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Full-Text Articles in Medicine and Health Sciences
Ang Ii At(1) And At(2) Receptors In Developing Kidney Of Normal Microswine, Susan P. Bagby, Linda S. Lebard, Zaiming Luo, Bryan E. Ogden, Christopher Corless, Elizabeth D. Mcpherson, Robert C. Speth
Ang Ii At(1) And At(2) Receptors In Developing Kidney Of Normal Microswine, Susan P. Bagby, Linda S. Lebard, Zaiming Luo, Bryan E. Ogden, Christopher Corless, Elizabeth D. Mcpherson, Robert C. Speth
HPD Articles
To identify an appropriate model of human renin-angiotensin system (RAS) involvement in fetal origins of adult disease, we quantitated renal ANG II AT(1) and AT(2) receptors (AT1R and AT2R, respectively) in fetal (90-day gestation, n = 14), neonatal (3-wk, n = 5), and adult (6-mo, n = 8) microswine by autoradiography ((125)I-labeled [Sar(1)Ile(8)]ANG II+cold CGP-42112 for AT1R, (125)I-CGP-42112 for AT2R) and by whole kidney radioligand binding. The developmental pattern of renal AT1R in microswine, like many species, exhibited a 10-fold increase postnatally (P < 0.001), with maximal postnatal density in glomeruli and lower density AT1R in extraglomerular cortical and outer medullary sites. With aging, postnatal AT1R glomerular profiles increased in size (P < 0.001) and fractional area occupied (P < 0.04), with no change in the number per unit area. Cortical levels of AT2R by autoradiography fell with age from congruent with 5,000 fmol/g in fetal kidneys to congruent with 60 and 20% of fetal levels in neonatal and adult cortex, respectively (P < 0.0001). The pattern of AT2R binding in postnatal pig kidney mimicked that described in human and simian, but not rodent, species: dense AT2R confined to discrete cortical structures, including pre- and juxtaglomerular, but not intraglomerular, vasculature. Our results provide a quantitative assessment of ANG II receptors in developing pig kidney and document the concordance of pigs and primates in developmental regulation of renal AT1R and AT2R.
Health Professions Division Catalog Academic Year 2002-2003, Nova Southeastern University
Health Professions Division Catalog Academic Year 2002-2003, Nova Southeastern University
Health Professions Divisions Course Catalogs and Course Descriptions
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