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Full-Text Articles in Medicine and Health Sciences

Genetically Modified Mosquitoes: A New Strategy To Control Mosquito Borne Diseases, Padet Siriyasatien, Usavadee Thavara Dec 2006

Genetically Modified Mosquitoes: A New Strategy To Control Mosquito Borne Diseases, Padet Siriyasatien, Usavadee Thavara

The Thai Journal of Veterinary Medicine

Mosquito borne diseases remain a major human and animal health concern in many areas of the world. Chemical control strategy faces a number of problems including the development of insecticide resistance in mosquitoes, the impact of insecticides on human and animal health and the environment and the constant inability to control mosquitoes effectively. Advances in the knowledge about mosquito-pathogen relationships and the molecular biology of mosquitoes make it now possible to produce mosquito strains that are unable to transmit various parasites or viruses. Strategies of using the genetically modified mosquitoes to control mosquito borne diseases are discussed here.


Culex Tritaeniorhynchus Is Unlikely To Be A Vector For The Porcine Reproductive And Respiratory Syndrome Virus (Prrsv), Kidsadagon Pringproa, Sudchit Chungpivat, Rapee Panyathong, Roongroje Thanawongnuwech Dec 2006

Culex Tritaeniorhynchus Is Unlikely To Be A Vector For The Porcine Reproductive And Respiratory Syndrome Virus (Prrsv), Kidsadagon Pringproa, Sudchit Chungpivat, Rapee Panyathong, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

At least, 4 mosquito species, Culex tritaeniorhynchus, Culex gelidus, Anopheles vagus and Mansonia uniformis were identified on a pig farm in Nakorn Pathom, Thailand between March 2004 to February 2005. C. tritaeniorhynchus was found to be the predominant species (88.387.63%). To determine whether C. tritaeniorhynchus could serve as a mechanical vector for PRRSV transmission, 2 experiments were conducted on: the duration of PRRSV within the mosquitoes and PRRSV transmission from the PRRSV-infected pigs to naive pigs by the infected mosquitoes. PRRSV nucleic acid could be detected in the whole pooled mosquito samples for up to 48 hours post feeding in …


Thoracoscopic-Guided Lung Biopsy In Dogs, Churee Pramatwinai, Atichat Brahmasa, Suwicha Chuthatep, Pasakorn Birksawan, Kiatpicet Komin Dec 2006

Thoracoscopic-Guided Lung Biopsy In Dogs, Churee Pramatwinai, Atichat Brahmasa, Suwicha Chuthatep, Pasakorn Birksawan, Kiatpicet Komin

The Thai Journal of Veterinary Medicine

The study developed a thoracoscopic-guided lung biopsy technique that was able to be performed on the right or left lung lobes of dogs. Ten experimental dogs weighing 6-20 kilograms were anesthetized, ventilated and positioned in a lateral recumbency. Either the left or right hemithorax was approached. Two instrumental ports were utilized, the first port for a telescope was located at the fifth or sixth intercostal space at a half way distance between the vertebral column and sternum. The second port for the grasping forceps, 12-mm in diameter, was located at either the fourth or sixth or seventh intercostal space at …


What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthateph Dec 2006

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthateph

The Thai Journal of Veterinary Medicine

No abstract provided.


Rapid Isolation Of Dna From The Mucus Of Asian Arowana (Scleropages Formosus, Osteoglossidae), Nantarika Chansue Dec 2006

Rapid Isolation Of Dna From The Mucus Of Asian Arowana (Scleropages Formosus, Osteoglossidae), Nantarika Chansue

The Thai Journal of Veterinary Medicine

Owing to arowana being listed in CITES, DNA diagnostics should not rely on conventional methods of obtaining tissue samples. A non-invasive method of obtaining material containing DNA has been developed. Mucus samples for DNA isolation can be obtained by cotton swabbing the arowana body surface with subsequent purification of DNA fragments by using phenol/chloroform. DNA sample quality is assessed by UV absorbancy at wave lengths of 260 nm and 280 nm, as well as by calculating the absorbancy ratio involving 260 nm/280 nm results in order to determine DNA sample quality and quantity. Electrophoresis is employed to assess DNA quantity …


Morphological Changes In The Oviduct Of Culling Replacement Gilts, Paisan Tienthai, Kriengyot Sajjarengpong, Padet Talummuk Dec 2006

Morphological Changes In The Oviduct Of Culling Replacement Gilts, Paisan Tienthai, Kriengyot Sajjarengpong, Padet Talummuk

The Thai Journal of Veterinary Medicine

The aim of this study was to investigate the morphological changes of the oviduct from 30 culling replacement gilts and 6 control sows. Blood samples and female genital organs were taken immediately after slaughter. Post-mortem examinations were made on the genital organs and oviductal samples, i.e. utero-tubal junction (UTJ), isthmus and ampulla, were collected and fixed for histological analysis. Observations indicated that the reasons for culling the gilts were 'no estrus' (68%) and 'repeat breeding' (32%). The greatest pathological changes (67%) were found in repeat breeding gilts, whereas 52% of no estrous gilts were at prepubertal phase. The female hormonal …


Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Anusak Kijtawornrat Dec 2006

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Anusak Kijtawornrat

The Thai Journal of Veterinary Medicine

No abstract provided.


Ultrasound Diagnosis, Phiwipha Kamonrat Dec 2006

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


Editor’S Note, Ornsiri Cheunsuang Dec 2006

Editor’S Note, Ornsiri Cheunsuang

The Thai Journal of Veterinary Medicine

No abstract provided.


What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthateph Sep 2006

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthateph

The Thai Journal of Veterinary Medicine

No abstract provided.


The Study Of The Radiographic Positionings Of The Canine Elbow For Identifying The Medial Coronoid Process, Suwicha Chuthatep, Kongkiat Srisuwatanasagul, Chalika Wangdee, Kumpanart Sunthornwipart, Surassanan Angkana Sep 2006

The Study Of The Radiographic Positionings Of The Canine Elbow For Identifying The Medial Coronoid Process, Suwicha Chuthatep, Kongkiat Srisuwatanasagul, Chalika Wangdee, Kumpanart Sunthornwipart, Surassanan Angkana

The Thai Journal of Veterinary Medicine

Twenty cadaver canine forelimbs, 9 right and 11 left, were used to evaluate the most effective radiographic position that can enhance the cranial aspect of the medial coronoid process (MCP) of the ulna. A lead pellet (1 mm) was buried to represent the area representing the cartilegenous fragment in the canine patient in the fragmented medial coronoid process (FMCP). The distance between the pellet and the radial surface and the superimposition of the humeral condyle silhouette on the pellet were used as parameters for analysis. The results showed that the disto45 medialproximolateral oblique (Di45M-PrLO) position with a 40 flexed elbow …


Surveillance Of Classical Swine Fever Virus Antibody Titers In Sows And Their Piglets Under Different Vaccination Programs, Achariya Sailasuta, Rachod Tantilertcharoen, Pornchalit Ausawacheep, Sanipa Suradhat, Praveena Kitikoon, Supot Wattanapunsak, Sawang Kesdangsakonwut, Sumittra Wattanodorn, Supasawat Buranawej, Sudarat Damrongwatanapokin, Dumneon Chaturawitwong Sep 2006

Surveillance Of Classical Swine Fever Virus Antibody Titers In Sows And Their Piglets Under Different Vaccination Programs, Achariya Sailasuta, Rachod Tantilertcharoen, Pornchalit Ausawacheep, Sanipa Suradhat, Praveena Kitikoon, Supot Wattanapunsak, Sawang Kesdangsakonwut, Sumittra Wattanodorn, Supasawat Buranawej, Sudarat Damrongwatanapokin, Dumneon Chaturawitwong

The Thai Journal of Veterinary Medicine

Classical swine fever virus (CSFV), serum neutralizing antibody titers of 854 sows were determined using a neutralizing peroxidase linked assay (NPLA). Blood samples were collected one-week post-partum from 1st- 6th parity sows, from 30 swine fever-free farms. The farms were divided according to their vaccination programs, 10 farms for each program. Program A sows had been vaccinated 3 weeks pre-partum (288 samples). Program B sows had been vaccinated 3 weeks post-partum (276 samples). Program C sows had been vaccinated 3 times/year (290 samples). The means log2 CSFV antibody titer of the sows in programs A, B, C were 6.591.88, 5.722.04 …


Intra And Interspecies Somatic Cell Cloning In Rabbits, Cats And Goats, Mongkol Techakumphu, Pranee Numchaisrika, Ruttachak Rungsiwiwat, Ampika Thongpakdee, Malee Apimeteetumrong, Pramuan Virutamasen Sep 2006

Intra And Interspecies Somatic Cell Cloning In Rabbits, Cats And Goats, Mongkol Techakumphu, Pranee Numchaisrika, Ruttachak Rungsiwiwat, Ampika Thongpakdee, Malee Apimeteetumrong, Pramuan Virutamasen

The Thai Journal of Veterinary Medicine

Nuclear transfer from adult somatic cells is an advanced reproductive biotechnology. This technique can be applied in animal production for producing a high genetically identical copy for endangered species conservation and for therapeutic cloning for human medicine. Our three-year preliminary study indicates the possibility of somatic cell cloning by using the rabbit as model of technical development. Later, interspecies cloning in wild cats and domestic cats was performed successfully in terms of cloned embryo production. Meanwhile, goat cloning served as the model for farm animals.


The Use Of Porcine Small Intestinal Submucosa In Ten Cases Of Canine Corneal Staphyloma, Nalinee Tuntivanich, Kumpanart Soontornwipart, Pranee Tuntivanich Sep 2006

The Use Of Porcine Small Intestinal Submucosa In Ten Cases Of Canine Corneal Staphyloma, Nalinee Tuntivanich, Kumpanart Soontornwipart, Pranee Tuntivanich

The Thai Journal of Veterinary Medicine

Porcine small intestinal submucosa biomaterial was used to repair corneal staphyloma in 10 dogs. There was no evidence of leakage of aqueous humor and graft rejection following PSIS grafting. Anterior synechia was presented in 25-50% of the corneal area. Granulation tissue vanished within 50 days of the operation. Even though scar formation and anterior synechia were observed at the end of the study, all dogs had vision.


Progesterone Receptors And Proliferation In The Bitch Uterus During Different Stages Of The Oestrous Cycle, Sayamon Srisuwatanasagul, Kongkiat Srisuwatanasagul, Sudson Sirivaidyapong Sep 2006

Progesterone Receptors And Proliferation In The Bitch Uterus During Different Stages Of The Oestrous Cycle, Sayamon Srisuwatanasagul, Kongkiat Srisuwatanasagul, Sudson Sirivaidyapong

The Thai Journal of Veterinary Medicine

Immunopresence of the progesterone receptors (PR) and proliferative activity in the bitch uterus were studied during different stages of the oestrous cycle. Uterine samples were collected at 4 different stages of the oestrous cycle; prooestrus, oestrus, dioestrus and anoestrus. Immunohistochemistry was used to evaluate the localization and to semiquantitate PR and uterine proliferation by using monoclonal antibodies to the PR and the proliferative marker, Ki-67 respectively. The results showed that PR and Ki-67 levels were highest during prooestrus and oestrus while they were low at other stages. However, a significant difference was observed only in the surface epithelium for Ki-67 …


Ultrasound Diagnosis, Phiwipha Kamonrat Sep 2006

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn Sep 2006

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn

The Thai Journal of Veterinary Medicine

No abstract provided.


Observations On The Structures Of Red Blood Cells, White Blood Cells And Platelets In Some Felis Spp. And Panthera Spp., Wimon Pothiwong, Kamol Sakulwira, Pakorn Prachammuang, Siripen Vethchagarun Sep 2006

Observations On The Structures Of Red Blood Cells, White Blood Cells And Platelets In Some Felis Spp. And Panthera Spp., Wimon Pothiwong, Kamol Sakulwira, Pakorn Prachammuang, Siripen Vethchagarun

The Thai Journal of Veterinary Medicine

Blood cells and platelets from twenty-seven animals belonging to two genera of the Family Felidae, were studied using both light and transmission electron microscopes (TEM). Generally, blood cells can be categorized into red blood cells, white blood cells and platelets. The red blood cells or erythrocytes from these species were round and had neither a nucleus nor organelles. They showed a central pale area, Heinz bodies and rouleaux formation. The white blood cells could be divided into two groups; granulocytes and agranulocytes. The granulocytes (neutrophils, eosinophils and basophils) were spherical in shape with lobulated nuclei and containing specific granules in …


Laboratory Diagnosis Of Mycoplasma Gallisepticum (Mg) Infection In Experimental Layer Chicken Receiving Mg Vaccines And Mg Organisms, Somsak Pakpinyo, Pinyo Pitayachamrat, Seubchat Saccavadit, Than Santaswang, Achara Tawatsin, Jiroj Sasipreeyajan Jun 2006

Laboratory Diagnosis Of Mycoplasma Gallisepticum (Mg) Infection In Experimental Layer Chicken Receiving Mg Vaccines And Mg Organisms, Somsak Pakpinyo, Pinyo Pitayachamrat, Seubchat Saccavadit, Than Santaswang, Achara Tawatsin, Jiroj Sasipreeyajan

The Thai Journal of Veterinary Medicine

This experiment was designed to study the results of laboratory diagnosis of Mycoplasma gallisepticum (MG) infection in layer chicken receiving MG vaccines and MG organisms. One hundred and twenty 1-day-old-malelayer chickens were raised in separate isolation rooms and equally allocated into 4 separated groups. When 4 weeks old, groups 1, 2, 3 and 4 served as a negative control, a MG commercially inactivated vaccine group, a MG commercially live vaccine group and a MG infected group, respectively. In each group, ten birds were swabbed and bled when 4, 5, 6, 7, 8, 9 and 11 weeks old. Swab samples were …


Oral Medication With Florfenicol For Black Tiger Shrimps Penaeus Monodon, Nutcharnart Tipmongkolsilp, Yanin Limpanon, Benjamas Patamalai, Pischa Lusanandana, Janenuj Wongtavatchai Jun 2006

Oral Medication With Florfenicol For Black Tiger Shrimps Penaeus Monodon, Nutcharnart Tipmongkolsilp, Yanin Limpanon, Benjamas Patamalai, Pischa Lusanandana, Janenuj Wongtavatchai

The Thai Journal of Veterinary Medicine

Florfenicol (FF) and Chloramphenicol (CAP) were tested for their in vitro antimicrobial activity against 102 Vibrio isolates from clinical cases. The Minimum Inhibitory Concentration (MIC) for both antimicrobials was ≤ 8 μg/ml suggesting that all of the tested isolates were susceptible to both antimicrobials. The observed MIC range of FF (0.5-4.0 μg/ml) was more potent than that of CAP (0.5-8.0 μg/ml). The activity of both antimicrobials was not substantially influenced by the addition of sea water (5 ppt salinity) to the test system. When considering the best type of antimicrobial for food producing animals and in vitro antivibrio activity, FF …


Hemangiosarcoma Of The Nictitating Membrane As Seen In Two Great Danes (A Case Report), Atichat Brahmasa, Nalinee Tuntivanich, Pranee Tuntivanich, Anudep Rungsipipat Jun 2006

Hemangiosarcoma Of The Nictitating Membrane As Seen In Two Great Danes (A Case Report), Atichat Brahmasa, Nalinee Tuntivanich, Pranee Tuntivanich, Anudep Rungsipipat

The Thai Journal of Veterinary Medicine

Hemangiosarcoma of the nictitating membrane of two Great Dane dogs is reported. Treatment was successfully carried out by amputation of the nictitan. No recurrence of the mass or pulmonary metastasis was evident within a period of twelve months after the treatment.


The Zoonotic Potential Of Enterocytozoon Bieneusi In Thailand, Mathirut Mungthin, Ittisak Subrungruang, Saovanee Leelayoova Jun 2006

The Zoonotic Potential Of Enterocytozoon Bieneusi In Thailand, Mathirut Mungthin, Ittisak Subrungruang, Saovanee Leelayoova

The Thai Journal of Veterinary Medicine

Enterocytozoon bieneusi is the most common microsporidia that causes disease in immunocompromised hosts, especially in AIDS patients. E. bieneusi has been reported in a wide range of animals, including domestic, wild and farm animals. Using molecular typing and phylogenetic analysis, the zoonotic potential of E. bieneusi to cause disease is indeed a threat. Transmission from animals to humans should be investigated and whether it occurs naturally, before we can address the whole subject of zoonotic transmission of E. bieneusi infection.


A Comparative Study Of Steroid Hormone Levels And Oestrogen Receptor Alpha (Erα) Between Normal Bitch Uteri And Pyometra During Dioestrus, Sayamon Srisuwatanasagul, Kongkiat Srisuwatanasagul, Sudson Sirividayapong Jun 2006

A Comparative Study Of Steroid Hormone Levels And Oestrogen Receptor Alpha (Erα) Between Normal Bitch Uteri And Pyometra During Dioestrus, Sayamon Srisuwatanasagul, Kongkiat Srisuwatanasagul, Sudson Sirividayapong

The Thai Journal of Veterinary Medicine

Ovarian steroid hormones and their receptors play an important role in both physiological as well as pathological changes seen in the uterus. For the further understanding of the mechanism of pyometra, in relation to steroid hormones and their receptors in the bitch, the difference between the expression of the oestrogen receptor alpha (ERa) between normal and pyometra bitches during dioestrus was investigated. Uterine samples were collected during dioestrus from normal and pyometra bitches. Immunohistochemistry was used to investigate the expression of ERa in the different compartments of the uterus. The results showed that the levels of steroid hormones in the …


Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Monkon Trisiriroj Jun 2006

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Monkon Trisiriroj

The Thai Journal of Veterinary Medicine

No abstract provided.


Ultrasound Diagnosis, Phiwipha Kamonrat Jun 2006

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


Malignant Catarrhal Fever In Swamp Buffaloes (Bubalus Bubalis): A Retrospective Pathological Study Of Outbreaks In Thailand, Komkrich Teankam, Rachod Tantilertcharoen, Thanasak Boonserm, Siriwat Suadsong, Wijit Banlunara Mar 2006

Malignant Catarrhal Fever In Swamp Buffaloes (Bubalus Bubalis): A Retrospective Pathological Study Of Outbreaks In Thailand, Komkrich Teankam, Rachod Tantilertcharoen, Thanasak Boonserm, Siriwat Suadsong, Wijit Banlunara

The Thai Journal of Veterinary Medicine

Malignant catarrhal fever (MCF) is a highly fatal disease of ruminants which has not been extensively studied in Thailand. The aim of this study was to describe the clinical signs and pathological findings of MCFaffected buffaloes (n = 13), in the Nakhonpathom province of Thailand. All cases were collected between May 1996 and August 2000. Before the 1998 outbreak, four clinically normal sheep were moved into a pen adjacent to the buffaloes’ pens. The animals developed clinical signs of severe depression, anorexia, high fever, hypersalivation, and corneal opacity-associated excessive lacrimation. Necropsy revealed severe haemorrhages in various organs including the oropharynx, …


The Variations And Patterns Of Renal Arteries In Dogs, Kriengyot Sajjarengpong, Adisorn Adirektaworn Mar 2006

The Variations And Patterns Of Renal Arteries In Dogs, Kriengyot Sajjarengpong, Adisorn Adirektaworn

The Thai Journal of Veterinary Medicine

288 kidneys complete with renal arteries and abdominal aorta, were collected from 144 dogs (75 males and 69 females), dissected and studied according to the number, branch, length and position of the renal arteries bifurcation. The positions of the left and right renal arteries were also observed. Double renal arteries were noted in 14 of 144 dogs (9.72%) but only on the left side. Single renal arteries had one to six branches. Most kidneys had 2 (39.42%) or 3 (33.58%) branches. The bifurcation mostly occurred in the middle of the renal artery (60.61%). Only ten kidneys (3.65%) were supplied by …


Ultrasound Diagnosis, Phiwipha Kamonrat Mar 2006

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


Proceedings Ann. Con. Vet. Sci. Chula. Meeting/27-28 April, 2006., Ornsiri Cheunsuang Mar 2006

Proceedings Ann. Con. Vet. Sci. Chula. Meeting/27-28 April, 2006., Ornsiri Cheunsuang

The Thai Journal of Veterinary Medicine

No abstract provided.


Relationships Between The Immune System And Stress Reactivity In Pig: Visualizing The Immuno-Neuroendocrine Framework In Action, Sanipa Suradhat Mar 2006

Relationships Between The Immune System And Stress Reactivity In Pig: Visualizing The Immuno-Neuroendocrine Framework In Action, Sanipa Suradhat

The Thai Journal of Veterinary Medicine

Activation of the immune system is regulated through several mechanisms, within the system itself and by other body systems, in order to maintain disease resistance, and prevent immune-mediated disorders within the host. It is well accepted that the immunological, physiological, behavioral, and psychological state of an animal are closely linked and the factors affecting one feature of the network can influence the rest of the system.In recent years, our understanding of the interactions between the neuroendocrine network (i.e. the hypothalamicp tuitary- adrenal axis, HPA) and immune-mediated inflammatory reactions has expanded enormously. This article outlines the nature of the immuno-neuroendocrine network …