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Ultrastructural Changes In Porcine Oocytes After Ultrarapid Freezing, Adisorn Adirekthaworn, Kriengyot Sajjarengpong, Siripen Vethchagarun, Wimon Pothiwong, Mongkol Techakumphu Dec 2004

Ultrastructural Changes In Porcine Oocytes After Ultrarapid Freezing, Adisorn Adirekthaworn, Kriengyot Sajjarengpong, Siripen Vethchagarun, Wimon Pothiwong, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

The objective of the present study was to investigate the ultrastructural changes seen in frozen porcine oocytes at the germinal vesicle and during in vitro maturation when using an ultrarapid freezing technique. The morphological changes were assessed by transmission electron microscopy (TEM). Immature oocytes were collected from pig ovaries at a local slaughter house in Nakorn Prathom province. Some oocytes were matured in vitro for 44 hours at 39oC, 5% CO2 with high humidity. All oocytes were divided into four groups; immature (control group), the in vitro maturation group (control group), frozen immature and frozen mature oocytes. Electron microscopy was …


Melan A, Protein Expression As A Diagnostic And Prognostic Factor In Canine Oral Melanomas, Anudep Rungsipipat, Chanin Kulprawit Dec 2004

Melan A, Protein Expression As A Diagnostic And Prognostic Factor In Canine Oral Melanomas, Anudep Rungsipipat, Chanin Kulprawit

The Thai Journal of Veterinary Medicine

The purpose of this paper is to evaluate the relationship between Melan A protein expression, histopathological appearance, clinical data and proliferative marker indices for the diagnosis and prognosis of canine oral melanomas. A retrospective study was performed on biopsy specimens of 65 dogs submitted during the years of 2000 to 2003 to the Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University. Our results revealed that Melan A protein expression was detected in 50.77% (33/65) malignant melanomas. The results did not show any significant differences between Melan A protein expression and histopathological classification, clinical data, average AgNORs and PCNA indices. …


The Prevalence And Treatment Of Eyelid Neoplasms In Dogs : 69 Cases (2000-2003), Nalinee Tuntivanich, Pranee Tuntivanich, Santi Wongaumnuaykul, Anudep Rungsipipat Dec 2004

The Prevalence And Treatment Of Eyelid Neoplasms In Dogs : 69 Cases (2000-2003), Nalinee Tuntivanich, Pranee Tuntivanich, Santi Wongaumnuaykul, Anudep Rungsipipat

The Thai Journal of Veterinary Medicine

The prevalence and treatment of 69 eyelid masses in dogs at the Ophthalmology clinic, Department of Surgery, Small Animal Hospital, Chulalongkorn University, between November 2000 and November 2003, in relation to breed, sex, age, location, tumor size, histopathological diagnosis and surgical treatment is reported. The average age of the affected dogs was 7.85 years. Mixed breeds, males and the upper eyelid were at a higher risk. The average size of the mass was 6.49 mm. Benign tumors (88.41%) were more frequent than malignant ones (11.59%). Sebaceous gland tumors were more frequently encounted (79.7%) than malignant tumors, malignant melanomas, squamous cell …


Ultrasound Diagnosis, Phiwipha Kamonrat Dec 2004

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


Some Effects Of Organic Acids On The Control Of Salmonella In Broiler Chickens, Indhira Kramomtong, Waree Niyomthum, Nuttee Am-In, Piriya Uttama, Vichuda Quanjai Dec 2004

Some Effects Of Organic Acids On The Control Of Salmonella In Broiler Chickens, Indhira Kramomtong, Waree Niyomthum, Nuttee Am-In, Piriya Uttama, Vichuda Quanjai

The Thai Journal of Veterinary Medicine

Three hundred and sixty day-old chicks were used in this study to investigate the effects of organic acids on the control of Salmonella. The chicks were divided into uninoculated and inoculated groups, 180 in each. Both groups were subdivided into 3 further groups of 60 chicks each. The uninoculated, control group comprised of, group 1 : given diluted organic acids 1:1000 via the drinking water, group 2 : given organic acids diluted at 1:2000 and group 3 : given water alone. Each bird in the three inoculated subgroups 4, 5 and 6 were orally dosed with 0.3 ml of brain …


Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn Dec 2004

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn

The Thai Journal of Veterinary Medicine

No abstract provided.


The Expression Of C-Erbb2 Oncogene Protein As A Prognostic Factor In Feline Mammary Tumors, Mataya Taweechart, On-Uma Sirayayon, Grisnarong Wongbandue, Supradit Wangnaitham, Achariya Sailasuta, Sudson Sirividhayapong, Anudep Rungsipipat Dec 2004

The Expression Of C-Erbb2 Oncogene Protein As A Prognostic Factor In Feline Mammary Tumors, Mataya Taweechart, On-Uma Sirayayon, Grisnarong Wongbandue, Supradit Wangnaitham, Achariya Sailasuta, Sudson Sirividhayapong, Anudep Rungsipipat

The Thai Journal of Veterinary Medicine

The study looked at the relationship between the expression of c-erbB2 oncogene protein, histological types, PCNA and AgNORs indices, in feline mammary tumors. Sixty, surgically resected, mammary tumors from cats were collected and histological processed. The expression of c-erbB2 oncogene protein was detected by immunohistochemistry and was seen in benign hyperplasia, adenosis and malignant groups. The c-erbB2 oncogene protein expression in malignant, feline mammary tumors was 41.67% (25/60) which could be divided into simple tubular ACC, 25% (6/24), simple papillary ACC, 78.6% (11/14), solid carcinoma, 26.7% (4/15) and cribiform carcinoma, 57.14% (4/7). There were statistically significantly differences (p<0.05) in the expression of c-erbB2 oncogene protein between simple tubular ACC, compared with simple papillary ACC and solid carcinomas. The expression of c-erbB2 oncogene protein in specimens, classified by histological malignancy grades revealed, grade I, 42.11% and grade II, 41.46% but there was no statistically significant difference between them. An evaluation of a proliferative marker shown by a mean (±SD) of PCNA, was 34.63±23.87 and the mean (±SD) of AgNORs was 1.23±0.18. There was no significant difference between the PCNA and AgNORs index of both the histological types and the histological malignancy grades. However, a statistical difference of mean PCNA and AgNORs indices was noted in both c-erbB2 positive and c-erbB2 negative groups. The mean PCNA indices and AgNORs indices in c-erbB2 positive samples were 26.52±18.68 and 1.12±0.27 respectively, while the c-erbB2 negative ones were 39.43±26.24 and 1.28±0.19 respectively. In conclusion, c-erbB2 oncogene protein expression is considered to be an effective prognostic factor for adenocarcinoma subtypes of feline mammary tumors as it is in human and canine cases. However, integration of all the parameters (c-erbB2 oncogene protein, PCNA index, AgNORs index, histological types and clinical data) may achieve a more precise prognosis in feline, mammary tumor cases.


The Relationship Between Agnors And Pcna Indices, Histopathological Appearance And Clinical Factors, As Prognostic Factor For Canine Oral Melanoma, Anudep Rungsipipat, Siriwalee Ngow, Nipaporn Pattanathaiyanom, Nipaporn Pattanathaiyanom, Porntipa Lertrattanachaikul, Khanchit Pongpech, Chanin Kulprawit Dec 2004

The Relationship Between Agnors And Pcna Indices, Histopathological Appearance And Clinical Factors, As Prognostic Factor For Canine Oral Melanoma, Anudep Rungsipipat, Siriwalee Ngow, Nipaporn Pattanathaiyanom, Nipaporn Pattanathaiyanom, Porntipa Lertrattanachaikul, Khanchit Pongpech, Chanin Kulprawit

The Thai Journal of Veterinary Medicine

The purpose of this research was to evaluate the relationship between histopathological appearance and clinical data, Argyrophilic Nucleolar Organizer Regions (AgNORs) and Proliferative Cell Nuclear Antigen (PCNA) indices in canine oral melanoma. A retrospective study was performed on biopsy specimens from 67 dogs submitted over the year 2000 to 2003 to the Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University. Our results showed that the average AgNORs index did not show any significant differences with histopathological characteristics and Masson-Fontana Silver (MFS) stain but the AgNORs index showed significant differences when compared with histological grading and tumor diameter size (p < 0.05). The average PCNA index did not show significant differences with histopathological characteristics, grading, and MFS stain but did not show significant differences with the sex of affected dogs and tumor diameter size (p < 0.05). AgNORs and PCNA indices might be useful as a prognostic factor for oral melanoma in dogs


In Vitro Effects Of Sodium Hypochlorite, Trisodium Phosphate And Organic Acids On The Decontamination Of Salmonella Enterica Serovar Enteritidis On Chicken Skin, Rungtip Chuanchuen, Chailai Koowatananukul, Valasinee Rugkhaw, Thanis Damrongwatanapokin Dec 2004

In Vitro Effects Of Sodium Hypochlorite, Trisodium Phosphate And Organic Acids On The Decontamination Of Salmonella Enterica Serovar Enteritidis On Chicken Skin, Rungtip Chuanchuen, Chailai Koowatananukul, Valasinee Rugkhaw, Thanis Damrongwatanapokin

The Thai Journal of Veterinary Medicine

This study was conducted to evaluate the effectiveness of five chemical disinfectants, including sodium hypochlorite (CL), trisodium phosphate (TSP) and organic acids; acetic acid (AA), citric acid (CA) and lactic acid (LA), in reducing of the contamination level of Salmonella enterica Serovar Enteritidis (S. Enteritidis) on chicken skin. Chicken drumsticks were artificially contaminated with the kanamycin resistant S. Enteritidis (SEnKmr) to obtain 105-106 CFU/cm2. The five disinfectants used were: CL; 25, 50 and 100 ppm, TSP; 4, 8 and 12%, AA, LA and CA; 0.6, 1.2 and 2.4%. The disinfectants were applied by either dipping for 1, 3 and 5 …


A Comparative Study On The Isolation Of Ureaplasma Diversum From The Vulvae Of The Cattle, Using Three Different Culture Media, Junpen Suwimonteerabutr, Prachin Virakul Dec 2004

A Comparative Study On The Isolation Of Ureaplasma Diversum From The Vulvae Of The Cattle, Using Three Different Culture Media, Junpen Suwimonteerabutr, Prachin Virakul

The Thai Journal of Veterinary Medicine

The objective of the present study was to investigate the optimum culture media for Ureaplasma diversum. The study used 3 culture media, TR broth and differential A 7 agar (1), Boston broth and differential A7 agar (2) and differential A7 agar (3). The samples were collected from the vulvae of 15 cows that had a reddish, mucosal, vulva lesion and 15 cows without the lesion. A liquid culture media was prepared using the serial, tenfold, dilutionmethod to avoid contamination from other microflora. The type of U. diversum was determined using A7 agar mixed with manganese sulfate. Positive results were observed …


Multidrug Efflux Systems (Mes): A Mechanism Of Resistance To Multidrug In Bacteria, Rungtip Chuanchuen, Herbert P. Schweizer Dec 2004

Multidrug Efflux Systems (Mes): A Mechanism Of Resistance To Multidrug In Bacteria, Rungtip Chuanchuen, Herbert P. Schweizer

The Thai Journal of Veterinary Medicine

Bacterial antibiotic resistance is a growing problem with a global dimension. While the mechanisms of resistance vary from agent to agent, most of them are agent specific, providing resistance to a single or a class of anitimicrobials. Multidrug efflux systems have currently emerged as a predominant cause of antimicrobial resistance across a broad spectrum of structurally - unrelated antimicrobials. Efflux-mediated resistance is promoted by the exclusion of agents from the systems, resulting in reduced intracellular concentration of drugs. MES occur in many Gram-positive and Gram-negative bacteria, including medically important pathogens, therefore they are of significant concern. Expression of the systems …


What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthateph Dec 2004

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthateph

The Thai Journal of Veterinary Medicine

No abstract provided.


The Results Of Using Pumices As A Bedding In Broiler Chicken House, Somsak Pakpinyo, Boonrit Thongsong, Thawat Lekdumrongsak, Somporn Wangsoongnoen, Suthep Ruangwises Dec 2004

The Results Of Using Pumices As A Bedding In Broiler Chicken House, Somsak Pakpinyo, Boonrit Thongsong, Thawat Lekdumrongsak, Somporn Wangsoongnoen, Suthep Ruangwises

The Thai Journal of Veterinary Medicine

The objective of this experiment was to compare the rice hull and pumice litter when used as bedding in a broiler chicken house. Seven hundred and fourteen 1-day-old, broiler chicks were divided equally into 2 groups. Group 1 raised on rice hulls and group 2 on pumice litter. Each group had 3 replicates, 119 birds in each. The average body weight gain and the feed intake during days 1-42 were observed in order to calculate the feed conversion rate (FCR). At 16, 28, and 42 days, 10 birds from each replicate were bled for their antibody titer responses to Newcastle …


The Characterization Of Clays And Cetylpyridiniumexchanged Clays For Their Ability To Adsorb Zearalenone, Komkrich Pimpukdee, Bundit Tengjaroenkul, Prapansak Chaveerach, Benjamas Mhosatanun Dec 2004

The Characterization Of Clays And Cetylpyridiniumexchanged Clays For Their Ability To Adsorb Zearalenone, Komkrich Pimpukdee, Bundit Tengjaroenkul, Prapansak Chaveerach, Benjamas Mhosatanun

The Thai Journal of Veterinary Medicine

The adsorption of zearalenone (ZEN) onto Ca-montmorillonite (STx-1), Na-montmorillonite (SWy-2), acid-activated montmorillonite (AAM), clinoptilolite and cetylpyridinium (CP) exchanged clays was studied. The results indicated that CP-exchanged clays were more effective than unmodified base clays in adsorbing ZEN, i. e. STx-1 (7.4±2.3 mg/g) < clinoptilolite (7.6±1.1 mg/g) < AAM (10.0±0.0 mg/g) < SWy-2 (11.9±0.4 mg/g), whereas the extent of ZEN adsorption into modified clay products did not follow the order seen in the based clays. The adsorption efficiency of CP-exchanged clays is in the order of CP*STx-1 (56.9±1.1 mg/g) = CP*AAM (56.9±1.8 mg/g) > CP*SWy-2 (47.9±2.6 mg/g) > CP*clinoptilolite (15.5±1.1 mg/g). Three CP-exchanged clays were further characterized by isotherm studies. The results indicated that the isotherm shapes of CP*exchanged clays exhibited a S-shape. The isotherm plot is concave up to the inflection point and then plateaus, which suggests multiple, specific binding sites and saturation at each site. It is important to rigorously test …


Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn Jun 2004

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn

The Thai Journal of Veterinary Medicine

No abstract provided.


Physiological Reactions Of Poultry To Heat Stress And Methods To Reduce Its Effects On Poultry Production, Narongsak Chaiyabutr Jun 2004

Physiological Reactions Of Poultry To Heat Stress And Methods To Reduce Its Effects On Poultry Production, Narongsak Chaiyabutr

The Thai Journal of Veterinary Medicine

Thermal environment is regarded as the prime factor governing the production because, both the feed consumption and heat production are related closely to the thermal environment. Therefore, heat stress is caused by a combination of environmental and animal factors that result in reduced performance as the animal attempts to cool itself. In this review, the physiological reactions of bodily functions during heat exposure in poultry are described. Thermal panting, the marked response of poultry to heat stress, is compensatory behavior to permit a high rate of evaporative cooling from the respiratory tract. Cardiorespiratory responses to thermal stress show different patterns …


The Estimation Of Glomerular Filtration Rates Using Endogenous Creatinine Clearance In Nine Dogs, Chollada Buranakarl, Wanchai Yenpetch, Siram Suvarnavibhaja Jun 2004

The Estimation Of Glomerular Filtration Rates Using Endogenous Creatinine Clearance In Nine Dogs, Chollada Buranakarl, Wanchai Yenpetch, Siram Suvarnavibhaja

The Thai Journal of Veterinary Medicine

The objective of this study was to estimate the glomerular filtration rates of normal dogs using the clearance of endogenous creatinine, and comparing it to the clearance of exogenous creatinine. Nine healthy dogs, of both sexes, were used in this study. The results showed a significant relationship, with a correlation coefficient = 0.714 (p<0.05). The regression line was an endogenous creatinine clearance = (-0.803 + 0.677) x exogenous creatinine clearance. The use of endogenous creatinine clearance is a simple and convenient method without the need to administer any substances and can be used to evaluate renal function in clinical practice.


The Effect Of Feeding Extra Colostrum, Obtaining Using An Electric Sow Milking Machine On Farrowing Sows, On The Survival Rate Of Small-Sized Piglets, Vivat Chavananikul, Nalinee Imboonta, Sahas Nuchanart Jun 2004

The Effect Of Feeding Extra Colostrum, Obtaining Using An Electric Sow Milking Machine On Farrowing Sows, On The Survival Rate Of Small-Sized Piglets, Vivat Chavananikul, Nalinee Imboonta, Sahas Nuchanart

The Thai Journal of Veterinary Medicine

The study was conducted in 2 parts. The first was the development of a milking machine for sows, composed of a vacuum generator providing 33-38 cmHg, a pulse rate of 30-45/mins and a set of 2 teat cups to fit the sows teats. The results from 34 milkings at farm 1 and 28 milkings at farm 2 showed that the amount of colostrum obtained was 96.77±38.63 ml and 114.93±75.09 ml, respectively, taking an average of 19 mins/milking with no damage to the udder. The second part of the study was to manually feed 9 ml of the colostrum, obtained from …


Ultrasound Diagnosis, Phiwipha Kamonrat Jun 2004

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


The Detection Of Ifn-Γ Positive Cells In The Lymphoid Tissues Of Prrsv-Infected Pigs Using Different Strains, For Infection, Tuddow Thaiwong, Chanis Leetheraprasert, Chaiwat Winyoonunthakul, Anudep Rungsipipat, Supradit Wangnaitham, Roongroje Thanawongnuwech Jun 2004

The Detection Of Ifn-Γ Positive Cells In The Lymphoid Tissues Of Prrsv-Infected Pigs Using Different Strains, For Infection, Tuddow Thaiwong, Chanis Leetheraprasert, Chaiwat Winyoonunthakul, Anudep Rungsipipat, Supradit Wangnaitham, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

Archived paraffin-embedded lymphoid tissue samples from seventy five experimental PRRSV-infected pigs were examined for PRRSV antigen and IFN-γ positive cells, using immunohistochemistry. Lymphoid tissues (5 pigs/group) including spleen, tracheobronchial lymph nodes and tonsil came from negative control pigs (group 1), low virulence strain of PRRSV (RespPRRS/ReproTM, modified-live virus vaccine) infected pigs (group 2) and high virulence strain of PRRSV (VR-2385) infected pigs(group 3). They were necropsied at 3, 7,10, 14 or 28 days post-inoculation (DPI). According to the study, microscopic lesions in the lymphoid tissues from pigs infected with the high virulence strain had more severe lesions, characterized by severe …


Proceedings Of The 6th Chulalongkorn University Veterinary Annual Conference 20-21 May 2004, Piyarat Chansiripornchai Jun 2004

Proceedings Of The 6th Chulalongkorn University Veterinary Annual Conference 20-21 May 2004, Piyarat Chansiripornchai

The Thai Journal of Veterinary Medicine

No abstract provided.


The Function Of The Oviduct In Relation To Sperm Transport And Capacitation In Swine, Paisan Tienthai Jun 2004

The Function Of The Oviduct In Relation To Sperm Transport And Capacitation In Swine, Paisan Tienthai

The Thai Journal of Veterinary Medicine

In the pig, sperm transport is regulated, to facilitate normal fertilization of the ovulated oocytes, by the oviduct which contains a particular segment, the sperm reservoir. The main function of the sperm reservoir is to ensure that stored spermatozoa have maintained viability and fertilizing capacity. The process of sperm capacitation is delayed in the sperm reservoir, during the lengthy pre-ovulatory estrus by interactions between the spermatozoa, the oviduct epithelium and secretion products. Sperm capacitation seems to be facilitated by the tubal fluid in the upper segment of the oviduct releasing sperm from the reservoir in relation to the time of …


Pcr-Rflp Analysis In An Open Reading Frame 5 Of Variants Of Prrsv Isolated In Thailand, Nusara Punprapa, Anudep Rungsipipat, Amornrat Tatsanakit, Piya Wongyanin, Roongroje Thanawongnuwech Jun 2004

Pcr-Rflp Analysis In An Open Reading Frame 5 Of Variants Of Prrsv Isolated In Thailand, Nusara Punprapa, Anudep Rungsipipat, Amornrat Tatsanakit, Piya Wongyanin, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

Ten selected Thai isolates of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) were amplified in an open reading frame 5 by a reverse, transcriptase, polymerase chain reaction (RT-PCR) and cut with restriction enzymes: MluI, HincII, SacII and HaeIII for the US genotype and PstI, HaeII and ClaI for the EU genotype. None of the field isolates had a similar cutting pattern when compared to modified live virus vaccines, however, several cutting patterns were obtained from this study. The results suggested that genetic variation was present among the Thai isolates, even within the same genotype. The variations may be the result …


The Effect Of Parity Number, Body Condition Score, Backfat Thickness And Weaning To Oestrus Interval On The Time Of Ovulation In Sows, Woravit Anuvongnukroh, Chaowaphan Yinharnmingmongkol, Sutthatip Punieam, Padet Tummaruk, Annop Kunavongkrit Jun 2004

The Effect Of Parity Number, Body Condition Score, Backfat Thickness And Weaning To Oestrus Interval On The Time Of Ovulation In Sows, Woravit Anuvongnukroh, Chaowaphan Yinharnmingmongkol, Sutthatip Punieam, Padet Tummaruk, Annop Kunavongkrit

The Thai Journal of Veterinary Medicine

The present study was performed to study the time of ovulation in 53 weaned sows, by using real time B-mode ultrasonography and to study the influence of parity, body condition score, backfat thickness and weaning-to-oestrus interval on the time of ovulation. The study revealed that ultrasound B mode successfully determined the time of ovulation in 37 sows. Parity number, weaning-to-oestrus interval and oestrus duration influenced the ovulation time, whereas backfat thickness and body condition score was not correlated with either the ovulation time or the duration of oestrus.


The Quantitative Analysis Of Oxytetracycline In Black Tiger Shrimps (Penaeus Monodon) Muscle Tissue And Hepatopancreas, After Oral Medication, Mathu Bumrungkunakorn, Pojjanaporn Krichtitayawuth, Pallapa Wongsetthachai, Sirintorn Yibchok-Anun, Janenuj Wongtavatchai Jun 2004

The Quantitative Analysis Of Oxytetracycline In Black Tiger Shrimps (Penaeus Monodon) Muscle Tissue And Hepatopancreas, After Oral Medication, Mathu Bumrungkunakorn, Pojjanaporn Krichtitayawuth, Pallapa Wongsetthachai, Sirintorn Yibchok-Anun, Janenuj Wongtavatchai

The Thai Journal of Veterinary Medicine

270, 3-month old, Black Tiger Shrimps (Penaeus monodon) (average 24.55 grams b.w.) were fed for 5 days with oxytetracycline (OTC) medicated feed (coated with squid oil) at 2000 milligrams of OTC per kilogram of feed (2000 ppm). OTC in the hepatopancreas and muscle was measured using High Performance Liquid Chromatography (HPLC) (96 percentage recovery). Samples were taken 0.5, 1, 4, 12 and 24 hours after first administration and 1, 5, 7, 10,14, 21 days after the 5-day treatment. The OTC concentration in samples from fifty negative control shrimps was undetectable by this method. The maximum concentration of OTC (Cmax) detected …


Tape Worm Joyeuxiella Sp. Infection In A Cat: A Case Report, Sudchit Chungpivat, Anudep Rungsipipat, Komkrich Tienkum Mar 2004

Tape Worm Joyeuxiella Sp. Infection In A Cat: A Case Report, Sudchit Chungpivat, Anudep Rungsipipat, Komkrich Tienkum

The Thai Journal of Veterinary Medicine

A four years old, mixed breed cat was reported to have a Joyeuxiella sp. tapeworm infection. Upon necropsy, The most striking lesion was a diffuse chronic catarrhal enteritis along with a heavy tapeworm infection in the small intestine. The tapeworms were identified as the genus Joyeuxeilla, Family Dilepididae, based on the morphology which were comprised of 2 sets of reproductive organs in a mature segment and only one egg in each egg capsule, in the gravid segments. This is the first report of Joyeuxiella sp. tapeworm infection in Thailand.


Effects Of Beta And Kappa Casein On Breeding Values Predictions In Dairy Cattle, Chortip Aroondechachai, Chancharat Reodecha, Monchai Duangjinda, Duangsmorn Suwattana Mar 2004

Effects Of Beta And Kappa Casein On Breeding Values Predictions In Dairy Cattle, Chortip Aroondechachai, Chancharat Reodecha, Monchai Duangjinda, Duangsmorn Suwattana

The Thai Journal of Veterinary Medicine

The objective of this study was to estimate the genetic parameters and breeding values for milk yield, protein yield and protein percentage in 87 crossbred dairy cattle with pedigree and production records and beta and kappa casein genotypes. The data was analyzed using three mixed model equations. The first model included fixed effects and random effects, identifying additive and permanent environments. The second model was similar to the first model using beta (a) or kappa (b) casein genotype (single gene) effect. The third model was the same as the first model but used composite beta and kappa casein genotype (multigene) …


Antigen Distribution Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) In Thai Crossbred Pigs Using Immunohistochemistry, Porntip Laohasittikul, Nuttapon Boonarpha, Yongyut Pongprapachuen, Sawang Kesdangsakonwut, Supradit Wangnaitham, Roongroje Thanawongnuwech Mar 2004

Antigen Distribution Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) In Thai Crossbred Pigs Using Immunohistochemistry, Porntip Laohasittikul, Nuttapon Boonarpha, Yongyut Pongprapachuen, Sawang Kesdangsakonwut, Supradit Wangnaitham, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

Twenty, 3-week-old, crossbred pigs from a herd free from porcine reproductive and respiratory syndrome virus (PRRSV) were inoculated intranasally and intramusculary (1 ml each 102.5 TCID50/ml) with Thai PRRSV isolates of the US genotype (01NP1) (n=9) or the EU genotype (02SB3) (n=8). Three pigs were used as negative controls. Three infected and one control group pig were euthanatized 5, 9 or 15 days postinoculation (DPI) to detect and to compare the location and the amount of PRRSV-antigen present. Immunohistochemical evaluation of formalin-fixed tissues revealed PRRSV-antigen in the pulmonary macrophages of the lungs (6/9, 8/8 and 0/3 of the 01NP1-infected, 02SB3-infected …


Evaluation Of The Aflatoxin B1 Adsorption Capacity Of Bentonite Using An In Vitro Method Mimicking Monogastric Gastro-Intestinal Tract Conditions, Piyarat Chansiripornchai, Johanna Fink-Gremmels Mar 2004

Evaluation Of The Aflatoxin B1 Adsorption Capacity Of Bentonite Using An In Vitro Method Mimicking Monogastric Gastro-Intestinal Tract Conditions, Piyarat Chansiripornchai, Johanna Fink-Gremmels

The Thai Journal of Veterinary Medicine

Aflatoxins are considered as unavoidable contaminants of food and animal feeds, since their production cannot be prevented successfully using current agricultural practices. The use of aflatoxin adsorbents as feed supplements is one of the most promising approaches to reduce aflatoxicoses in animals. Since there are numerous adsorbents marketed, an effective and practical method for the rapid evaluation of adsorption capacity of individual products is needed. In this study, we used an in vitro model designed to mimic the temperature, pH and the time of passage through the stomach and the intestinal tract of a monogastric animal, for the evaluation of …


Peracute Infection Of Actinobacillus Pleuropneumoniae Serotype 1 In Guinea Pigs, Anongnart Somchit, Pornchalit Assavacheep, Wantanee Neramitmansook, Achariya Sailasuta Mar 2004

Peracute Infection Of Actinobacillus Pleuropneumoniae Serotype 1 In Guinea Pigs, Anongnart Somchit, Pornchalit Assavacheep, Wantanee Neramitmansook, Achariya Sailasuta

The Thai Journal of Veterinary Medicine

This experiment was performed to study gross and microscopic lung lesions after endotracheal inoculation with Actinobacillus pleuropneumoniae (A. pleuropneumoniae) serotype 1 in twenty-four guinea pigs. Four-six hours post inoculation, clinical signs of respiratory distress and epistasis were observed. Most of the infected animals suddenly died during this time. The differences between the pulmonary lesions were evaluated. In this experiment, the lesions of peracute infection by A. pleuropneumoniae were induced within 4-6 hours and the lesions of subacute infection, characterized by fibrinopurulent pleuritis were observed in surviving animals 18-20 hours post inoculation. The results suggested that guinea pigs could be used …