Medicine and Health Sciences Commons^™

Studies Of Plasma Membrane Domain-Specific Molecules In Isolated And Cultured Rat Hepatocytes, Pamela Leslie Zeitlin

Yale Medicine Thesis Digital Library

Single rat hepatocytes, prepared by collagenase perfusion of liver, lost the three morphologically and functionally distinct plasma membrane domains that exist in situ (sinusoidal, bile canalicular, and lateral). Hepatocytes placed in monolayer culture re-established morphologically distinct plasma membrane regions analogous to the original three domains. The behavior of several domain-specific markers was studied in these cells. The asialoglycoprotein (ASGP) receptor, an integral membrane glycoprotein normally restricted at the surface to the sinusoidal domain in situ, was retained in both single hepatocytes (> 95%) and in cells after 24 hr in monolayer culture but at lower levels. Cytochemistry with asialo-oroso-mucoid-horseradish peroxidase …