Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 4 of 4
Full-Text Articles in Medicine and Health Sciences
Scd1 Plays A Tumor-Suppressive Role In Survival Of Leukemia Stem Cells And The Development Of Chronic Myeloid Leukemia, H Zhang, H Li, N Ho, Dongguang Li, Shaoguang Li
Scd1 Plays A Tumor-Suppressive Role In Survival Of Leukemia Stem Cells And The Development Of Chronic Myeloid Leukemia, H Zhang, H Li, N Ho, Dongguang Li, Shaoguang Li
Research outputs 2012
Chronic myeloid leukemia (CML) is derived from a stem cell, and it is widely accepted that the existence of leukemia stem cells (LSCs) is one of the major reasons for the relapse of CML treated with kinase inhibitors. Key to eradicating LSCs is to identify genes that play a critical role in survival regulation of these stem cells. Using BCR-ABL-induced CML mouse model, here we show that expression of the stearoyl-CoA desaturase 1 (Scd1) gene is downregulated in LSCs and that Scd1 plays a tumor-suppressive role in LSCs with no effect on the function of normal hematopoietic stem cells. Deletion …
Evaluation Of A Multi-Marker Immunomagnetic Enrichment Assay For The Quantification Of Circulating Melanoma Cells, James Freeman, Elin Gray, Michael Milward, Robert Pearce, Melanie Ziman
Evaluation Of A Multi-Marker Immunomagnetic Enrichment Assay For The Quantification Of Circulating Melanoma Cells, James Freeman, Elin Gray, Michael Milward, Robert Pearce, Melanie Ziman
Research outputs 2012
Background: Circulating melanoma cells (CMCs) are thought to be valuable in improving measures of prognosis in melanoma patients and may be a useful marker of residual disease to identify non-metastatic patients requiring adjuvant therapy. We investigated whether immunomagnetic enrichment targeting multiple markers allows more efficient enrichment of CMCs from patient peripheral blood than targeting a single marker. Furthermore, we aimed to determine whether the number of CMCs in patient blood was associated with disease stage.Methods: We captured CMCs by targeting the melanoma associated markers MCSP and MCAM as well as the melanoma stem cell markers ABCB5 and CD271, both individually …
Altered Expression Of Alzheimer's Disease-Related Proteins In Male Hypogonadal Mice, Eleanor S. Drummond, Ralph Martins, D J Handelsman, A R Harvey
Altered Expression Of Alzheimer's Disease-Related Proteins In Male Hypogonadal Mice, Eleanor S. Drummond, Ralph Martins, D J Handelsman, A R Harvey
Research outputs 2012
Age-related depletion of estrogens and androgens is associated with an increase in Alzheimer's disease (AD) brain pathology and diminished cognitive function. Here we investigated AD-associated molecular and cellular changes in brains of aged hypogonadal (hpg) male and female mice. hpg Mice have a spontaneous, inactivating genetic mutation in the GnRH gene resulting in lifelong deficiency of gonadotropins and gonadal sex hormones. Western blot analysis revealed low levels of amyloid precursor protein and high levels of presenilin 1, amyloid precursor protein C-terminal fragment, and β-amyloid 42 in brains of aged male, but not female, hpg mice. Changes were confined to the …
The Hippo Pathway Member Yap Plays A Key Role In Influencing Fate Decisions In Muscle Satellite Cells, Robert Judson, Annie Tremblay, Paul Knopp, Robert White, Roby Urcia, Cosimo De Bari, Peter Zammit, Fernando Camargo, Henning Wackerhage
The Hippo Pathway Member Yap Plays A Key Role In Influencing Fate Decisions In Muscle Satellite Cells, Robert Judson, Annie Tremblay, Paul Knopp, Robert White, Roby Urcia, Cosimo De Bari, Peter Zammit, Fernando Camargo, Henning Wackerhage
Research outputs 2012
Satellite cells are the resident stem cells of skeletal muscle. Mitotically quiescent in mature muscle, they can be activated to proliferate and generate myoblasts to supply further myonuclei to hypertrophying or regenerating muscle fibres, or self-renew to maintain the resident stem cell pool. Here, we identify the transcriptional co-factor Yap as a novel regulator of satellite cell fate decisions. Yap expression increases during satellite cell activation and Yap remains highly expressed until after the differentiation versus self-renewal decision is made. Constitutive expression of Yap maintains Pax7+ and MyoD+ satellite cells and satellite cell-derived myoblasts, promotes proliferation but prevents differentiation. In …