Medicine and Health Sciences Commons^™

Further Characterization Of A Complement-Sensitive Mutant Of A Virulent Avian Escherichia Coli Isolate, Theodore J. Kottom, Lisa K. Nolan, Michael Robinson, John Brown, Tom Gustad, Shelley M. Horne, Catherine W. Giddings

Lisa K. Nolan

An attempt was made to characterize the mechanism of complement resistance operating in a virulent avian Escherichia coli isolate. Using flow cytometry to detect antibody to C3, we found that there was significantly more antibody bound to a complement-sensitive mutant of this wild type than to the parent organism, suggesting that more C3 subunits were bound to the wild type. Neither the wild type nor the mutant degraded C3. Further, the mutant was phagocytosed to a significantly greater degree than the wild type by cultured phagocytes in the presence of C5-deficient serum. These data suggest that the wild type is …

A Highly Adherent Phenotype Associated With Virulent Bvg+-Phase Swine Isolates Of Bordetella Bronchiseptica Grown Under Modulating Conditions, Karen B. Register, Mark R. Ackermann

Mark R. Ackermann

The ability of Bvg(-)-phase and Bvg(+)-phase Bordetella bronchiseptica swine isolates, grown under modulating or nonmodulating conditions, to adhere to swine ciliated nasal epithelial cells was determined. When virulent strains were cultivated at 37 degrees C in the Bvg+ phase, numerous adherent bacteria (approximately eight per cell, depending on the strain used) were observed. However, when such strains were grown under modulating conditions (23 degrees C), a significant increase in the level of attachment was seen, suggesting that B. bronchiseptica produces a Bvg-repressed adhesin under these conditions. bvg mutant strains, including an isogenic bvgS mutant, adhered minimally. Western blots indicated that …

Signal Transduction: Rhomantic Interludes Raise Blood Pressure, Andrew P. Somlyo

Jama S. Coartney

No abstract provided.

Sequential Expression Of Egr-1 And Egr-3 In Hippocampal Granule Cells Following Electroconvulsive Stimulation, Kevin O'Donovan

Kevin O'Donovan

No abstract provided.

Spiroplasma Lineolae Sp. Nov., From The Horsefly Tabanus Lineola (Diptera : Tabanidae), Frank E. French, Robert F. Whitcomb, Joseph G. Tully, Patricia Carle, Joseph M. Bové, Roberta B. Henegar, Jean R. Adams, Gail E. Gasparich, David L. Williamson

Gail Gasparich

Collaborative Roles For C-Jun N-Terminal Kinase, C-Jun, Serum Response Factor, And Sp1 In Calcium-Regulated Myocardial Gene Expression, Patrick M. Mcdonough, Deanna S. Hanford, Amy B. Sprenkle, Noel R. Mellon, Christopher C. Glembotski

Amy Sprenkle

Entrapped Lip Following Sport Injury, W.C. Ngeow, L. Kon

Wei Cheong Ngeow

A case is presented of a patient whose lip became entrapped onto his orthodontic bracket following a blow to his face during a contact sport. The entrapped mucosa had to be released surgically.

Effects Of Pasteurella Multocida Toxin On Porcine Bone Marrow Cell Differentiation Into Osteoclasts And Osteoblasts, S. M. Gwaltney, R. J. S. Galvin, K. B. Register, R. B. Rimler, Mark R. Ackermann

Mark R. Ackermann

The effect of Pasteurella multocida toxin (PMT) on porcine osteoclast and osteoblast differentiation was studied using in vitro cell culture systems. When grown in the presence of Vitamin D3, isolated porcine bone marrow cells formed multinucleated cells with features characteristic of osteoclasts. Exposure of bone marrow cells to Vitamin D3 and PMT during growth resulted in formation of increased numbers and earlier appearance of osteoclasts compared to controls. Ultrafiltered medium from PMT-treated cells likewise increased osteoclast numbers, suggesting that a soluble mediator may be involved in the action of PMT. When cell cultures were treated with fluorescein-labeled PMT, fluorescence was …

Iron Regulatory Protein 1 Is Not Required For The Modulation Of Ferritin And Transferrin Receptor Expression By Iron In A Murine Pro-B Lymphocyte Cell Line, Kevin Schalinske, Kenneth P. Blemings, Daniel W. Steffen, Opal S. Chen, Richard S. Eisenstein

Kevin Schalinske

Iron regulatory proteins (IRPs) are cytoplasmic RNA binding proteins that are central components of a sensory and regulatory network that modulates vertebrate iron homeostasis. IRPs regulate iron metabolism by binding to iron responsive element(s) (IREs) in the 5* or 3* untranslated region of ferritin or transferrin receptor (TfR) mRNAs. Two IRPs, IRP1 and IRP2, have been identified previously. IRP1 exhibits two mutually exclusive functions as an RNA binding protein or as the cytosolic isoform of aconitase. We demonstrate that the BayF3 family of murine pro-B lymphocytes represents the first example of a mammalian cell line that fails to express IRP1 …

A Conceptual Model Of The Corporate Decision-Making Process Of Sport Sponsorship Acquisition, David Arthur, Don Scott, Terry Woods

Terry Woods

The general acceptance of sport sponsorship by corporate and sporting worlds alike has led to a situation whereby corporations receive from hundreds to thousands of unsolicited proposals annually. Despite this, there is a general lack of systematic research into sport sponsorship with little information in existence as to how corporations decide between alternative properties. In an attempt to address this situation, this paper develops a conceptual model of the sport sponsorship acquisition process based on the basic tenets of organizational purchasing behavior, contemporary literature on sport sponsorship, and the results of a qualitative study. It is anticipated that the Sport …

The Use Of Gutta-Percha Point To Locate The Origin Of Facial Sinus, W.C. Ngeow

Wei Cheong Ngeow

Infection from the wisdom teeth usually causes severe swelling at the region of the angle and body of the mandible. Occasionally, it tracts outward to form a cervicofacial sinus. This paper demonstrates the use of gutta-percha point to locate the origin of a cervicofacial sinus due to an asymptomatic impacted wisdom tooth. The advantage of using gutta-percha point is discussed.

Effect Size And Power For Clinical Trials Using Years Of Healthy Life As The Primary Endpoint, Paula Diehr

Paula Diehr

Some clinical trials perform repeated measurements on patients over time, plot those measures against time, and summarize the results in terms of the area under the curve. If the measured variable is health status, the summary outcome is sometimes referred to as years of healthy life (YHL), or quality-adjusted life years (QALY). This paper investigates some theoretical and practical aspects of randomized trials designed to assess measures such as YHL. We first derived algebraic expressions for the effect size of YHL measures under several theoretical models of the treatment's effect on health. We used these expressions to examine how the …

Effect Of Thermocycling On The Fracture Toughness Of Composites, Noor Hayaty Abu Kasim Abu Kasim N.H.

Noor Hayaty Abu Kasim Abu Kasim N.H.

Fracture toughness has been recognised to be one of the most important mechanical property for retorative materials. It is the aim of this study to investigate the effect of thermocycling an fracture toughness of a range of dental composites; P50(P)1 , Silux Plus(S)2, Heliomolar(H)3, Clearfil Photo Posterior-light activated(CLA)4 and Clearfil Posterior-chemically activated(CC)3. 110 rectangular pre-notched specimens was prepared for each material utilising a stainless steel mould. The specimens were divided into 11 groups of 10 each. Test groups 1-6 were subjected to 0, 250, 500, 750, 1000 and 10,000 thermal cycles respectively. All specimens were subjected to a 3 point …

Hacks, Flacks And Counter-Attacks: Cigarette Advertising, Sponsored Research And Controversies, Richard W. Pollay

Richard W. Pollay

No abstract provided.

Attitudes And Perceptions Of Nurses Regarding Early Communication Intervention, Brenda Louw, Ingrid Weber

Brenda Louw

Decreased Intracellular Survival Of An Fkpa Mutant Of Salmonella Typhimurium Copenhagen, Shelley M. Horne, Theodore J. Kottom, Lisa K. Nolan, Kevin D. Young

Lisa K. Nolan

The fkpA gene of Salmonella typhimurium encodes a protein similar to the macrophage infectivity potentiator (Mip) proteins of Legionella pneumophila and Chlamydia trachomatis. Because Mip proteins enhance the ability of these intracellular pathogens to survive within macrophages and epithelial cells, we tested whether the product of the fkpA gene would have the same effect on the intracellular growth of a virulent strain of S. typhimurium. By a series of P22 transductions, the fkpA gene of S. typhimurium Copenhagen was replaced with the inactive fkpA1::omega-Cm gene from Escherichia coli, creating the mutant S. typhimurium KY32H1. The Copenhagen and KY32H1 strains were …

Isolation Of A Mannose-Binding And Ige- And Igm-Reactive Lectin From The Seeds Of Artocarpus Integer, Onn Haji Hashim

Onn Haji Hashim

A mannose-binding lectin, termed champedak lectin-M, was isolated from an extract of the crude seeds of champedak (Artocarpus integer). On gel filtration chromatography, the lectin eluted in a single peak at elution volumes corresponding to 64 kDa, SDS-PAGE showed the mannose-binding lectin to be composed of 16.8 kDa polypeptides with some of the polypeptides being disulphide-linked to give dimers. When tested with all isotypes of immunoglobulins, champedak lectin-M demonstrated a selective strong interaction with human IgE and IgM, and a weak interaction with IgA2, The binding interactions of lectin-M were metal ion independent. The lectin was also shown to interact …

Health Enhancing Physical Activity (Hepa) In Greece, Yanni D. Afthinos

Yanni D Afthinos

No abstract provided.

Microleakage Testing, Noor Hayaty Abu Kasim Abu Kasim N.H.

Noor Hayaty Abu Kasim Abu Kasim N.H.

Microleakage testing has been used to determine the possible clinical performance of a restorative material. Many microleakage testing materials have been developed and performed through the years. There has been no agreement as to which testing methodology would give the most accurate results. Attempts have been made to simulate the oral conditions and to give a more quantitative representation of micro leakage. The different micro leakage testing methodologies are presented in this paper.

A Rolling-Ball Device For Producing Surface Fatigue And Its Application To Dental Materials, Noor Hayaty Abu Kasim Abu Kasim N.H.

Noor Hayaty Abu Kasim Abu Kasim N.H.

A new method of producing and evaluating surface fatigue using a rolling-ball device has been developed. The method involves constraining a rolling ruby ball between the ''v'' groove of a rotor and the test specimen. The ball applies a compressive stress to the surface of the test material whilst it rolls in a circular pattern across the specimen surface. The fatigue life is defined as the time taken for surface degradation to begin to occur. The method is simple and reproducible and allows fatigue data to be gathered using a relatively small number of specimens. A series of model dental …

Neuroendocrine Aspects Of Primary Endogenous Depression Xv. Mathematical Modeling Of Nocturnal Melatonin Secretion In Major Depressives And Normal Controls, L. Kathleen Sekula, Joseph F. Lucke, E. Kevin Heist, R. Kenneth Czambel, Robert T. Rubin

Joseph Lucke

We previously reported a trend toward a higher mean nocturnal serum melatonin (MEL) concentration, based on 30-min blood sampling over 24 h, in 23 female definite endogenous depressives compared to 23 matched normal female control subjects, and no significant difference in 15 male depressives compared to their controls (Rubin et al., 1992). In both groups of patients vs. their controls, there also were trends toward an earlier MEL rise time, by about 30 min, and a later MEL peak time, by about 90 min. Because the offset of MEL secretion was not estimated in that study, the total duration of …

A Porcine Model For The Evaluation Of Virulence Of Bordetella Bronchiseptica, Mark R. Ackermann, K. B. Register, C. Gentry-Weeks, S. M. Gwaltney, T. Magyar

Mark R. Ackermann

Studies of virulence factors of Bordetella bronchiseptica require a suitable system. Such a system was devised in colostrum-deprived, caesarean-derived pigs, aged 7 d. In two different experiments, pigs (n=11) were inoculated intranasally with 106 colony-forming units of the virulent strain 4609. In the same way, further pigs (n=11) were inoculated with a strain (B133) of unknown virulence. No significant differences between 4609 and B133 colonization were seen. However, colonization of the turbinates was significantly higher than that of the trachea, lung and tonsil, and a significantly higher degree of colonization was present at 11 d post-inoculation (PI) than at 15 …

Sexual Orientation: A Cultural Diversity Issue For Nursing, Tr Misener, Rl Sowell, Kenneth D. Phillips, C.M. Harris

Kenneth D. Phillips

Traditional approaches to cultural diversity and the development of a culturally aware workforce have consistently ignored the importance of gender role orientation and sexual orientation as sources of potential conflict in the work environment. To maintain its integrity as a caring profession, nursing must take steps to end personal and professional discrimination on the basis of sexual orientation.

Soil Ingestion: A Concern For Acute Toxicity In Children, Edward J. Calabrese, Edward J. Stanek, Robert C. James, Stephen M. Roberts

Edward J. Stanek

Several soil ingestion studies have indicated that some children ingest substantial amounts of soil on given days. Although the EPA has assumed that 95% of children ingest 200 mg soil/day or less for exposure assessment purposes, some children have been observed to ingest up to 25-60 g soil during a single day. In light of the potential for children to ingest such large amounts of soil, an assessment was made of the possibility for soil pica episodes to result in acute intoxication from contaminant concentrations the EPA regards as representing conservative screening values (i.e., EPA soil screening levels and EPA …

Asthma Mortality In The United States Has Risen But Is Similar To That In England And Wales, David M. Mannino

David M. Mannino

No abstract provided.

A National Epidemiological Survey Of Oral Mucosal Lesions In Malaysia, Rosnah Binti Zain, Ikeda N., Razak I. A., Axéll T., Majid Z. A., Gupta P. C., Yaacob M.

Prof. Dr. Rosnah Binti Zain

The prevalence of oral mucosal lesions in Malaysia was determined by examining a representative sample of 11707 subjects aged 25 years and above throughout the 14 states over a period of 5 months during 1993/1994. A two-stage stratified random sampling was undertaken. A predetermined number of enumeration blocks, the smallest population unit in the census publication, was selected from each state. With the selected enumeration block, a systematic sample of living quarters was chosen with a random start. The survey instrument included a questionnaire on sociodemographic characteristics and a clinical examination. The clinical examination was carried out by 16 specially …

The Effect Of Silica Inhibition Of Macrophage On Renal Allograft Rejection, Carman A. Giacomantonio

Vivian C. McAlister

Spinal Cord Injury - Special Interest Group Combined Sections Meeting: Thursday, Feb. 13, 1997 Business Meeting Minutes, Sarah A. Morrison

Sarah Morrison, PT, MBA, MHA

No abstract provided.

The Role Of Rejection In Aortic Valve Allograft Failure, Ahmad Moustapha

Vivian C. McAlister

Aortic Valve Grafts In The Rat: Evidence For Rejection., Ahmad Moustapha, David Ross, B Bittira, D Vanvelzen, C L. Lannon, Vivian C. Mcalister, Tim D. Lee

Vivian C. McAlister