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Full-Text Articles in Medicine and Health Sciences
Fetal Hemorrhage And Platelet Dysfunction In Slp-76-Deficient Mice., J. Clements, J. Lee, B. Gross, Baoli Yang, J. Olson, A. Sandra, S. Watson, S. Lentz, G. Koretzky
Fetal Hemorrhage And Platelet Dysfunction In Slp-76-Deficient Mice., J. Clements, J. Lee, B. Gross, Baoli Yang, J. Olson, A. Sandra, S. Watson, S. Lentz, G. Koretzky
Baoli Yang
The adapter protein SLP-76 is expressed in T lymphocytes and hematopoietic cells of the myeloid lineage, and is known to be a substrate of the protein tyrosine kinases that are activated after ligation of the T-cell antigen receptor. Transient overexpression of SLP-76 in a T-cell line potentiates transcriptional activation after T-cell receptor ligation, while loss of SLP-76 expression abrogates several T-cell receptor-dependent signaling pathways. Mutant mice that lack SLP-76 manifest a severe block at an early stage of thymocyte development, implicating SLP-76 in signaling events that promote thymocyte maturation. While it is clear that SLP-76 plays a key role in …
Parameters For Establishing Humanized Mouse Models To Study Human Immunity: Analysis Of Human Hematopoietic Stem Cell Engraftment In Three Immunodeficient Strains Of Mice Bearing The Il2rgamma(Null) Mutation, Michael Brehm, Amy Cuthbert, Chaoxing Yang, David Miller, Philip Diiorio, Joseph Laning, Lisa Burzenski, Bruce Gott, Oded Foreman, Anoop Kavirayani, Mary Herlihy, Aldo Rossini, Leonard Shultz, Dale Greiner
Parameters For Establishing Humanized Mouse Models To Study Human Immunity: Analysis Of Human Hematopoietic Stem Cell Engraftment In Three Immunodeficient Strains Of Mice Bearing The Il2rgamma(Null) Mutation, Michael Brehm, Amy Cuthbert, Chaoxing Yang, David Miller, Philip Diiorio, Joseph Laning, Lisa Burzenski, Bruce Gott, Oded Foreman, Anoop Kavirayani, Mary Herlihy, Aldo Rossini, Leonard Shultz, Dale Greiner
Philip J diIorio Jr
"Humanized" mouse models created by engraftment of immunodeficient mice with human hematolymphoid cells or tissues are an emerging technology with broad appeal across multiple biomedical disciplines. However, investigators wishing to utilize humanized mice with engrafted functional human immune systems are faced with a myriad of variables to consider. In this study, we analyze HSC engraftment methodologies using three immunodeficient mouse strains harboring the IL2rgamma(null) mutation; NOD-scid IL2rgamma(null), NOD-Rag1(null) IL2rgamma(null), and BALB/c-Rag1(null) IL2rgamma(null) mice. Strategies compared engraftment of human HSC derived from umbilical cord blood following intravenous injection into adult mice and intracardiac and intrahepatic injection into newborn mice. We observed …