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Full-Text Articles in Medicine and Health Sciences
Novel Automation Of An Enzyme-Linked Immunosorbent Spot Assay Testing Method: Comparable Diagnostic Performance Of The T-Spot.Tb Test Using Manual Density Gradient Cell Isolation Versus Automated Positive Selection With The T-Cell Select Kit, Shu-Hua Wang, Murgesean V. S. Rajaram, Andre Trollip, Qian Wu, Doris Ayala, Danyelle Garza, Marielena A. Benavidez, Kelsie Fox, Genesis P. Aguillón-Durán, Blanca I. Restrepo
Novel Automation Of An Enzyme-Linked Immunosorbent Spot Assay Testing Method: Comparable Diagnostic Performance Of The T-Spot.Tb Test Using Manual Density Gradient Cell Isolation Versus Automated Positive Selection With The T-Cell Select Kit, Shu-Hua Wang, Murgesean V. S. Rajaram, Andre Trollip, Qian Wu, Doris Ayala, Danyelle Garza, Marielena A. Benavidez, Kelsie Fox, Genesis P. Aguillón-Durán, Blanca I. Restrepo
School of Medicine Publications and Presentations
The diagnosis of latent tuberculosis (TB) infection (LTBI) is critical to improve TB treatment and control, and the T-SPOT.TB test is a commercial enzyme-linked immunosorbent spot assay used for this purpose. The objective of the study was to increase automation and extend the time between blood collection and processing for the T-SPOT.TB test from 0 to 8 h to 0 to 54 h. The previous maximum time between blood collection and processing for the T-SPOT.TB test is 32 h using T-Cell Xtend. For this, we compared the T-SPOT.TB test using manual peripheral blood mononuclear cell …
Emerging Roles And Potential Applications Of Non-Coding Rnas In Cervical Cancer, Deepak Parashar, Anupama Singh, Saurabh Gupta, Aishwarya Sharma, Manish K. Sharma, Kuldeep K. Roy, Subhash C. Chauhan, Vivek K. Kashyap
Emerging Roles And Potential Applications Of Non-Coding Rnas In Cervical Cancer, Deepak Parashar, Anupama Singh, Saurabh Gupta, Aishwarya Sharma, Manish K. Sharma, Kuldeep K. Roy, Subhash C. Chauhan, Vivek K. Kashyap
School of Medicine Publications and Presentations
Cervical cancer (CC) is a preventable disease using proven interventions, specifically prophylactic vaccination, pervasive disease screening, and treatment, but it is still the most frequently diagnosed cancer in women worldwide. Patients with advanced or metastatic CC have a very dismal prognosis and current therapeutic options are very limited. Therefore, understanding the mechanism of metastasis and discovering new therapeutic targets are crucial. New sequencing tools have given a full visualization of the human transcriptome’s composition. Non-coding RNAs (NcRNAs) perform various functions in transcriptional, translational, and post-translational processes through their interactions with proteins, RNA, and even DNA. It has been suggested that …
The Novel Proteomic Signature For Cardiac Allograft Vasculopathy, Dongmei Wei, Sander Trenson, Jan M. Van Keer, Jesus D. Melgarejo, Ella Cutsforth, Lutgarde Thijs, Tianlin He, Agnieszka Latosinska, Agnieszka Ciarka, Thomas Vanassche
The Novel Proteomic Signature For Cardiac Allograft Vasculopathy, Dongmei Wei, Sander Trenson, Jan M. Van Keer, Jesus D. Melgarejo, Ella Cutsforth, Lutgarde Thijs, Tianlin He, Agnieszka Latosinska, Agnieszka Ciarka, Thomas Vanassche
School of Medicine Publications and Presentations
Aims
Cardiac allograft vasculopathy (CAV) is the major long-term complication after heart transplantation, leading to mortality and re-transplantation. As available non-invasive biomarkers are scarce for CAV screening, we aimed to identify a proteomic signature for CAV.
Methods and results
We measured urinary proteome by capillary electrophoresis coupled with mass spectrometry in 217 heart transplantation recipients (mean age: 55.0 ± 14.4 years; women: 23.5%), including 76 (35.0%) patients with CAV diagnosed by coronary angiography. We randomly and evenly grouped participants into the derivation cohort (n = 108, mean age: 56.4 ± 13.8 years; women: 22.2%; CAV: n = 38) and …