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An Overview Of Alcohol Testing And Interpretation In The 21st Century, Anna Kelly, Ashraf Mozayani
An Overview Of Alcohol Testing And Interpretation In The 21st Century, Anna Kelly, Ashraf Mozayani
Ashraf Mozayani, Ph.D., PharmD
Ethanol analysis is the most commonly carried out drug testing in a forensic toxicology laboratory. Determination of blood alcohol concentration (BAC) is needed in a multitude of situations, including in postmortem analysis, driving under the influence (DUI) and drug-facilitated sexual assault (DFSA) cases, workplace drug monitoring, and probation investigations. These analyses are carried out by direct measurement of ethanol concentrations as well as of metabolic by-products, such as ethyl glucuronide (EtG) and ethyl sulfate (EtS). This review article will discuss pharmacokinetics, including absorption, distribution, and elimination of ethanol, methods for the detection of ethanol, the effect of ethanol on human …
Validation Of Lc-Tof/Ms Screening For Drugs, Metabolites, And Collateral Compounds In Forensic Toxicology Specimens, Fessessework Guale, Shahriar Shahreza, Jeffrey Walterscheid, Hsin-Hung Chen, Crystal Arndt, Anna Kelly, Ashraf Mozayani
Validation Of Lc-Tof/Ms Screening For Drugs, Metabolites, And Collateral Compounds In Forensic Toxicology Specimens, Fessessework Guale, Shahriar Shahreza, Jeffrey Walterscheid, Hsin-Hung Chen, Crystal Arndt, Anna Kelly, Ashraf Mozayani
Ashraf Mozayani, Ph.D., PharmD
Liquid chromatography time-of-flight mass spectrometry (LC-TOF-MS) analysis provides an expansive technique for identifying many known and unknown analytes. This study developed a screening method that utilizes automated solid-phase extraction to purify a wide array of analytes involving stimulants, benzodiazepines, opiates, muscle relaxants, hypnotics, antihistamines, antidepressants and newer synthetic "Spice/K2" cannabinoids and cathinone "bath salt" designer drugs. The extract was applied to LC-TOF-MS analysis, implementing a 13 min chromatography gradient with mobile phases of ammonium formate and methanol using positive mode electrospray. Several common drugs and metabolites can share the same mass and chemical formula among unrelated compounds, but they are …