Medicine and Health Sciences Commons^™

Green Engineering And Gate-To Gate Life Cycle Assessments For Pharmaceutical Products, Daniel Fichana

Theses and Dissertations

The research of this thesis focused on the environmental and processing metrics during the development of two different drugs. Previous research in life cycle assessments and green engineering have focused on other products and processes, but only a limited amount of studies have been conducted for pharmaceutical applications. This analysis concerned a gate-to-gate analysis of two distinct pharmaceutical products along with the development of a solvent selection table. The goal of this research was to determine how various processing and environmental metrics were affected by process improvements.

The first drug was the pravastatin, which was made via a fermentation route. …

Psychometrically Equivalent Digital Recordings For Speech Audiometry Testing In Mandarin Chinese: Standard Mandarin Dialect, Lara-Jill Jennings

Theses and Dissertations

The development of digitally recorded speech audiometry materials in Mandarin Chinese has been limited to date. High quality materials proliferate in the English language and have been developed for other languages such as Brazilian Portuguese, Spanish, Japanese, Korean, French, Italian, Polish, and Russian. The aims of this study were to develop, digitally record, evaluate, and equate words and word lists in Mandarin Chinese to use for speech reception threshold testing and for speech discrimination testing. The words that were evaluated were chosen from a Chinese frequency usage dictionary. One native male and one native female talker recorded the words onto …

Event Related Potentials: A Study Of The Processing Of Gapping Structures In Adolescents, Michelle Miller Nishida

Theses and Dissertations

Many questions remain unanswered regarding the intricacies of the human brain, especially with regard to the complexities of language processing. One essential component of human sentence processing is the ability to detect, decipher, and recover from errors in the interpretation of both verbal and written language. This process of repair of ungrammatical sentences and revision or reinterpretation of ambiguous sentences has been studied extensively in recent years. A variety of tools have been developed, including the use of event-related potentials (ERPs) in order to assess how language is processed and developed, and to help better identify the nature of these …

Effectiveness Of Story Enactments Versus Art Projects In Facilitating Preschool Children's Story Comprehension, Jennifer Ann Johnson

Theses and Dissertations

The purpose of this study was twofold: (a) to compare preschool children's comprehension of a story after either enacting the story or participating in an art project, and (b) to qualitatively describe the children's interactions during the more interactive story enactment instruction. Twenty children from two Head Start classrooms were told the stories as a class, and then participated in either an art project (AP) or story enactment (SE) in small groups. The children in each classroom each heard three stories followed by the AP condition, and three followed by the SE condition. The children's comprehension of the story was …

The Effect Of A Lingual Magnet On Fricative Production: An Acoustic Evaluation Of Placement And Adaptation, Andrea Lynn Weaver

Theses and Dissertations

Much of speech kinematics research is conducted by attaching a device to the articulators. However very little research has been conducted to determine what influence these devices may have on the perceptual and acoustic characteristics of speech. This study examined the effect of placing a small magnet on the tongue of ten normal adult speakers while reading a sentence containing /s/ and "sh" in initial, medial and final position. Two different placements of 10 and 15 mm from the tip of the tongue were analyzed. Data were taken before magnet placement, immediately after magnet placement, after 5 minutes of conversation, …

How Individuals With Parkinson's Disease Modify Their Speech In A Repetition For Clarification, Lynn Marie Watkins

Theses and Dissertations

The speech of individuals with Parkinson's disease (PD) is typically characterized as lacking in proper prosody because of its monopitch and monoloud quality, in addition to its reduced intensity. These qualities make it difficult for others to understand speakers with PD. The purpose of the current study was to identify what individuals with PD would do vocally, if anything at all, to improve speech production following a simulated misunderstanding of what they had just said. The study evaluated the performance of 5 individuals with PD and compared their performance to 5 age- and sex-matched controls. Specifically, measures of vocal intensity …

Exploration Of Lip Shape Measures And Their Association With Tongue Contact Patterns, Jessica Lynn Wagner

Theses and Dissertations

A variety of tools and techniques have been developed to measure the movements of the vocal tract, specifically of the tongue and lips. In recent years, computer technology has allowed for extensive exploration of these precise movements and for the development of speech recognition systems. However, there has been relatively little work on the combination of visible facial movements and internal articulatory activity. In this study, two different technologies were used to explore the internal and external movements of speech production in eight speakers: palatometry quantified tongue contact patterns and computerized video image analysis was used to derive lip shape …

Predicting Body Mass Index In Northern Plains American Indian Children, Tami Jollie-Trottier

Theses and Dissertations

Obesity has become a major health concern for American Indians. Obesity prevalence is higher for minority groups, however American Indians consistently have higher rates than any other U.S. population. Of more concern is the trend towards higher rates of overweight and obesity in American Indian children. Obesity has been associated with many health concerns such as coronary heart disease, high blood pressure, and type 2 diabetes mellitus in both American Indian adults and children. The purpose of this study is to identify predictor variables that may be contributing to the development or possibly maintenance of obesity in American Indian children. …

The Effects Of Divided Attention On Speech Motor, Verbal Fluency And Manual Motor Task Performance, Erin Hamblin

Theses and Dissertations

Research in dual task performance varies widely in its methodology and results. The present study employed three different types of activity to provide insights into the interference that occurs in dual task performance. Twenty young adults completed a speech task (repeating a sentence), a verbal fluency task (listing words beginning with the same letter), and right- and left-handed motor tasks (placing pegs and washers in a peg board) in isolation and in concurrent conditions. Speech kinematic data revealed that during concurrent performance of manual tasks, lip displacement and peak velocity decreased, while sound pressure level and spatiotemporal variability increased. The …

Auditory And Visual Correlates Of The Processing Of Gapping Structures In Adults, Tara Hansen

Theses and Dissertations

The purpose of this study was to compare event-related potential (ERP) effects of speech processing and effects in sentence reading elicited by sentences containing gapping structures, or a "missing" verb. N400 and P600 waveforms were collected in 20 adults between 18 and 30 years of age. Two experiments were conducted with each participant. In the two experiments ERP recordings were collected as sentences, some containing gapping structures, were presented to the subjects. In one experiment sentences were presented through headphones in sentences spoken at normal rate and with normal intonation. In the second experiment sentences with the same gapping structures …

Performance Intensity Functions For Digitally Recorded Japanese Speech Audiometry Materials, Tanya Crawford Mangum

Theses and Dissertations

The purpose of this study was to develop digitally recorded speech audiometry materials in the Japanese language to evaluate Speech Reception Threshold (SRT) and speech discrimination. Trisyllabic words were used to evaluate the SRT and bisyllabic words were used for speech discrimination. Words were recorded by one native female talker and one native male talker who were judged as having standard Japanese dialects. Twenty native Japanese speakers between the ages of 20 and 32 were used as subjects to evaluate 69 trisyllabic words across 13 different intensity levels. The 25 trisyllabic words with the steepest psychometric function (%/dB) were selected …

An Investigation Of Vocal Abuse In School Teachers, Jodi L. Franczak

Theses and Dissertations

The purpose of this study was to examine the effects of vocal abuse in school teachers and teacher's aides pre and post one academic term. Vocal abuse was defined as any of several behaviors, including long periods of talking, yelling, smoking, consuming alcohol, etc. that can result in damage to the laryngeal mechanism. Initially, the participants of the study included fifteen elementary school teachers and five elementary teacher's aides from two schools: the Red Lake Madsen Public School, and Golden Learning Center. Six participants were excluded from the study for various reasons. Participants were randomly assigned to either Group 1 …

An Analysis Of Biometric Technology As An Enabler To Information Assurance, Darren A. Deschaine

Theses and Dissertations

The use of and dependence on, Information technology (IT) has grown tremendously in the last two decades. Still, some believe the United States is only in the infancy of this growth. This explosive growth has opened the door to capabilities that were only dreamed of in the past. As easy as it is to see how advantageous this technology is, it also is clear that with its advantages come distinct responsibilities and new problems that must be addressed. For instance, the minute one begins using information processing systems, the world of information assurance (IA) becomes far more complex. As a …

Assessing The Relationship Between Socioeconomic Variables And Risk Of Overweight Among Children 6 Years Of Age, Marilyn Cochon Batan

Theses and Dissertations

Background: The percentage of overweight children has tripled in the last thirty years. Inconsistent findings are published regarding the relationship between socioeconomic variables and being overweight in childhood.Objective: To determine whether socioeconomic variables are associated with risk of being overweight among six year olds. Methods: Six year olds with BMI data were selected from the National Survey of Children's Health (n=4,362). Variables were coded and a low socioeconomic index was created. Using population weights, descriptive statistics were generated and regression was utilized to assess the relationship between socioeconomic variables and being 'at risk' for overweight. Socioeconomic variables were also compared …

Characterization Of The Zona Incerta, Heather Joyce Green

Theses and Dissertations

Parkinson's Disease affects more than 1 million people in the United States with 60,000 new cases being diagnosed each year. Currently, there is no cure for Parkinson's Disease, but there are several treatment options available. Currently the most popular surgical option is Deep Brain Stimulation. Microelectrode recording helps identify nuclei as the microelectrode passes through them. While the firing frequencies of the target nuclei are well defined, other nuclei are not. This study will attempt to characterize the Zona Incerta, which is the structure directly above the Subthalamic Nucleus, a target nucleus. Characterization of the firing frequency of the Zona …

The Study Of The Effect Of Drugs Of Abuse On Protein Kinase A Activity In Mouse Brain And Spinal Cord, George D. Dalton

Theses and Dissertations

Morphine and Δ9-THC are drugs that produce analgesia and rewarding effects. However, chronic treatment with morphine and Δ9-THC produces problematic side-effects including tolerance and physical dependence. The cellular mechanisms underlying opioid and cannabinoid antinociceptive tolerance have been studied for years. Research has demonstrated that the expression of morphine and Δ9-THC antinociceptive tolerance may be mediated through intracellular signaling pathways, such as the adenylyl cyclase /Protein Kinase A (PKA) cascade. The present study investigated the role of PKA in the expression of morphine and Δ9-THC antinociceptive tolerance. Male Swiss Webster mice were treated chronically with morphine or Δ9-THC and the warm-water …

Predictors Of Induced Abortion Among Female Youth Center Users In Port-Au-Prince, Haiti, Rachel Barker

Theses and Dissertations

Objective: Induced abortions occur in every country in the world. However sixty percent of the world's women live in countries where abortion is illegal. 19 million pregnancies end in unsafe and/or illegal abortions. Annually, 68,000 women die from unsafe abortions. Legal or not, unsafe abortion is one of the great neglected problems of health care in developing countries like Haiti and is a serious concern to women during their reproductive lives. Methods: The data for this study was collected from youth centers (ages 15-24) and used to estimate percentage distributions of abortion ratios by selected characteristics of women, particularly age …

A Quantitative Comparison Of The Fill Density Of Mta Produced By Two Different Placement Techniques., Priscilla Yeung

Theses and Dissertations

The purpose of this study was to quantitatively compare the fill density of MTA produced by hand condensation and hand condensation with indirect ultrasonic activation. Thirty acrylic block with 30 degree curved canals (group C) and 30 with straight canals (group S) were instrumented to a final apical size of 45 of 0.06 taper crown-down technique. After irrigating with water and drying with paper points, each block was weighed to the nearest 0.0001g with a digital electronic balance. In half of the specimens, chosen at random, the canal first filled with MTA using the hand condensation method (H) then weighed. …

Method Of Infant Feeding As A Predictor Of Maternal Responsiveness, Emily Eiwen Drake

Theses and Dissertations

Infants need to develop effective, secure attachment to their primary caregivers in the first year of life. Researchers have not been able to identify all the factors that may influence the development of infant attachment. Most of the studies in this area have been done without regard to infant feeding as a potential factor. Maternal responsiveness appears to be key in the child's development of secure attachment behaviors, yet even after decades of research on infant attachment and maternal responsiveness, there is little evidence available to assist with early identification of families at risk and few interventions known to be …

Status Epilepticus Results In A Duration-Dependent Increased Protein Kinase A Activity In The Rat Pilocarpine Model, James M. Bracey

Theses and Dissertations

This study was conducted to characterize cellular changes occurring during the progression of status epilepticus (SE) that could lead to the maintenance of increased membrane excitability. SE was induced by injection of pilocarpine after which rats were monitored both electrographically and behaviorally. After various lengths of time in SE, specific brain regions were isolated for biochemical study. SE resulted in an early maintenance of PKA activity in both cortical homogenate and crude synaptoplasmic membrane (crude SPM) fractions. At subsequent stages of SE there was a significant increase in PKA activity in both homogenate and crude SPM fractions. Wester blot analysis …

The Experience Of Decision Making In Adolescent Females And Young Women With Mobility Limiting Disorders, Bernice Balzer Allen

Theses and Dissertations

Young women with mobility limiting disorders face many challenges as they move through adolescence into young adulthood. In addition to the challenges of this developmental period, they must consider their disability as they manage their lives. Competent decision making is a foundational skill for self-management and for transition in adulthood. It is also a protective factor for resilience.There is little research on decision making in adolescents with disabilities. Therefore, the purpose of this qualitative study was to explore the decision making experiences of female adolescents and young women with mobility limiting disorders. Theoretical underpinnings for the study included Resilience Theory …

Variations In Quality Outcomes Among Hospitals In Different Types Of Health Systems, Askar S. Chukmaitov

Theses and Dissertations

Although prior research has found differences in costs and financial performance across different types of hospital systems, there has been no systematic study of variations in patient quality of care or safety indicators across different systems. Our study examines whether five main types of health systems - centralized (CHS), centralized physician/insurance (CPIHS), moderately centralized (MCHS), decentralized (DHS), and independent (IHS) - as well as other hospital characteristics are associated with differences in quality of patient care. Data were assembled for 6 years (1995 - 2000) from multiple sources. We used 4 AHRQ risk adjusted inpatient quality indicators (IQIs) and 5 …

Brucellosis In Alpacas (Lama Pacos) In Communities Of The City Ulla Ulla, Franz Tomayo Province, Department Of La Paz, Macario Suxo Blanco

Theses and Dissertations

This investigation was performed in the Altiplano Altoandino sub-humid region in the Ulla Ulla district, Pelechuco township, Franz Tomayo province, department of La Paz, Bolivia. The objective of this study was to quantify and compare the presence of Brucellosis in male and female alpacas (Lama pacos) at ages 2, 3, 4, 5, 6, and 7 years. Blood samples were taken from the jugular vein of 500 alpacas from the Hichucollo, Huacuchani, Ucha Ucha, and Ulla Ulla communities. The serum from each sample was separated and a serological diagnosis was performed by rapid agglutination with Rose Bengal chemical stain. In the …

Effect Of Medicaid/Schip And Wic On Oral Health Of Low-Income Children, Dahlia Naqib

Theses and Dissertations

Oral caries is the most prevalent chronic disease among US children, and disproportionately impacts those of low socioeconomic status. Studies have shown that the Special Supplemental Nutrition Program for Women Infants and Children (WIC) improves access to dental care among Medicaid children. This study investigated the impact of WIC, Medicaid, and the State Children's Health Insurance Program (SCHIP) on the prevalence of dental caries among low-income children. The 1999-2000 and 2001-2002 NHANES data were utilized for this analysis. Children 2-4 years old who participated in WIC, Medicaid, or SCHIP, or who were uninsured, and for whom both interview and complete …

Identification And Characterization Of The Expression Profile Of Oligodendrocyte-Derived And Associated Proteins Via Unilateral X-Irradation Of The Rat Optic Nerve, Nicholas Greco

Theses and Dissertations

Recent studies examining cell-cell interactions during CNS development and following disease or trauma have highlighted our limited understanding of the in vivo functions of the myelinating cell of the CNS, the oligodendrocyte. With this in mind, our laboratory has developed techniques by which a profile of proteins derived from or regulated by oligodendrocytes can be elucidated. Specifically, we have demonstrated that oligodendrocytes can be selectively eliminated from one optic nerve of a rat by treating the animal with a unilateral exposure of X-irradiation at the time of birth. Consequently, this approach allowed us to experimentally create, within the same animal, …

Long-Term Consequences Of West Nile Virus In Virginia., Diana Cruz Ocampo

Theses and Dissertations

Objective: The purpose of this investigation was to describe the long-term effects and functional outcomes of patients in Virginia who were reported to the Virginia Department of Health with West Nile virus (WNV) non-neuroinvasive and neuroinvasive disease. The study identified the duration of symptoms after initial illness, the number of persons who fully recovered versus the number who continue to be symptomatic and how patients' quality of life differed after illness.Methods: The study population was drawn from 60 human cases that met the surveillance case definition for non-neuroinvasive and neuroinvasive WNV illness in Virginia between 2002-2004. Information was collected during …

Child's Temperament And Conscious Sedation Outcomes, Jennifer Mark Dixon

Theses and Dissertations

The purpose of this study was to investigate the role of child temperament and its effect on the outcome of conscious sedation using the following agents: Chloral Hydrate (35mg/kg), Meperidine (2mg/kg), and Hydroxyzine (2mg/kg).The Emotionality, Activity, Sociability (EAS) Temperament Survey for Children was used to measure the child's temperament. The temperament survey measures three realms (Emotionality, Activity, Sociability/Shyness). The sedation outcomes were rated using the modified North Carolina Behavior Rating Scale (NCBRS) from 1-4 (Quiet, Annoyed, Upset, and Wild). The final sample population consisted of 34 children, 24 girls (71%) and 10 boys (29%), ranging in age from 0-9 years …

Evaluation Of The Pharmacokinetic-Pharmacodynamic Relationship, Metabolism And Plasma Protein Binding Of The Novel Antitumor Agent, 2-Methoxyestradiol (2me2), Following Oral Administration In Patients With Solid Tumors., Nehal Jagdish Lakhani

Theses and Dissertations

The goal of this study was to determine safety, tolerability and pharmacokinetics of 2ME2 in patients with solid tumors and determine maximum tolerated dose (MTD). The following hypotheses were tested: 1) 2ME2 will be well tolerated in clinic when given orally and will have quantifiable effects on the ex vivo markers of angiogenesis and apoptosis; 2) 2ME2 will exhibit linear pharmacokinetics; 3) Plasma protein binding will be extensive and linear; 4) Sulfation will be the major metabolic pathway for 2ME2.This was a phase I dose escalation study. Twenty patients with refractory solid tumors were enrolled. 2ME2 was administered orally starting …

Interleukin-10 Induces Apoptosis In Developing Mast Cells Via A Mitochondrial, Stat3-Dependent Pathway, Daniel Paul Bailey

Theses and Dissertations

Objective. The aim of this study was to determine the effects of interleukin-10 on mast cell development from bone marrow progenitors.Materials and Methods. Unseparated mouse bone marrow cells were cultured in IL-3+SCF, giving rise to mast cells and monocytes/macrophages. The addition of IL-10, and the use of Signal Transducer and Activator of Transcription (STAT)3-deficient bone marrow cells were employed to measure the effects of IL-10 and STAT3 expression on cell viability, proliferation, and differentiation. Bax-deficient and Bcl-2 transgenic bone marrow cells were used to determine the importance of the mitochondria in IL-10-mediated effects.Overview. Mast cells arise from hematopoietic stem cells …

Demographic Variables Affecting Patient Referrals From General Practice Dentists To Periodontists, Mark Roy Zemanovich

Theses and Dissertations

BackgroundWithin dentistry, a limited body of literature exists regarding the referral relationships between general practitioners (GPs) and specialists. The purpose of this study was to investigate the referral relationship between GPs and periodontists within the state of Virginia. MethodsA survey focused on the demographic variables in the referral relationship between GPs and periodontists was developed. The survey was mailed to 800 dentists throughout the state of Virginia. Descriptive statistics was completed along with multivariate logistic regression analysis comparing the responses with the number of patients referred per month to a periodontist. ResultsFemale respondents were more likely to refer three or …