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Full-Text Articles in Medicine and Health Sciences
Comparative Evaluation Of Indirect Enzyme Linked Immunosorbent Assay, Rose Bengal Plate Test, Microagglutination Test, And Polymerase Chain Reaction For Diagnosis Of Brucellosis In Buffaloes, Malik Raies Ul Islam, Mohinder Pratap Gupta, Pritam Kaur Sidhu, Gursimran Filia, Hari Mohan Saxena, Tawheed Ahmed Shafi
Comparative Evaluation Of Indirect Enzyme Linked Immunosorbent Assay, Rose Bengal Plate Test, Microagglutination Test, And Polymerase Chain Reaction For Diagnosis Of Brucellosis In Buffaloes, Malik Raies Ul Islam, Mohinder Pratap Gupta, Pritam Kaur Sidhu, Gursimran Filia, Hari Mohan Saxena, Tawheed Ahmed Shafi
Turkish Journal of Veterinary & Animal Sciences
In the present study, 178 blood samples from buffaloes were tested against indirect enzyme linked immunosorbent assay (I-ELISA), rose bengal plate test (RBPT), microagglutination test (MAT), modified microagglutination test (mMAT), and polymerase chain reaction (PCR) to select the most suitable test for efficient and effective diagnosis of bovine brucellosis. Win Episcope 2 software was used to determine the agreement between tests (kappa values at 95% confidence interval). I-ELISA was pair compared with all the other tests. Out of 178 samples, 102 were found positive by I-ELISA, 81 by RBPT, 85 by MAT, 79 by mMAT, and 68 by PCR. Substantial …
Seroprevalence Of Brucellosis In Livestock Within Three Endemic Regions Of The Country Of Georgia, Eliso Mamisashvili, Ian T. Kracalik, Tinatin Onashvili, Lela Kerdzevadze, Ketevan Goginashvili, Tamar Tigilauri, Marina Donduashvili, Marina Nikolaishvili, Irma Beradze, Marina Zakareishvili, Maka Kokhreidze, Makvala Gelashvili, Nino Vepkhvadze, S. Elizabeth Rácz, Philip H. Elzer, Mikeljon P. Nikolich, Jason K. Blackburn
Seroprevalence Of Brucellosis In Livestock Within Three Endemic Regions Of The Country Of Georgia, Eliso Mamisashvili, Ian T. Kracalik, Tinatin Onashvili, Lela Kerdzevadze, Ketevan Goginashvili, Tamar Tigilauri, Marina Donduashvili, Marina Nikolaishvili, Irma Beradze, Marina Zakareishvili, Maka Kokhreidze, Makvala Gelashvili, Nino Vepkhvadze, S. Elizabeth Rácz, Philip H. Elzer, Mikeljon P. Nikolich, Jason K. Blackburn
Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications
Brucellosis is the one of most common livestock zoonoses in Georgia, resulting in significant economic losses. Livestock were sampled in three regions of Georgia (Kakheti, Kvemo Kartli, Imereti). Districts that historically reported high numbers of brucellosis related morbidity were selected for serological, bacteriological and molecular surveys. Surveying efforts yielded samples from 10,819 large and small ruminants. In total, 735 serological tests were positive on Rose Bengal and 33 bacterial isolates were recovered and identified as Brucella melitensis or Brucella abortus by microbiology and AMOS-PCR. A Bayesian framework was implemented to estimate the true prevalence of the disease given an imperfect …