda Forbes, David Christie, Shailendra Anoopkumar-Dukie
Paradoxical Effects Of The Autophagy Inhibitor 3-Methyladenine On Docetaxel-Induced Toxicity In Pc-3 And Lncap Prostate Cancer Cells, Rebecca Pickard, Briohny Spencer, Amelia Mcfarland, Nijole Bernaitis, Andrew Davey, Russ Chess-Williams, Catherine Mcdermott, Amanda Forbes, David Christie, Shailendra Anoopkumar-Dukie
Catherine M. McDermott
Survival time in patients with androgen-resistant prostate cancer. However, it provides only a modest increase in survival and is associated with significant toxicity. Therefore, there is an urgent need to identify potential adjunct therapies. Given the key role of autophagy in both tumour survival and chemoresistance, the impact of autophagy modulation on docetaxel toxicity was tested in vitro. PC-3 and LNCaP cells were pre-treated with the autophagy inhibitor 3- methyladenine (5 mM) and then exposed to various concentrations (0–100 μM) of docetaxel. Cytoxic effects of docetaxel were measured using resazurin reduction to resorufin, whilst autophagy and apoptosis was measured using …
Enhanced Urothelial Atp Release And Contraction Following Intravesical Treatment With The Cytotoxic Drug, Doxorubicin, Stanley Kang, Catherine Mcdermott, Stef Farr, R. Chess-Williams
Enhanced Urothelial Atp Release And Contraction Following Intravesical Treatment With The Cytotoxic Drug, Doxorubicin, Stanley Kang, Catherine Mcdermott, Stef Farr, R. Chess-Williams
Catherine M. McDermott
Intravesical administration of the cytotoxic drug doxorubicin is a common treatment for superficial carcinoma of the bladder, but it is associated with significant urological adverse effects. The aim of this study was to identify doxorubicin-induced changes in the local mechanisms involved in regulating bladder function. As a model of intravesical doxorubicin administration in patients, doxorubicin (1 mg/mL) was applied to the luminal surface of porcine bladders for 60 min. Following treatment, the release of urothelial/lamina propria mediators (acetylcholine (Ach), ATP and prostaglandin E2 (PGE2) and contractile responses of isolated tissue strips was investigated. Doxorubicin pretreatment did not affect contractile responses …
Induction Of Inflammatory Cytokines And Alteration Of Urothelial Atp, Acetylcholine And Prostaglandin E2 Release By Doxorubicin, Sung-Hung Kang, Russ Chess-Williams, Shailendra Anoopkumar-Dukie, Catherine Mcdermott
Induction Of Inflammatory Cytokines And Alteration Of Urothelial Atp, Acetylcholine And Prostaglandin E2 Release By Doxorubicin, Sung-Hung Kang, Russ Chess-Williams, Shailendra Anoopkumar-Dukie, Catherine Mcdermott
Catherine M. McDermott
Intravesical treatment with cytotoxic drugs such as doxorubicin is associated with local adverse effects in bladder cancer patients. Here we investigate the effects of doxorubicin on urothelial release of ATP, acetylcholine and prostaglandin E2, and production of inflammatory cytokines. Urothelial cells were treated with doxorubicin for 1 h at 37 °C. Immediately or 24 h following treatment the level of ATP, acetylcholine and prostaglandin E2 released under basal and stimulated conditions was measured and compared to release from vehicle treated control cultures. The presence of inflammatory cytokines, in culture medium was also assessed 24 h after doxorubicin pre-treatment. Immediately following …