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Improved Methods For The Isolation And Characterization Of Flavobacterium Columnare, Bradley Donovan Farmer
Improved Methods For The Isolation And Characterization Of Flavobacterium Columnare, Bradley Donovan Farmer
LSU Master's Theses
Columnaris disease, caused by the bacterium Flavobacterium columnare, is an economically significant problem in many warmwater fish species. Difficulties encountered in the isolation and culture of F. columnare have been an impediment to research on the organism and the disease it causes. The goal of this study was to improve the methods for isolation, culture, identification and maintenance of F. columnare. Following the evaluation of different culture media selective cytophaga agar was determined to be the optimum isolation medium, Flavobacterum columnare growth medium proved to be the optimum culture medium, and tryptone yeast extract agar with increased moisture was best …