Medicine and Health Sciences Commons^™

Detection Of Productively Rearranged Tcr-Α V–J Sequences In Tcga Exome Files: Implications For Tumor Immunoscoring And Recovery Of Antitumor T-Cells, Thomas R. Gill, Mohammad D. Samy, Shanitra N. Butler, James A. Mauro, Wade J. Sexton, George Blanck

Molecular Medicine Faculty Publications

Tumor immunoscoring is rapidly becoming a universal parameter of prognosis, and T-cells isolated from tumor masses are used for ex vivo amplification and readministration to patients to facilitate an antitumor immune response. We recently exploited the cancer genome atlas (TCGA) RNASeq data to assess T-cell receptor (TcR) expression and, in particular, discovered strong correlations between major histocompatibility class II (MHCII) and TcR-α constant region expression levels. In this article, we describe the results of searching TCGA exome files for TcR-α V-regions, followed by searching the V-region datasets for TcR-α-J regions. Both primary and metastatic breast cancer sample files contained recombined …