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Expression Of Mucoid Induction Factor Muce Is Dependent Upon The Alternate Sigma Factor Algu In Pseudomonas Aeruginosa, Yeshi Yin, F. Heath Damron, T. Ryan Withers, Christopher L. Prichett, Xin Wang, Michael J. Schurr, Hongwei Yu
Expression Of Mucoid Induction Factor Muce Is Dependent Upon The Alternate Sigma Factor Algu In Pseudomonas Aeruginosa, Yeshi Yin, F. Heath Damron, T. Ryan Withers, Christopher L. Prichett, Xin Wang, Michael J. Schurr, Hongwei Yu
Biochemistry and Microbiology
Background
Alginate overproduction in P. aeruginosa, also referred to as mucoidy, is a poor prognostic marker for patients with cystic fibrosis (CF). We previously reported the construction of a unique mucoid strain which overexpresses a small envelope protein MucE leading to activation of the protease AlgW. AlgW then degrades the anti-sigma factor MucA thus releasing the alternative sigma factor AlgU/T (σ22) to initiate transcription of the alginate biosynthetic operon.
Results
In the current study, we mapped the mucE transcriptional start site, and determined that PmucE activity was dependent on AlgU. Additionally, the presence of triclosan and …