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Full-Text Articles in Medicine and Health Sciences
Rna-Dependent Selenocysteine Biosynthesis In Eukaryotes And Archaea, Sotiria Palioura
Rna-Dependent Selenocysteine Biosynthesis In Eukaryotes And Archaea, Sotiria Palioura
Yale Medicine Thesis Digital Library
Selenocysteine (Sec), the 21st genetically encoded amino acid, is the major metabolite of the micronutrient selenium. Sec is inserted into nascent proteins in response to a UGA codon. The substrate for ribosomal protein synthesis is selenocysteinyl-tRNASec. While the formation of Sec-tRNASec from seryl-tRNASec by a single bacterial enzyme selenocysteine synthase (SelA) has been well described, the mechanism of Sec-tRNASec formation in archaea and eukaryotes remained poorly understood. Herein, biochemical and genetic data provide evidence that, in contrast to bacteria, eukaryotes and archaea utilize a different route to Sec-tRNASec that requires the tRNASec-dependent conversion of O-phosphoserine (Sep) to Sec. In this …
Nmr Studies Of Acyl Chain-Protein Interactions, Mark Christian Oswood
Nmr Studies Of Acyl Chain-Protein Interactions, Mark Christian Oswood
Yale Medicine Thesis Digital Library
An approach employing heteronuclear NMR is presented for monitoring the interactions between long-chain fatty acids and proteins which are involved in their synthesis and intracellular transport. Two naturally occurring fatty acids, hexadecanoic (palmitic) and cis-9-10-methylene-hexadecanoic (MHA) acids were labeled using a biosynthetic scheme with 13C acetate as a source. The biosynthetic incorporation of 13C from a two carbon source resulted in sequential pairs of 13C atoms in the product. When combined with double quantum spectroscopy, a highly efficient suppression of natural abundance background resonances results. Proton-detected two and three-dimensional experiments which correlate double quantum carbon chemical shifts with proton shifts …
Basement Membrane Polarizes Epithelial Cells And Its Loss Can Result In Neoplastic Disorganization, Donald Elliot Ingber
Basement Membrane Polarizes Epithelial Cells And Its Loss Can Result In Neoplastic Disorganization, Donald Elliot Ingber
Yale Medicine Thesis Digital Library
The studies described in this dissertation demonstrate that basement membrane may be required for maintenance of organized epithelial tissue architecture. The structural organization of normal rat pancreatic acinar epithelium is fully characterized in order to analyze its neoplastic disorganization within a pancreatic acinar cell tumor. Transmission electron microscopy and indirect immunofluorescence microscopy of frozen semithin tissue sections are utilized to localize different components of the plasmalemma, cytoskeleton, basement membrane and connective tissue. Normal acinar cells sit on a continuous basement membrane and display a polarized distribution of intracellular organelles, cytoskeletal elements, and distinct membrane domains while these organized cell relations …
Neutron Diffraction Analysis Of The Structure Of Rod Photoreceptor Membranes In Intact Retinas, Mark Jay Yeager
Neutron Diffraction Analysis Of The Structure Of Rod Photoreceptor Membranes In Intact Retinas, Mark Jay Yeager
Yale Medicine Thesis Digital Library
No abstract provided.
Limited Fragmentation Of Specifically Labeled Rhodopsins, Jordan S. Pober
Limited Fragmentation Of Specifically Labeled Rhodopsins, Jordan S. Pober
Yale Medicine Thesis Digital Library
No abstract provided.