Medicine and Health Sciences Commons^™

Antimicrobial And Spermicidal Activity Of Hydrogen Peroxide And Nonoxynol-9 Based Gel Preparations, Paul E. Grimmett

Theses, Dissertations and Capstones

Eighty gel formulations were prepared using five buffer systems (0.01M citrate, 0.02M citrate, 0.01M tartrate, 0.02M tartrate, and 0.01M citrate/0.01M tartrate), four hydrogen peroxide (H2O2) concentrations (0%, 0.3%, 0.9%, and 1.2%), and four nonoxynol-9 (N-9) concentrations (0%, 1%, 1.5%, and 2%). The eighty gel formulations were then tested for physio-chemical properties (viscosity and pH), antimicrobial effectiveness (Minimum Inhibitory Concentration Assay) against Candida albicans, Escherichia coli, and Streptococcus agalactiae, and finally, spermicidal effectiveness (Sander Cramer Assay). The gel formulation that contained the best combination of physio-chemical, antimicrobial, and spermicidal properties was a 0.01M citrate buffered hydrogel, which contained 0.9% H2O2 and …

Factors That Most Influence Job Satisfaction Among Cardiac Nurses In An Acute Care Setting, Lashonda Leigh Bare

Theses, Dissertations and Capstones

The purpose of this study is to determine cardiac nurses’ level of job satisfaction through an examination of the relationship between Herzberg’s motivation and hygiene factors and job satisfaction. The study used a non-experimental, quantitative design with a theoretical framework based on Herzberg’s Motivation Versus Hygiene theory. The independent variables were motivation and hygiene factors and the dependent variable was nurse job satisfaction. The Work Quality Index and a demographic survey were given to approximately 32 registered nurses at a medical center in southwestern, West Virginia. The data was collected over three weeks and was analyzed using descriptive and correlational …

Renal Basolateral Transport Of Glucuronides And Other Organic Anions In Rat In Vitro Models, Sarah L. Miles

Theses, Dissertations and Capstones

Glucuronidation is a common Phase II biotransformation reaction that increases the hydrophilicity, and thus elimination, of toxins, xenobiotics, and endogenous compounds. Previous studies suggest that the kidney can secrete glucuronide conjugates, but the renal transport mechanisms for glucuronide secretion have not been determined. Based on the chemical nature of glucuronide metabolites, it is hypothesized that organic anion transporter (OAT) proteins along the basolateral membrane of the renal proximal tubule promote renal accumulation of glucuronide conjugates. The purpose of this study was to develop a rat renal proximal tubule model which demonstrates OAT activity and by which the contribution of OAT …

Hk-2 Cells As A Human Model Of Glucuronide Transport, Eliza E. Robertson

Theses, Dissertations and Capstones

Glucuronidation is primarily a pathway of detoxification in most species, but many glucuronide conjugates are associated with toxicity. Numerous drugs are excreted in the urine as glucuronide conjugates. Being organic anions, it is likely that glucuronides are secreted into the urine by organic anion transporters found in renal proximal tubule cells (PTCs). Some drugs that are metabolized by glucuronidation have been shown to cause renal toxicity, yet little is known about the renal handling of glucuronide conjugates. It is hypothesized that glucuronides are transported into renal PTCs by an organic anion transporter (OAT) on the basolateral membrane of the cell. …

The Genetic And Molecular Characterization Of The Polycystic Kidney Disease-Causing Mouse Gene Bicc1, Sarah J. Price

Theses, Dissertations and Capstones

Polycystic kidney disease (PKD) is one of the most common hereditary diseases and is characterized by progressive cyst formation, substantial renal enlargement, and frequently, progression to end-stage renal disease. One way to learn more about the etiology of this disease is to study mouse models that imitate the human situation. The juvenile congenital polycystic kidney disease (jcpk) gene on mouse Chromosome 10 has been found to cause a severe, early onset form of PKD when inherited in an autosomal recessive manner (Flaherty et al., 1995). Previous genetic studies mapped the jcpk locus to a 1 cM region on mouse Chromosome …

The Effects Of A Persuasive Presentation And Group Discussion On Parental Attitude In A Youth Sports Camp Parent Conference, Nicholas Charles Mirabile

Theses, Dissertations and Capstones

The general purpose of this study was to increase parental support for inclusion of services for children with special needs. A persuasive presentation and group discussion were conducted at a parent conference at a youth sports camp

The presenter was a social worker who has experience working with children with special needs. The presenter developed the presentation and helped guide the discussion.

Anonymous pre-test and post-test surveys of parental attitude toward the presentation issue were taken. From these surveys, the effects of level of involvement and level of knowledge on level of importance were assessed.

Attitude change occurred in this …

Cytoanalysis Of Pancreatic B-Cells: Using An Avian Model, Mammalian Tissue Culture And Implications Of Antisense Oligonucleotides Transfection, Ayman Salah-El-Deen Amer

Theses, Dissertations and Capstones

Calbindin-D_28k (CaBP_28K) is a vitamin D-dependent calcium-binding protein that may alter intracellular calcium ion levels, [Ca²⁺]i. This dissertation describes experiments done to gain an understanding of the potential role of CaBP_28k in pancreatic B-cells in control of insulin secretion. The localization of CaBP_28k and insulin in chicken pancreas are shown in Chapter 1. CaBP_28k expression was found to be highest in ventral and dorsal lobes and lowest in splenic lobe. Insulin concentrations were distributed similarly among these lobes. Confocal microscopic studies demonstrated colocalization of insulin and CaBP_28k in Bcells. These findings …

The Use Of Ergogenic Aids Among High School Athletes In Eastern Kentucky, John Robert Brandenburg

Theses, Dissertations and Capstones

A fifteen-question survey was developed to study the use of over-the counter nutrition supplements by high school athletes in eastern Kentucky. Athletic trainers from four local high schools were given one hundred permission forms and surveys. After two weeks only a small number (n=25) completed the requirements to participate in the study. The twenty-five participants consisted of male (n=15) and female (n=10). The athletes with the highest frequency of supplement use were football players (67.7%). The supplements with the highest use were protein powders (n=13) and creatine monohydrate (n=9).

The Relationship Among The Types, Frequency And Resolution Of Moral Conflicts Perceived By Nurses In West Virginia And Their Educational Preparation, Educational Needs, And Selected Demographics, Nancy K. Dunn

Theses, Dissertations and Capstones

Technological changes, interpersonal relationships, and scarce resources have created a complex environment for health professionals. Nurses, as the largest group of health care providers, are confronted with moral issues when dealing with managed care policies, end-of-life care and workplace or institutional issues. Moral issues are expressed as moral distress, which has been linked to decreased satisfaction, termination of employment and leaving the profession of nursing altogether. Moral distress, therefore, decreases the number of nurses and contributes to the critical nursing shortage. The purpose of this study was to examine the relationships, if any, among the types, frequency and resolution of …

Smoking Cessation Education Needs As Reported By Current Smokers, Former Smokers, And Nurses, Ana Grynwald

Theses, Dissertations and Capstones

Purpose: The purpose of this study was to determine if there is a difference in the importance of selected smoking cessation information to current smokers, former smokers, and nurses in the Appalachian population. There were no instruments found in the literature to assess the perceptions of educational content for smoking cessation, therefore, a Smoking Cessation Education (SCE) survey was developed to measure the perceptions. The conceptual framework for this study was based on the Health Belief Model developed by psychologists Drs. Godfrey M. Hochbaum, S. Stephen Kegeles, Howard Leventhal, and Irwin M. Rosenstock..

Design: The SCE is a ten question …

Roles Of Pkc Isozymes In Retinoic Acid-Induced B16 Mouse Melanoma Cell Growth Inhibition And Differentiation, Jianming Han

Theses, Dissertations and Capstones

Protein Kinase C (PKC) has been implicated in many cellular responses. Of the 11 isotypes in this family, seven are expressed in B16 mouse melanoma cells − PKCα, PKCδ, PKCε, PKCθ, PKCλ, PKCζ, and PKCµ.

Treatment of B16 cells with retinoic acid (RA) results in growth inhibition and differentiation. Simultaneously, RA induces a 6-8 fold increase of PKC α protein level, but only about 2-fold increase of PKC enzyme activity. The over- expression of wild type PKCα in B16 cells mimicked the action of RA. The over- expression of enzymatically inactive PKCα, either open or closed conformation, also inhibited B16 …

Docosahexaenoic Acid Modulates Class I Major Histocompatibility Complex Protein Function, Kaleb Marie Hypes

Theses, Dissertations and Capstones

Fatty acids influence immune responses, but how this occurs at the molecular level is not understood. Class I major histocompatibility complex molecules (MHC I), transmembrane proteins required for antigen presentation to T cell receptors, each consist of a 45kDa α chain noncovalently associated with a 12-kDa β₂-microglobulin (β₂m) molecule. Docosahexaenoic acid’s (DHA) effect on the binding of β₂m and anti-MHC I monoclonal antibodies (Mab) was measured. EL4 and RMA-S cells were cultured 48 hours with 0-45µM DHA or oleic acid. Murine to human β₂m exchange and Mab binding were assessed by fluorescence …