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Full-Text Articles in Medicine and Health Sciences
The Ability Of Simian Virus 40 Large T Antigen To Immortalize Primary Mouse Embryo Fibroblasts Cosegregates With Its Ability To Bind To P53., Jiyue Y. Zhu, Marina Abate, Philip W. Rice, Charles N. Cole
The Ability Of Simian Virus 40 Large T Antigen To Immortalize Primary Mouse Embryo Fibroblasts Cosegregates With Its Ability To Bind To P53., Jiyue Y. Zhu, Marina Abate, Philip W. Rice, Charles N. Cole
Dartmouth Scholarship
The large T antigen encoded by simian virus 40 (SV40) plays essential roles in the infection of permissive cells, leading to production of progeny virions, and in the infection of nonpermissive cells, leading to malignant transformation. Primary mouse embryo fibroblasts (MEFs) are nonpermissive for SV40, and infection by wild-type SV40 leads to immortalization and transformation of a small percentage of infected cells. We examined the ability of an extensive set of mutants whose lesions affect SV40 large T antigen to immortalize MEFs. We found that immortalization activity was retained by all mutants whose lesions are located upstream of codon 346. …
A Polymerase Chain Reaction-Based Method To Detect Cisplatin Adducts In Specific Genes, M M. Jennerwein, A Eastman
A Polymerase Chain Reaction-Based Method To Detect Cisplatin Adducts In Specific Genes, M M. Jennerwein, A Eastman
Dartmouth Scholarship
Every bulky lesion in DNA can potentially inhibit the Taq DNA polymerase and thereby decrease the amplification produced in the polymerase chain reaction. We investigated the feasibility of using this inhibition to quantify DNA lesions produced by the anticancer drug cisplatin. Products were detected by electrophoresis followed by ethidium bromide staining. Quantitation was obtained by including [32P]dCTP in the amplification reaction and subsequently assessing the incorporated radioactivity. Hamster genomic DNA was platinated in vitro to defined levels and amplified with primers that produce either a 150, 750 or 2,000 base pair fragment. The degree of inhibition of PCR …
Translocation Of The Glucose Transporter Glut4 In Cardiac Myocytes Of The Rat., Jan W. Slot, Hans J. Geuze, Sander Gigengack, David E. James, Gustav E. Lienhard
Translocation Of The Glucose Transporter Glut4 In Cardiac Myocytes Of The Rat., Jan W. Slot, Hans J. Geuze, Sander Gigengack, David E. James, Gustav E. Lienhard
Dartmouth Scholarship
The insulin-regulated glucose transporter GLUT4 was immunolocalized in rat cardiac muscle under conditions of basal and stimulated glucose uptake, achieved by fasting and a combined exercise/insulin stimulus, respectively. In basal myocytes there was very little (less than 1%) GLUT4 in the different domains of the plasma membrane (sarcolemma, intercalated disk, and transverse tubular system). GLUT4 was localized in small tubulo-vesicular elements that occur predominantly near the sarcolemma and the transverse tubular system and in the trans-Golgi region. Upon stimulation approximately 42% of GLUT4 was found in the plasma membrane. Each domain of the plasma membrane contributed equally to this effect. …
Evidence For Attenuation Of Myo-Inositol Uptake, Phosphoinositide Turnover And Inositol Phosphate Production In Aortic Vasculature Of Rats During Pregnancy, Kirk P. Conrad, Susan A. Barrera, Peter A. Friedman, Vicki M. Schmidt
Evidence For Attenuation Of Myo-Inositol Uptake, Phosphoinositide Turnover And Inositol Phosphate Production In Aortic Vasculature Of Rats During Pregnancy, Kirk P. Conrad, Susan A. Barrera, Peter A. Friedman, Vicki M. Schmidt
Dartmouth Scholarship
We postulated that vascular phosphoinositide metabolism is attenuated during pregnancy, and thereby could contribute to maternal vasodilation and reduced vascular reactivity. The basal rate of incorporation of [3H]myo-inositol and [3H]glycerol into phosphoinositides of aortae from pregnant rats in vitro was significantly reduced, when compared with vessels from virgin animals. After injection of [3H]myo-inositol intravenously into chronically instrumented conscious pregnant and virgin rats, the incorporation of the label by phosphatidylinositol was 66 +/- 4% less in aortae of gravid versus virgin animals (P less than 0.001), despite comparable plasma concentrations of radioactivity. Fold stimulation of total [3H]inositol phosphates by arginine vasopressin, …