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Full-Text Articles in Medicine and Health Sciences
Laboratory Diagnosis Of Mycoplasma Gallisepticum (Mg) Infection In Experimental Layer Chicken Receiving Mg Vaccines And Mg Organisms, Somsak Pakpinyo, Pinyo Pitayachamrat, Seubchat Saccavadit, Than Santaswang, Achara Tawatsin, Jiroj Sasipreeyajan
Laboratory Diagnosis Of Mycoplasma Gallisepticum (Mg) Infection In Experimental Layer Chicken Receiving Mg Vaccines And Mg Organisms, Somsak Pakpinyo, Pinyo Pitayachamrat, Seubchat Saccavadit, Than Santaswang, Achara Tawatsin, Jiroj Sasipreeyajan
The Thai Journal of Veterinary Medicine
This experiment was designed to study the results of laboratory diagnosis of Mycoplasma gallisepticum (MG) infection in layer chicken receiving MG vaccines and MG organisms. One hundred and twenty 1-day-old-malelayer chickens were raised in separate isolation rooms and equally allocated into 4 separated groups. When 4 weeks old, groups 1, 2, 3 and 4 served as a negative control, a MG commercially inactivated vaccine group, a MG commercially live vaccine group and a MG infected group, respectively. In each group, ten birds were swabbed and bled when 4, 5, 6, 7, 8, 9 and 11 weeks old. Swab samples were …
Comparison Of The Diagnostic Value Of The Standard Tube Agglutination Test And The Elisa Igg And Igm In Patients With Brucellosis, Mustafa Ertek, Hali̇l Yazgi, Zülal Özkurt, Ahmet Ayyildiz, Mehmet Parlak
Comparison Of The Diagnostic Value Of The Standard Tube Agglutination Test And The Elisa Igg And Igm In Patients With Brucellosis, Mustafa Ertek, Hali̇l Yazgi, Zülal Özkurt, Ahmet Ayyildiz, Mehmet Parlak
Turkish Journal of Medical Sciences
Background and Aims: Brucellosis is endemic in Turkey. Since it affects many organs and the symptoms are non-specific, the diagnosis by clinical findings is difficult and may be easily missed. Many serological tests have been used for the diagnosis of human brucellosis. This study compared the diagnostic value of the Brucella standard tube agglutination test (SAT) with that of ELISA (Brucella specific IgG and IgM) tests in patients with Brucella bacteremia. Patients and Methods: Thirty-two patients with brucellosis who had positive blood and/or bone-marrow cultures for Brucella species, and 20 healthy individuals as controls were included in the study. Results: …
Application Of Western Blotting And Enzyme Linked Immunosorbent Assay (Elisa) For The Diagnosis Of Dicrocoelium Dendriticum In Sheep Using Excretory Secretory (E/S) Antigens, Sami̇ Şi̇mşek, Ergün Köroğlu, Armağan Erdem Ütük
Application Of Western Blotting And Enzyme Linked Immunosorbent Assay (Elisa) For The Diagnosis Of Dicrocoelium Dendriticum In Sheep Using Excretory Secretory (E/S) Antigens, Sami̇ Şi̇mşek, Ergün Köroğlu, Armağan Erdem Ütük
Turkish Journal of Veterinary & Animal Sciences
Protein bands of excretory/secretory (E/S) antigens of Dicrocoelium dendriticum were determined using SDS-PAGE and Western blotting. The E/S antigens were prepared from live adult D. dendriticum, which were obtained from slaughtered sheep at a local abattoir. In the SDS-PAGE analysis of D. dendriticum E/S antigen, 9 protein bands with molecular weights varying between 6 and 66 kDa with Coomassie staining and 14 bands between 6 and 205 kDa with silver staining were detected. Blood samples were obtained from sheep at the local abattoir. Following the examination of the organs for D. dendriticum and other helminths, serum samples were divided into …
Serological And Virological Investigations Of Bovine Viral Diarrhoea Virus (Bvdv) Infection In Dairy Cattle Herds In Aydın Province, Mehmet Tolga Tan, M. Taner Karaoğlu, Nural Erol, Yakup Yildirim
Serological And Virological Investigations Of Bovine Viral Diarrhoea Virus (Bvdv) Infection In Dairy Cattle Herds In Aydın Province, Mehmet Tolga Tan, M. Taner Karaoğlu, Nural Erol, Yakup Yildirim
Turkish Journal of Veterinary & Animal Sciences
A total of 288 dairy cattle from 4 different closed barns were sampled in order to investigate the presence of bovine viral diarrhoea virus (BVDV) infection. Based on the data gathered from neutralisation peroxidase-linked antibody (NPLA) tests, which were conducted to detect BVDV antibodies, all of the barns monitored previously had the infection; the rates of seropositive animals were between 44% and 100%. Overall, the results obtained from Aydın province showed that 248 of the 288 dairy cows (86%) monitored were seropositive. BVDV antigen ELISA kits (Bio-X Diagnostics- Belgium) were used to detect BVDV antigen (NS3 protein) in blood samples. …
A Comparative Study Of Detection Of Bordetella Avium Antibodies In Turkeys By Elisa, Spat, And Agid Test, Süheyla Türkyilmaz, Kenan Türkyilmaz, Osman Kaya
A Comparative Study Of Detection Of Bordetella Avium Antibodies In Turkeys By Elisa, Spat, And Agid Test, Süheyla Türkyilmaz, Kenan Türkyilmaz, Osman Kaya
Turkish Journal of Veterinary & Animal Sciences
The aims of this study were to develop a serum plate agglutination test (SPAT) antigen and agar gel immunodiffusion (AGID) test antigen for the serological detection of turkeys that have been exposed to Bordetella avium; to compare the sensitivity of commercial enzyme-linked immunosorbent assay (ELISA) with SPAT, and AGID test, and to survey B. avium antibodies in turkey flocks in Aydın, Turkey. For these purposes, serum samples collected from 300 turkeys were examined by ELISA, SPAT, and AGID test. The seroprevalence was 44%, 28%, and 15% for ELISA, SPAT, and AGID test, respectively. This study revealed that ELISA was the …