Medicine and Health Sciences Commons^™

Investigating Propargyl-Linked Antifolates In Inhibiting Bacterial And Fungal Dihydrofolate Reductase, Joshua Andrade

Honors Scholar Theses

Antimicrobial agents have been invaluable in reducing illness and death associated with bacterial infection. However, over time, bacteria have evolved resistance to all major drug classes as a result of selective pressure. The advancement of new drug compounds is therefore vital. The Anderson-Wright Lab has focused on developing potent and selective inhibitors of dihydrofolate reductase (DHFR), an enzyme key in cell proliferation and survival, in several pathogenic species. The lab has found that a set of compounds, known as propargyl-linked antifolates, are DHFR inhibitors that are both biologically effective and have strong pharmacokinetic properties.

The efficacy of novel propargyl-linked antifolates …

Theta And Learning: Dorsal And Ventral Hippocampal Theta Oscillation Respond Differently To Learning, Gregory N. Newman

Honors Scholar Theses

Rhythmic oscillations within the hippocampus are thought to synchronize various subregions during learning, maximizing efficiency of the neural circuits. In rats, the most prominent oscillation is hippocampal theta. Theta is known to be modulated by an animal’s velocity, but it has also been shown to change in response to cognitive demands. To determine if theta is important for learning and memory, EEG recordings were examined for changes in theta related to the decision point of a spatial or non-spatial T-maze task. Additionally, a straight runway served as a control to verify the consistent relationship between velocity and theta during a …

Chemical Profiling And Biological Activity Of Two Tunicate-Associated Marine Bacteria, Lyubina Yankova

Honors Scholar Theses

Marine natural products have recently been an increasingly abundant source of novel antibiotics. Given that there is an increasing resistance to current drug therapies, finding new sources such as marine natural products is essential. Tunicate-associated marine bacteria can be a significant source of antibacterial compounds. Two tunicates of the species Eudistoma were collected from Portobelo National Park on the Salmedina Reef of Panama in the Caribbean Sea. Bacteria associated with the tunicate were isolated, cultured, extracted, and fractionated. Fractions were tested against an array of clinically relevant bacterial pathogens in the BioMAP assay. Two fractions MB0086E and MB0088E demonstrated activity …

Relationship Between Sensory Sensitivities And Cognitive And Adaptive Abilities In Children With Autism Spectrum Disorders, Laura Pendergast

Honors Scholar Theses

Sensory sensitivities are widely reported among individuals with ASD. These sensory sensitivities can be classified as over-responsivity, under-responsivity, or seeking of sensory stimulation. Following recent changes in the diagnostic criteria, sensory sensitivities are considered a key feature of the behavioral phenotype of ASDs. Despite their significance, sensory sensitivities have been largely underestimated. Therefore, more research in this area may reveal important information about the influence of sensitivities on functioning, as well as the underlying causes of the symptoms. This study investigated a possible relationship between sensory sensitivities and cognitive and adaptive abilities in children with ASD. The sample included 29 …

The Reversal Effects Of Curcumin, An Herbal Remedy, On The Impairments Induced By Vmat-2 Inhibitor Tetrabenazine, Emily Qian, Samantha E. Yohn

Honors Scholar Theses

Substantial evidence has shown that dopamine (DA), particularly in the nucleus accumbens (NAc), is involved in behavioral activation and effort-related processes, such as overcoming work related response costs. Interference with accumbens DA transmission through administration of the vesicular monoamine transportor-2 (VMAT-2) inhibitor tetrabenazine (TBZ) produces an alteration of response allocation in the concurrent FR5/chow choice procedure, biasing animals toward the lower effort alternative. It has been suggested that these drug-induced shifts in effort-related choice behavior seen in rodents are analogous to symptoms such as psychomotor retardation, anergia, and fatigue, which can be observed in people with depression and other related …

Modeling The Adaptive Immune Response To Mutation-Generated Antigens, Rory J. Geyer

Honors Scholar Theses

Somatic mutations may drive tumorigenesis or lead to new, immunogenic epitopes (neoantigens). The immune system is thought to represses neoplastic growths through the recognition of neoantigens presented only by tumor cells. To study mutations as well as the immune response to mutation-generated antigens, we have created a conditional knockin mouse line with a gene encoding, 5’ to 3’, yellow fluorescent protein (YFP), ovalbumin (which is processed to the immunologically recognizable peptide, SIINFEKL), and cyan fluorescent protein (CFP), or, YFP-ovalbumin-CFP. A frame shift mutation has been created at the 5’ end of the ovalbumin gene, hence YFP should always be expressed, …

Assessing The Relationship Between The Blood-Air Partition Coefficient And Fractional Uptake Of Inspired Halothane, Acetone, And Ethanol Vapors In The Airways Of The C57bl/6j Mouse, Joshua Baruch Baldino

Honors Scholar Theses

The following series of studies investigates the elimination and uptake trends of halothane, acetone, and ethanol vapors in the airways of C57BL/6J mice. These vapors were chosen because they span a wide range of solubilities, as indicated by their blood-air partition coefficients, and are not associated with any significant airway metabolism or reactivity with tissue substrates in vivo. Mice were exposed to a homogeneous vapor mixture containing a 1:1:1 ratio of halothane, acetone, and ethanol at relative concentrations of approximately 10 ppm. Exposure studies were performed with mice in two states, conscious and deceased, in order to provide control …

The Effect Of Repeated Mild Traumatic Brain Injury On Ventricular Volume And Microglial Activation, Lillian Rose Talbot

Honors Scholar Theses

As the leading cause of death and disability in individuals under the age of 45-years-old, Traumatic Brain Injury (TBI) is a public health crisis that demands the attention of the scientific and medical community [28]. The majority of all TBIs that occur in the United States each year are a non-deadly yet detrimental form of closed brain injury known as mild TBI (mTBI) or concussion [6]. Athletes, young people and military personnel all face a high risk of acquiring mTBI as a result of their environments. In our study we have chosen to model repeated mTBI (rmTBI) in the mouse …

Factors Associated With Abnormal Weights In Infants In The First Year Of Life: Results From The 2010 National Ambulatory Medical Care Survey, Nikaela R. Larossa

Honors Scholar Theses

Purpose: To examine the predictors associated with abnormal infant weights for lengths in the first year of life as seen in ambulatory care settings.

Data Sources: A secondary data analysis was conducted using the 2010 National Ambulatory Medical Care Survey (NAMCS), in which practitioners documented single patient visits. World Health Organization (WHO) growth charts and recommendations were used to evaluate infant weight for length. Of the total visits, 603 infants were less than one year old, and 595 infants were within the length range of the WHO growth charts. Of the 595 cases, 13.6% were underweight, 5.5% were overweight, 41.2% …

Effects On Pinniped Immune Response Upon In Vitro Exposure To The Perfluorinated Compounds, Pfos And Pfoa, Elizabeth J. Meiman

Honors Scholar Theses

This study investigated the effects of environmental toxicants on the immune system of two pinniped species, grey seals (Halichoerus grypus) and hooded seals (Cystophora cristata). The toxicants included two perfluorinated compounds (PFC), perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS), compounds commonly found in a wide variety of household consumer products, including Scotchguard and Teflon. Although corporations such as 3M curtailed the use of these chemicals in the past decade, concentrations of these chemicals are increasing in the arctic aquatic ecosystem and have been measured in the tissues and blood of arctic pinnipeds. However, the effects of …

An Animal Model Of The Motivational Symptoms Of Depression: Testing The Antidepressant Desipramine On An Effort-Related Choice Task, Samantha L. Collins

Honors Scholar Theses

Patients with depression, schizophrenia, and other related disorders often show effort-related motivational symptoms such as anergia, psychomotor slowing, lassitude, and fatigue. Several studies have indicated that dopamine (DA) within the nucleus accumbens (NAc) is involved in the regulation of effort-related behavior. Interference with NAc DA alters response allocation in effort related choice procedures, biasing animals towards the alterative that can be obtained with minimal effort. Previous studies have shown that administration of the vesicular monoamine transporter-2 (VMAT-2) inhibitor tetrabenazine (TBZ) shifts behavior in rats responding on the FR5/chow choice procedure causing a decrease in lever pressing and a compensatory increase …

The Role Of Amp-Activated Protein Kinase (Ampk) In Regulating The Early Stages Of Acute Pancreatitis, Mohamed Shitia

Honors Scholar Theses

Acute pancreatitis is an inflammatory disease that is initiated by the activation and retention of digestive zymogens inside pancreatic acinar cells (acini). It is proposed that adenosine monophosphate-activated protein kinase (AMPK) regulates early responses of acute pancreatitis in acini. A recent study shows that induction of experimental pancreatitis in isolated rat acini with supraphysiologic cerulein (orthologue of cholecystokinin) increases intracellular zymogen activation, but decreases AMPK levels. Furthermore, in vitro pharmacologic stimulation of AMPK reduces zymogen activation, having a protective effect. In this study, the effectiveness of two AMPK activators was examined in two separate in vivo pancreatitis models. In the …

Validity And Reliability Testing Of The National Health And Nutrition Examination Survey (Nhanes) Taste And Smell Protocol, Mallory Honda

Honors Scholar Theses

The NHANES 2011–2014 protocol includes a taste and smell questionnaire (CSQ) in the home interview followed by brief olfactory and taste assessment in mobile exam centers. The CSQ asks self-reported taste and smell ability, and selected symptoms, comorbidities, and treatment for chemosensory disorders. In the taste assessment, participants rate intensities of 1 M NaCl and 1mM quinine hydrochloride applied to the tongue tip and these plus 0.32M NaCl sampled with the whole mouth. Smell function is assessed with two 4-item, scratch-and-sniff tests (Pocket Tests^TM (PT), Sensonics, Inc.) to classify normosmia and olfactory dysfunction from microsmia to anosmia. We examined …

Cultural Competency In The Medical Workplace: A Look At Outpatient Clinic Nurses At A Children's Hospital In New England, Evelyn S. Callahan

Honors Scholar Theses

This paper analyzes the current state of progress toward cultural competency in the medical workplace, specifically in the hospital setting. It compares the current writing on the topic to research done at a large New England children’s hospital. The nurses are all individuals who work in an out patient setting so they often see the same patients regularly for longer periods of time. This differs from the in-patient or floor nurses who only spend limited time with a constantly changing population of patients. The research involved one-on-one interviews and a focus group with nurses at the hospital. The focus group …

An Examination Of Correctional Nursing Competencies For Improved Patient Care, Bing Zheng

Honors Scholar Theses

The concept of this honors project was to explore correctional nursing competency, working closely with my advisor and the research team. Key findings from the initial competency assessment evaluation phase were compiled, organized, and analyzed. A podium presentation “Advancing Correctional Nurse Competencies for Quality Care: Evaluation of Simulation Learning and Satisfaction” was given at the 2013 American Correctional Health Services Association (ACHSA) Multidisciplinary Educational Conference on Correctional Healthcare: Ring a Bell. Additionally, a poster presentation, “Correctional Nurse Competencies: Evaluation of Simulation Learning and Satisfaction,” was given at the 2013 Frontiers conference. A poster presentation was given at the University of …