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Pka-Mediated Erk1/2 Inactivation And Hsp70 Gene Expression Following Exercise, C.W. Melling, Matthew Krause, Earl Noble
Pka-Mediated Erk1/2 Inactivation And Hsp70 Gene Expression Following Exercise, C.W. Melling, Matthew Krause, Earl Noble
Jamie Melling
Exercise induces the expression of the cardioprotective protein, Hsp70, through the activation of its transcription factor HSF1. Recently, we reported that administration of a protein kinase A (PKA) inhibitor suppressed exercise-induced hsp70 gene expression, suggesting a role for PKA in the regulation of HSF1 activation in vivo. While the mechanism by which PKA regulates HSF1 is unclear, studies in vitro have reported that HSF1 is phosphorylated on two serine residues by mitogen activated protein kinases (MAPKs); ERK1/2 (ser307) and JNK/SAPK (ser363). As PKA is a regulator of these protein kinases, the current study examined the role of PKA in their …
Lactobacillus Vaginal Microbiota Of Women Attending A Reproductive Health Care Service In Benin City, Nigeria., Kingsley Anukam, Emmanuel Osazuwa, Ijeoma Ahonkhai, Gregor Reid
Lactobacillus Vaginal Microbiota Of Women Attending A Reproductive Health Care Service In Benin City, Nigeria., Kingsley Anukam, Emmanuel Osazuwa, Ijeoma Ahonkhai, Gregor Reid
Kingsley C Anukam
OBJECTIVE: The objective of this study was to determine whether Lactobacillus species found in African women differ substantially to those of Caucasian decent, described in previous studies. The vaginal microbiota play an important role in female health, and when the naturally dominant lactobacilli are displaced resulting in bacterial vaginosis (BV), the host is more at risk of acquiring sexually transmitted diseases, including HIV. METHODS: Vaginal samples were collected from 241 healthy, pre-menopausal Nigerian women, which were then Gram stained for Nugent scoring. Microbial DNA was extracted, amplified using PCR and Lactobacillus primers, and processed by Denaturing Gradient Gel Electrophoresis (DGGE). …