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Full-Text Articles in Medicine and Health Sciences
Protein Kinase C Activity In Mouse Eggs Regulates Gamete Membrane Interaction, Hiroto Akabane
Protein Kinase C Activity In Mouse Eggs Regulates Gamete Membrane Interaction, Hiroto Akabane
Theses, Dissertations and Capstones
Every mammalian life develops from one cell after fertilization of an egg by the sperm. The molecular pathways governing this event are still poorly understood. Numerous reports indicate that mammalian eggs highly express various protein kinase C (PKC) isoforms. Accordingly, we hypothesize that PKC activity in the egg plays an important role during egg-sperm membrane binding and fusion. In this study, we tested our hypothesis in mouse gametes using two types of PKC inhibitors (calphostin c and staurosporine) and the typical PKC activator, phorbol ester 12-tetradecanoylphorbol-13 acetate (PMA). After treatment with the individual drug, eggs were inseminated with sperm. The …
Regulation Of Biofilm Formation Of Pseudomonas Aeruginosa, Nathaniel Edwards Head
Regulation Of Biofilm Formation Of Pseudomonas Aeruginosa, Nathaniel Edwards Head
Theses, Dissertations and Capstones
Cystic fibrosis (CF) is the most common, autosomal recessive lethal genetic disease in the Caucasian population, resulting from a malfunctioned cystic fibrosis transmembrane conductance regulator (CFTR) and leading to bacterial lung infections. P. aeruginosa, an opportunistic pathogen, establishes a chronic infection in CF with a phenotype of overproduction of an exopolysaccharide (alginate) due to host-directed mutagenesis. While free-floating planktonic bacteria can be properly cleared from the CF lung, P. aeruginosa, along with alginate production, establishes an infection in the form of a biofilm which supports its survival in nature and in vivo. As a result, genomic structure, …