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Full-Text Articles in Medicine and Health Sciences
Analysis Of The Psychological Impact Of A Vascular Risk Factor Intervention: Results From A Cluster Randomized Controlled Trial In Australian General Practice, Suzanne Helen Mckenzie, Upali W. Jayasinghe, Mahnaz Fanaian, Megan Passey, Mark Fort Harris
Analysis Of The Psychological Impact Of A Vascular Risk Factor Intervention: Results From A Cluster Randomized Controlled Trial In Australian General Practice, Suzanne Helen Mckenzie, Upali W. Jayasinghe, Mahnaz Fanaian, Megan Passey, Mark Fort Harris
Illawarra Health and Medical Research Institute
Background Screening for vascular disease, risk assessment and management are encouraged in general practice however there is limited evidence about the emotional impact on patients. The Health Improvement and Prevention Study evaluated the impact of a general practice-based vascular risk factor intervention on behavioural and physiological risk factors in 30 Australian practices. The primary aim of this analysis is to investigate the psychological impact of participating in the intervention arm of the trial. The secondary aim is to identify the mediating effects of changes in behavioural risk factors or BMI. Methods This study is an analysis of a secondary outcome …
Surface Analysis Of Lipids By Mass Spectrometry: More Than Just Imaging, Shane R. Ellis, Simon H. Brown, Marc In Het Panhuis, Stephen J. Blanksby, Todd W. Mitchell
Surface Analysis Of Lipids By Mass Spectrometry: More Than Just Imaging, Shane R. Ellis, Simon H. Brown, Marc In Het Panhuis, Stephen J. Blanksby, Todd W. Mitchell
Faculty of Science, Medicine and Health - Papers: part A
Mass spectrometry is now an indispensable tool for lipid analysis and is arguably the driving force in the renaissance of lipid research. In its various forms, mass spectrometry is uniquely capable of resolving the extensive compositional and structural diversity of lipids in biological systems. Furthermore, it provides the ability to accurately quantify molecular-level changes in lipid populations associated with changes in metabolism and environment; bringing lipid science to the "omics" age. The recent explosion of mass spectrometry-based surface analysis techniques is fuelling further expansion of the lipidomics field. This is evidenced by the numerous papers published on the subject of …
Food Groups And Fatty Acids Associated With Self-Reported Depression: An Analysis From The Australian National Nutrition And Health Surveys, Barbara J. Meyer, Nithin Kolanu, David A. Griffiths, Ben Grounds, Peter R. C Howe, Irene A. Kreis
Food Groups And Fatty Acids Associated With Self-Reported Depression: An Analysis From The Australian National Nutrition And Health Surveys, Barbara J. Meyer, Nithin Kolanu, David A. Griffiths, Ben Grounds, Peter R. C Howe, Irene A. Kreis
Faculty of Science, Medicine and Health - Papers: part A
Objective The aim of this study was to explore the associations between incidence of depression and dietary intakes of foods and fatty acids in adult Australians. Methods Data from the 1995 Australian National Nutrition Survey (NNS), the 1995 Australian National Health Survey (NHS) and an updated fatty acid database were merged and the 24-h fatty acid intakes were calculated for the 10 986 adult participants ages 18 to 79 y in the 1995 NNS. The merged data set was used to run a logistic regression with depression as the response variable and the food groups and calculated fatty acid values, …
Stop Thumbing Around: A Quantitative Analysis Of The Structure And Architecture Of The Transverse And Oblique Heads Of The Adductor Pollicis Muscle, A Stamenkovic, B J. Munro, G E. Peoples
Stop Thumbing Around: A Quantitative Analysis Of The Structure And Architecture Of The Transverse And Oblique Heads Of The Adductor Pollicis Muscle, A Stamenkovic, B J. Munro, G E. Peoples
Faculty of Science, Medicine and Health - Papers: part A
Abstract of paper that has been presented at 2012 Meeting of the Australian and New Zealand Association of Clinical Anatomists, University of New South Wales, Australia, 9-11 December, 2012.
An Extraction Of The Violacein Sequence From Pjp101 For Expression And Analysis In Phsg398, Julian Harrison, John Ronczka
An Extraction Of The Violacein Sequence From Pjp101 For Expression And Analysis In Phsg398, Julian Harrison, John Ronczka
Faculty of Science, Medicine and Health - Papers: part A
Violacein is a pigment found in Chromobacterium violaceum, it has a number of properties, such as; anti-bacterial, anti-viral, anti-protozoa, anti-tumoral. Violacein is produced by a series of genes named vioA, vioB, vioC, vioD and vioE. A violacein sequence is 8 kilo-bases (kb) in size. The goal of this study was to isolate the smallest possible fragment of 'Deoxyribonucleic acid' (DNA) that would produce violacein from pJP101 and making a connection to DNA biological logic gates.
Gc/Gc-Ms Analysis, Isolation And Identification Of Bioactive Essential Oil Components From The Bhutanese Medicinal Plant, Pleurospermum Amabile, Phurpa Wangchuk, Paul A. Keller, Stephen G. Pyne, Malai Taweechotipatr, Sumalee Kamchonwongpaisan
Gc/Gc-Ms Analysis, Isolation And Identification Of Bioactive Essential Oil Components From The Bhutanese Medicinal Plant, Pleurospermum Amabile, Phurpa Wangchuk, Paul A. Keller, Stephen G. Pyne, Malai Taweechotipatr, Sumalee Kamchonwongpaisan
Faculty of Science, Medicine and Health - Papers: part A
We have hydrodistilled the essential oil (EO) from the aerial parts of the Bhutanese medicinal plant, Pleurospermum amabile using a Clevenger apparatus and evaluated this EO by GC/GC-MS and NMR analysis followed by testing for bioactivity. The GC-MS analysis identified 52 compounds with (E)-isomyristicin as a major component (32.2%). Repeated purification yielded four compounds; (E)-isomyristicin (1), (E)-isoapiol (2), methyl eugenol (3) and (E)-isoelemicin (4). Compound 2 and the mother EO showed the best antiplasmodial activity against the Plasmodium falciparum strains, TM4/8.2 (chloroquine and antifolate sensitive) and K1CB1 (multidrug resistant). They exhibited mild antibacterial activity against Bacillus subtilis. None of the …
Perceptions And Practices Of Adults With Asthma: A Social Cognitive Analysis, Kelly L. Andrews, Sandra C. Jones, Judy Mullan
Perceptions And Practices Of Adults With Asthma: A Social Cognitive Analysis, Kelly L. Andrews, Sandra C. Jones, Judy Mullan
Faculty of Science, Medicine and Health - Papers: part A
This article describes a qualitative study that investigated the experiences, attitudes, and opinions of adults with asthma regarding self-managing their disease. Focus groups were conducted with 22 adults living in metropolitan and regional New South Wales, Australia. Key findings were that the perceived stigma of asthma, the need for social support, and the need for "asthma-friendly general practitioners" concerned participants more than formal self-management procedures such as written Asthma Action Plans and medication regimes. Social cognitive theory was used to explain the fluid relationship between persons with asthma, their environment, and their behavior in relation to self-management strategies and in …
An Improved High-Throughput Lipid Extraction Method For The Analysis Of Human Brain Lipids, Sarah K. Abbott, Andrew M. Jenner, Todd W. Mitchell, Simon H. Brown, Glenda M. Halliday, Brett Garner
An Improved High-Throughput Lipid Extraction Method For The Analysis Of Human Brain Lipids, Sarah K. Abbott, Andrew M. Jenner, Todd W. Mitchell, Simon H. Brown, Glenda M. Halliday, Brett Garner
Illawarra Health and Medical Research Institute
We have developed a protocol suitable for high-throughput lipidomic analysis of human brain samples. The traditional Folch extraction (using chloroform and glass-glass homogenization) was compared to a high-throughput method combining methyl-tert-butyl ether (MTBE) extraction with mechanical homogenization utilizing ceramic beads. This high-throughput method significantly reduced sample handling time and increased efficiency compared to glass-glass homogenizing. Furthermore, replacing chloroform with MTBE is safer (less carcinogenic/toxic), with lipids dissolving in the upper phase, allowing for easier pipetting and the potential for automation (i.e., robotics). Both methods were applied to the analysis of human occipital cortex. Lipid species (including ceramides, sphingomyelins, choline glycerophospholipids, …
Analysis Of Subcellular [57co] Cobalamin Distribution In Sh-Sy5y Neurons And Brain Tissue, Hua Zhao, Kalani Ruberu, Hongyun Li, Brett Garner
Analysis Of Subcellular [57co] Cobalamin Distribution In Sh-Sy5y Neurons And Brain Tissue, Hua Zhao, Kalani Ruberu, Hongyun Li, Brett Garner
Illawarra Health and Medical Research Institute
Cobalamin (Cbl) utilization as a cofactor for methionine synthase and methylmalonyl-CoA mutase is dependent on the transport of Cbl through lysosomes and its subsequent delivery to the cytosol and mitochondria. We speculated that neuropathological conditions that impair lysosomal function (e.g., age-related lipofuscinosis and specific neurodegenerative diseases) might impair lysosomal Cbl transport. To address this question, an appropriate method to quantify intracellular Cbl transport in neuronal cell types and brain tissue is required. Thus, we developed methods to measure [57Co] Cbl levels in lysosomes, mitochondria and cytosol obtained from in vitro and in vivo sources. Human SH-SY5Y neurons or …