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Full-Text Articles in Medicine and Health Sciences
Rnai Therapeutics In Autoimmune Disease, Kaleb M. Pauley, Seunghee Cha
Rnai Therapeutics In Autoimmune Disease, Kaleb M. Pauley, Seunghee Cha
Science and Mathematics Faculty Publications
Since the discovery of RNA interference (RNAi), excitement has grown over its potential therapeutic uses. Targeting RNAi pathways provides a powerful tool to change biological processes post-transcriptionally in various health conditions such as cancer or autoimmune diseases. Optimum design of shRNA, siRNA, and miRNA enhances stability and specificity of RNAi-based approaches whereas it has to reduce or prevent undesirable immune responses or off-target effects. Recent advances in understanding pathogenesis of autoimmune diseases have allowed application of these tools in vitro as well as in vivo with some degree of success. Further research on the design and delivery of effectors of …
Autoantibodies Against Muscarinic Type 3 Receptor In Sjögren's Syndrome Inhibit Aquaporin 5 Trafficking, B. Lee, A. E. Gauna, G. Perez, Y. Park, Kaleb M. Pauley, Toshihisa Kawai, Seunghee Cha
Autoantibodies Against Muscarinic Type 3 Receptor In Sjögren's Syndrome Inhibit Aquaporin 5 Trafficking, B. Lee, A. E. Gauna, G. Perez, Y. Park, Kaleb M. Pauley, Toshihisa Kawai, Seunghee Cha
Science and Mathematics Faculty Publications
Sjo¨gren’s syndrome (SjS) is a chronic autoimmune disease that mainly targets the salivary and lacrimal glands. It has been controversial whether anti-muscarinic type 3 receptor (a-M3R) autoantibodies in patients with SjS inhibit intracellular trafficking of aquaporin-5 (AQP5), water transport protein, leading to secretory dysfunction. To address this issue, GFPtagged human AQP5 was overexpressed in human salivary gland cells (HSG-hAQP5) and monitored AQP5 trafficking to the plasma membrane following carbachol (CCh, M3R agonist) stimulation. AQP5 trafficking was indeed mediated by M3R stimulation, shown in partial blockage of trafficking by M3R-antagonist 4-DAMP. HSG-hAQP5 pre-incubated with SjS plasma for 24 hours significantly reduced …