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Full-Text Articles in Medicine and Health Sciences
The Effects Of Repeated Anaerobic Bouts On Immune Parameters, James C. Sivley
The Effects Of Repeated Anaerobic Bouts On Immune Parameters, James C. Sivley
Masters Theses & Specialist Projects
To date, the exercise-induced immune response in has only been evaluated in subjects performing aerobic exercise. The primary purpose of this study is to determine if repeated bouts of anaerobic exercise will induce a similar immune response in human subjects as exhibited by aerobic exercise. Secondary to immune function, an analysis of performance from trial to trial will be made. Nine males between the ages of 18-25 were selected on a volunteer basis to participate in this study. Subjects performed Wingate tests set in 3 series with 120 seconds between trials within series and 405 seconds between series for an …
Comparative Transcriptome Analyses Of Pseudomonas Aeruginosa, Deepak Balasubramanian, Kalai Mathee
Comparative Transcriptome Analyses Of Pseudomonas Aeruginosa, Deepak Balasubramanian, Kalai Mathee
HWCOM Faculty Publications
One of the hallmarks of bacterial survival is their ability to adapt rapidly to changing environmental conditions. Niche adaptation is a response to the signals received that are relayed, often to regulators that modulate gene expression. In the post-genomic era, DNA microarrays are used to study the dynamics of gene expression on a global scale. Numerous studies have used Pseudomonas aeruginosa--a Gram-negative environmental and opportunistic human pathogenic bacterium--as the model organism in whole-genome transcriptome analysis. This paper reviews the transcriptome studies that have led to immense advances in our understanding of the biology of this intractable human pathogen. Comparative …
Heme Oxygenase Activity And Hemoglobin Neurotoxicity Are Attenuated By Inhibitors Of The Mek/Erk Pathway., Jing Chen-Roetling, Zhi Li, Mai Chen, Olatilewa O. Awe, Raymond F. Regan
Heme Oxygenase Activity And Hemoglobin Neurotoxicity Are Attenuated By Inhibitors Of The Mek/Erk Pathway., Jing Chen-Roetling, Zhi Li, Mai Chen, Olatilewa O. Awe, Raymond F. Regan
Department of Emergency Medicine Faculty Papers
Hemoglobin breakdown produces an iron-dependent neuronal injury after experimental CNS hemorrhage that may be attenuated by heme oxygenase (HO) inhibitors. The HO enzymes are phosphoproteins that are activated by phosphorylation in vitro. While testing the effect of kinase inhibitors in cortical cell cultures, we observed that HO activity was consistently decreased by the MEK inhibitor U0126. The present study tested the hypothesis that MEK/ERK pathway inhibitors reduce HO activity and neuronal vulnerability to hemoglobin. The MEK inhibitors U0126 and SL327 and the ERK inhibitor FR180204 reduced baseline culture HO activity by 35-50%, without altering the activity of recombinant HO-1 or …
Hydroxyl Radical Is Produced Via The Fenton Reaction In Submitochondrial Particles Under Oxidative Stress: Implications For Diseases Associated With Iron Accumulation, Carin Thomas, Melissa M. Mackey, Amy A. Diaz, David P. Cox
Hydroxyl Radical Is Produced Via The Fenton Reaction In Submitochondrial Particles Under Oxidative Stress: Implications For Diseases Associated With Iron Accumulation, Carin Thomas, Melissa M. Mackey, Amy A. Diaz, David P. Cox
All Faculty Scholarship for the College of the Sciences
Mitochondrial dysfunction and reactive oxygen species (ROS) are often implicated in diseases involving oxidative stress and elevated iron. As mitochondria produce ATP by oxidative phosphorylation, ROS by-products are generated from the electron transport chain. Although superoxide and hydrogen peroxide have been thoroughly investigated, little evidence documents hydroxyl radical (HO•) production in mitochondria. In order to determine whether HO• is generated under oxidative stress conditions by a Fenton-type mechanism, bovine heart submitochondrial particles were examined for HO• in the presence and absence of iron ligands, antioxidant enzymes and HO• scavengers. HO• was measured as 2,3- and 2,5-dihydroxybenzoic acid (DHBA), using HPLC …