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Comparison Of Milk Microbiota Between Healthy And Mastitic Cows, Sibel Kizil, Fatma Esin Aydin, Cansu Önlen Güneri̇, Ufuk Ülker, Gürol Emekdaş, İbrahim Basmaci, Serkan Erat, Rıza Durmaz, Efsun Melike Çeçen, Asya Kazan Feb 2024

Comparison Of Milk Microbiota Between Healthy And Mastitic Cows, Sibel Kizil, Fatma Esin Aydin, Cansu Önlen Güneri̇, Ufuk Ülker, Gürol Emekdaş, İbrahim Basmaci, Serkan Erat, Rıza Durmaz, Efsun Melike Çeçen, Asya Kazan

Turkish Journal of Veterinary & Animal Sciences

Mammary gland infections occur due to bacterial changes in the mammary tissue. Studies conducted in recent years have reported variations in the most common bacteria differ according to geographical locations. California mastitis test (CMT), somatic cell count (SCC), and aerobic colony count (ACC) analyses were performed on approximately 50 mL of hygienically collected raw milk samples. Raw milk was also subjected to conventional bacteriological isolation and identification. Bacterial diversity and rates in raw milk were compared through metagenome analysis. Two samples, one from healthy milk and another from subclinical milk with mastitis, were independently tested to determine whether there were …


Prevalence And Risk Factors Of Post-Partum Infections At Family Health Facilities In North Sinai, Egypt, Aml Ahmed Salama, Samah Abduallah Salim, Safa Hamdy Alkalash Jan 2024

Prevalence And Risk Factors Of Post-Partum Infections At Family Health Facilities In North Sinai, Egypt, Aml Ahmed Salama, Samah Abduallah Salim, Safa Hamdy Alkalash

Menoufia Medical Journal

Objectives: To assess the prevalence of different types of postpartum infections and their risk factors among women attending family health facilities in Bir El-Abd district, North Sinai, Egypt. Background: The postpartum period is an imminent period among women; infection in this critical period is a preventable cause of maternal morbidity and mortality. Methods: An analytical cross-sectional study was conducted among a random sample of 220 women attending primary health facilities in Bir El-Abd district, North Sinai, during their postpartum period. They were assessed through a complete history taken via a pre-designed questionnaire. Complete general and local examinations were done through …


Determination Of Virulence Genes And Antibiotic Resistance Profiles Of Streptococcus Agalactiae Isolated From Buffalo Milk, Nevi̇n Tuzcu, Hasan Hüseyi̇n Hadi̇mli̇, Beatri̇z Padron Aug 2023

Determination Of Virulence Genes And Antibiotic Resistance Profiles Of Streptococcus Agalactiae Isolated From Buffalo Milk, Nevi̇n Tuzcu, Hasan Hüseyi̇n Hadi̇mli̇, Beatri̇z Padron

Turkish Journal of Veterinary & Animal Sciences

Mastitis is a contagious disease that causes significant milk loss and serious economic damage in dairy animals. Streptococcus agalactiae causes clinical and subclinical mastitis in animals and infections in humans, especially adults, and newborns. This study aimed to confirm the identification of S. agalactiae isolates by PCR in buffalo milk; to determine the antibiotic resistance and virulence genes by multiplex PCR. The material of this study consisted of 30 (12%) S. agalactiae strains identified by phenotypic, biochemical, and PCR methods (rRNA-16S) from 250 isolates with bacterial growth from milk samples of buffaloes with mastitis. In addition, the Kirby-Bauer disc diffusion …


Investigations On Some Aerobic Bacterial Agents Isolated From Heifers At The Beginning Ofthe First Lactation, Tuncay Çökülgen, Uçkun Sai̇t Uçan Jan 2022

Investigations On Some Aerobic Bacterial Agents Isolated From Heifers At The Beginning Ofthe First Lactation, Tuncay Çökülgen, Uçkun Sai̇t Uçan

Turkish Journal of Veterinary & Animal Sciences

In this study, it was aimed to identify the predominant coagulase-negative staphylococci (CNS) species in subclinical, clinical, and persistent infections in Konya province, to determine various virulence and resistance mechanisms of CNS species within the agelactation level. The research has been carried out in a dairy herd that is free from brucellosis and tuberculosis and uses an automated herd management system. Thirty-two heifers at the beginning of lactation with milk yields of more than 20 liters have been observed in terms of udder infections during the first 1-4 months of lactation. The prevalence of intramammary infections (IMI) and of CNS-caused …


A Comparative Study Of Blood And Milk Chemiluminescence In Healthy Cows And Withsubclinical And Clinical Mastitis, Radoslaw Skorzewski, Wieslaw Krumrych, Hanna Markiewicz, Elzbieta Sandurska Jan 2022

A Comparative Study Of Blood And Milk Chemiluminescence In Healthy Cows And Withsubclinical And Clinical Mastitis, Radoslaw Skorzewski, Wieslaw Krumrych, Hanna Markiewicz, Elzbieta Sandurska

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to evaluate the luminol-dependent spontaneous and stimulated chemiluminescence (CL) of whole blood and milk in cows with subclinical and clinical mastitis. The research was carried out on 108 Holstein-Friesian dairy cows aged 3-5 years. Based on the assessment of somatic cell count (SCC) in quarter milk, the animals were divided into 3 groups: I - clinically healthy cows (n = 35), II - animals with subclinical mastitis (n = 30) and III - cows with clinical mastitis (n = 43). The material for the research was whole blood and quarter milk. The samples were …


Association Of Snps In The Coding Regions Of Cd4 Gene With Mastitis Susceptibility And Production Traits In Dairy Cattle, Saqib Zeb, Nawab Ali, Sadaf Niaz, Arsalan Rasheed, Irfan Khattak, Naimat Ullah Khan, Yachun Wang, Tahir Usman Mar 2020

Association Of Snps In The Coding Regions Of Cd4 Gene With Mastitis Susceptibility And Production Traits In Dairy Cattle, Saqib Zeb, Nawab Ali, Sadaf Niaz, Arsalan Rasheed, Irfan Khattak, Naimat Ullah Khan, Yachun Wang, Tahir Usman

The Thai Journal of Veterinary Medicine

CD4 gene is reported to have significant role in various inflammatory conditions including mastitis. Therefore, the current study was designed to screen out single nucleotide polymorphisms (SNPs) in the bovine CD4 gene and to evaluate its effects on mastitis indicator and production traits. Total 201 milk and blood samples were collected from dairy cattle maintained at government dairy farms of Khyber Pakhtunkhwa, Pakistan. Milk samples were used for DHI test (milk composition and somatic cell count) and the blood for DNA extraction. SNPs were initially detected in the pool DNA samples followed by screening in the whole cohort. The association …


Application Of An Indirect Mila Elisa For The Detection Of Mycoplasma Bovis Antibodiesin Bovine Milk, Abd Al Bar Al Farha, Nadeeka Wawegama, Farhid Hemmatzadeh, Simon Firestone, Jamie Moffat, Gholam Ali Kojouri, Ania Ahaniazari, Reza Amanollahi, Andrew Hoare, Kiro Petrovski Jan 2020

Application Of An Indirect Mila Elisa For The Detection Of Mycoplasma Bovis Antibodiesin Bovine Milk, Abd Al Bar Al Farha, Nadeeka Wawegama, Farhid Hemmatzadeh, Simon Firestone, Jamie Moffat, Gholam Ali Kojouri, Ania Ahaniazari, Reza Amanollahi, Andrew Hoare, Kiro Petrovski

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to detect Mycoplasma bovis specific antibodies using a recently reported MilA ELISA with the aim to detect M. bovis antibodies in milk. An indirect ELISA, based on a recombinant fragment of the Mycoplasma immunogenic lipase A (MilA) protein, was conducted on 291 milk samples for the detection of M. bovis antibodies. Samples were also tested with conventional Mycoplasma culture and M. bovis PCR. Samples were collected from individual cows from 2 commercial dairy herds in South Australia. Of 291 samples tested, 68 (23.4%) were detected positive for M. bovis antibodies, 150 (51.5%) were positive …


Screening Antimicrobial Properties Against Mastitis Pathogens Of Turmeric Extract After Combination With Various Antiseptics, Wasana Chaisri, Noppason Pangprasit, Anyaphat Srithanasuwan, Montira Intanon, Witaya Suriyasathaporn Sep 2019

Screening Antimicrobial Properties Against Mastitis Pathogens Of Turmeric Extract After Combination With Various Antiseptics, Wasana Chaisri, Noppason Pangprasit, Anyaphat Srithanasuwan, Montira Intanon, Witaya Suriyasathaporn

The Thai Journal of Veterinary Medicine

The aim of this study was to determine the screening for antimicrobial properties against mastitis pathogens of turmeric extract in combination with various antiseptics. The antibacterial effects of turmeric extract in combination with various antiseptics (5% povidone iodine, 0.5% v/v hydrogen peroxide (H2O2), 0.5% v/v chlorine (Cl2), and 0.5% v/v chlorhexidine) were determined using the agar welldiffusion method. Results showed that additional turmeric extract had significantly decreased the antimicrobial activities of either Cl2or chlorhexidine against almost all mastitis pathogens, except S. agalactiaefor Cl2 and gram negative bacteria for chlorhexidine. In contrast, no negative effect was found between turmeric extract and …


Identification Of Streptococcus Spp. Isolated From Bovine Milk And Characterization Of Their Antimicrobial Susceptibility Profiles In Taiwan, Jui-Chun Hsieh, Yu-Shan Yen, Shih-Te Chuang Mar 2019

Identification Of Streptococcus Spp. Isolated From Bovine Milk And Characterization Of Their Antimicrobial Susceptibility Profiles In Taiwan, Jui-Chun Hsieh, Yu-Shan Yen, Shih-Te Chuang

The Thai Journal of Veterinary Medicine

Bovine mastitis has had a great impact on the economic losses of the dairy industry. Nowadays, environmental pathogens cause the main types of bovine mastitis, especially the streptococci. The aim of the study was to identify the major Streptococcus species causing bovine mastitis in Taiwan as well as to reveal their antimicrobial susceptibility profiles. By colony morphology identifying, Gram staining, and subculturing on Edwards agar, the preliminary identified streptococci were subjected to biochemical identification, latex agglutination tests and dick diffusion tests for susceptibility tests of 16 antimicrobial agents. Among a total of 157 strains, Streptococcus uberis was the most dominant …


Study Of Mastitis Comorbidities Among Lactating Mothers In El-Batanoon Village, Menoufia Governorate, Hala M. Shahin, Heba A. Elgohary Taman, Nagwaa N. Hegazy Mar 2019

Study Of Mastitis Comorbidities Among Lactating Mothers In El-Batanoon Village, Menoufia Governorate, Hala M. Shahin, Heba A. Elgohary Taman, Nagwaa N. Hegazy

Menoufia Medical Journal

Objective This study aimed to deter mine the prevalence of mastitis among lactating mothers in El-Batanoon and to assess the risk factors of mastitis. Background Mastitis is one of the most common frequent breastfeeding problems among lactating mothers and is frequently associated with comorbid illnesses. Patients and methods A case–control study of breastfeeding women, 45 patients (women with mastitis), and 355 control participants was carried out in El-Batanoon family health facilities. All the women attending the vaccination sessions for the children in the family health center on Mondays from 9 a.m. to 2 p.m. for 6 months were assessed. Data …


Identification Of Polymorphism In The Bovine Interleukin-17a Gene And Its Association With Mastitis In Polish Holstein?Friesian Cattle, Magdalena Dusza, Joanna Pokorska, Dominika Ku?Aj, Joanna Makulska Jan 2018

Identification Of Polymorphism In The Bovine Interleukin-17a Gene And Its Association With Mastitis In Polish Holstein?Friesian Cattle, Magdalena Dusza, Joanna Pokorska, Dominika Ku?Aj, Joanna Makulska

Turkish Journal of Veterinary & Animal Sciences

The main aim of this study was to identify the association between the single nucleotide polymorphism (SNP) in the bovine interleukin-17A (IL-17A) gene and mastitis resistance. Additionally, the connection between the identified polymorphism and the somatic cell score (SCS) was tested. Two hundred and six Polish Holstein?Friesian cows were genotyped using the PCR-RFLP method. One SNP mutation (c.126G>A) was identified within the coding sequence of the analyzed gene. Results of logistic regression analysis displayed no significant association between the IL-17A genotype and clinical mastitis occurrence (Wald chi-square test = 0.74; P = 0.69). The GLM analysis demonstrated that genotype …


Investigation Into Antimicrobial Resistance, Enterotoxin And Cassette Chromosome Gene Of Staphylococcus Aureus Isolates From Humans, Cows And Goats In Taiwan, Chi-Shih Chu, Han Hsiang Huang, Shou-Heng Chiang, Chi-Chung Chou, Jyh-Mirn Lai, Wen-Ling Shih, Chih-Hsuan Changchien, Heng-Ching Lin, Shih-Te Chuang, Yao-Chi Su Dec 2017

Investigation Into Antimicrobial Resistance, Enterotoxin And Cassette Chromosome Gene Of Staphylococcus Aureus Isolates From Humans, Cows And Goats In Taiwan, Chi-Shih Chu, Han Hsiang Huang, Shou-Heng Chiang, Chi-Chung Chou, Jyh-Mirn Lai, Wen-Ling Shih, Chih-Hsuan Changchien, Heng-Ching Lin, Shih-Te Chuang, Yao-Chi Su

The Thai Journal of Veterinary Medicine

Multidrug-resistant Staphylococcus aureus (MDR S. aureus) is predominately present in humans and animals. Staphylococcal enterotoxin (SE) is one of the representative toxins produced by S. aureus. The drug resistance patterns, presence of eighteen SE genes and staphylococcal cassette chromosome (SCCmec) in S. aureus strains isolated from human cellulitis, septicemia, mastitis and farmed ruminant mastitis were investigated and analyzed. Methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) accounted for 51.35% (76/148) and 48.65% (72/148), respectively. Both MSSA and MRSA carried sea (15%), seb (30.8%) and sek (27.5%) genes. Eighty percent of the MRSA strains (52/65) belonged to SCCmec types I, …


Occurrence Of Mastitis And Associated Pathogens With Antibiogram In Animal Population Of Peshawar, Pakistan, Rafiullah Rafiullah, Mirza Ali Khan, Muhammad Shafee, Ali Akbar, Anwar Ali, Muhammad Shoaib, Faiza Ashraf, Naimatullah Khan Mar 2017

Occurrence Of Mastitis And Associated Pathogens With Antibiogram In Animal Population Of Peshawar, Pakistan, Rafiullah Rafiullah, Mirza Ali Khan, Muhammad Shafee, Ali Akbar, Anwar Ali, Muhammad Shoaib, Faiza Ashraf, Naimatullah Khan

The Thai Journal of Veterinary Medicine

Mastitis is a devastating disease of dairy animals in Pakistan that occurs in clinical and subclinical forms. Various bacterial pathogens are associated with mastitis and the susceptibility of these pathogens remains inconsistent to various antibacterial drugs. This study was aimed to assess the prevalence of mastitis in and around the city of Peshawar, Pakistan. A total of 2,791 milk samples (Bovine, Ovine and Caprine) were subjected to White side test and Surf field mastitis test. Positive milk samples for mastitis were cultured on different selective culture media for identification of prevalent bacterial pathogens. Antibiogram of these organisms was evaluated against …


Identification Of Candida Strains With Nested Pcr In Bovinemastitis And Determination Of Antifungal Susceptibilities, Göksel Erbaş, Uğur Parin, Şükrü Kirkan, Serap Savaşan, Volkan Özavci, Hafi̇ze Tuğba Yüksel Jan 2017

Identification Of Candida Strains With Nested Pcr In Bovinemastitis And Determination Of Antifungal Susceptibilities, Göksel Erbaş, Uğur Parin, Şükrü Kirkan, Serap Savaşan, Volkan Özavci, Hafi̇ze Tuğba Yüksel

Turkish Journal of Veterinary & Animal Sciences

Mastitis is one of the biggest problems of dairy cattle industries. In recent years, fungal agents have been frequently reported among the causative agents for mastitis. Among the fungal agents, Candida species are the most common. This study's aims were the isolation, identification, and determination of antifungal susceptibility of Candida species causing mycotic mastitis in cattle. A total of 260 mastitic milk samples were collected from different farms. Identification was conducted by rapid diagnostic tests and nested PCR method; 6 different antifungal agents were examined. Candida sp. was detected in 46 (17.7%) of the 260 mastitic milk samples. Based on …


Evaluation Of Immunotherapeutic Potential Of Ocimum Sanctumin Bovine Subclinical Mastitis, Tawheed Ahmad Shafi, Baljinder Kumar Bansal, Dhiraj Kumar Gupta, Shashi Nayyar Jan 2016

Evaluation Of Immunotherapeutic Potential Of Ocimum Sanctumin Bovine Subclinical Mastitis, Tawheed Ahmad Shafi, Baljinder Kumar Bansal, Dhiraj Kumar Gupta, Shashi Nayyar

Turkish Journal of Veterinary & Animal Sciences

Mastitis, the inflammation of mammary glands, is caused by a wide range of contagious and environmental microbes, whose clearance from the udder is generally dependent on the use of antibiotics. However, antibiotic therapy may result in development of drug-resistant microbes and depress the activity of polymorphonuclear cells. The present study was therefore undertaken to explore the immunotherapeutic potential of an herb, Ocimum sanctum, in mastitis in dairy cows. The study involved twenty HF × Sahiwal lactating dairy cows, each identified with at least one specific subclinical mastitis quarter as per International Dairy Federation criteria. The cows were divided into two …


Herd-Specific Autovaccine And Antibiotic Treatment In Elimination Of Staphylococcus Aureus Mastitis In Dairy Cattle, Danuta Czernomysy-Furowicz, Karol Fijalkowski, Anna Silecka, Jolanta Karakulska, Pawel Nawrotek, Radoslaw Drozd, Magdalena Ferlas, Jacek Borkowski, Dorota Jankowiak Jan 2014

Herd-Specific Autovaccine And Antibiotic Treatment In Elimination Of Staphylococcus Aureus Mastitis In Dairy Cattle, Danuta Czernomysy-Furowicz, Karol Fijalkowski, Anna Silecka, Jolanta Karakulska, Pawel Nawrotek, Radoslaw Drozd, Magdalena Ferlas, Jacek Borkowski, Dorota Jankowiak

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to compare the effectiveness of cefuroxime, herd-specific autovaccine, and cefuroxime/herd-specific autovaccine treatments in eliminating S. aureus from the milk of 45 cows with subclinical mastitis. Prior to this study, cows with S. aureus mastitis were divided into 3 groups of 15 cows each. Group 1 received cefuroxime, group 2 received the autovaccine, and group 3 received cefuroxime and the autovaccine. The antibiotic was infused into the teat canal, whereas the autovaccine was administrated in the upper udder lymph node region. It was revealed that after day 35 of the treatment S. aureus was …


Determination Of Plasma Fibrinogen And Haptoglobin, Hematological And Blood Biochemical Changes In Bulgarian Local Goats With Experimentally Induced Staphylococcus Aureus Mastitis, Ivan Fasulkov, Manol Karadaev, Nasko Vasilev, Valentina Urumova, Teodora Mircheva Jan 2014

Determination Of Plasma Fibrinogen And Haptoglobin, Hematological And Blood Biochemical Changes In Bulgarian Local Goats With Experimentally Induced Staphylococcus Aureus Mastitis, Ivan Fasulkov, Manol Karadaev, Nasko Vasilev, Valentina Urumova, Teodora Mircheva

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to assess changes in acute phase proteins fibrinogen and haptoglobin, as well as hematological, blood biochemical, and microbiological alterations in local Bulgarian goats with experimentally induced Staphylococcus aureus mastitis. The experiments were conducted with 6 clinically healthy local Bulgarian goats, 2 to 6 years of age, weighing 46-57 kg. The experimental infection was done with a stationary phase culture of a field Staphylococcus aureus strain isolated from a cow with clinical mastitis. Blood haptoglobin in goats was considerably higher (P < 0.05) as early as 8 h after the pathogen's inoculation. The most significant differences from baseline values occurred 24 and 48 h (P < 0.01) after infection. Plasma fibrinogen increased significantly (P < 0.05) 8 h after infection and reached the highest mean concentration (9.12 g/L) by 72 h. Leukocytosis was established as early as 4 h after the experimental infection when leukocyte counts were significantly higher than baseline (P < 0.001). The analysis of results indicates that in experimentally induced Staphylococcus aureus mastitis, significantly higher (P < 0.05) levels of the leukocyte, fibrinogen, and haptoglobin are established as early as 8 h after the infection.


Investigation Of The Efficacy Of Tarantula Cubensis Extract (Theranekron D6) In The Treatment Of Subclinical And Clinical Mastitis In Dairy Cows, Kutlay Gürbulak, Aytaç Akçay, Kadi̇r Semi̇h Gümüşsoy, Birgit Sist, Simone Steiner, Murat Abay, Esra Canooğlu, Tayfur Bekyürek Jan 2014

Investigation Of The Efficacy Of Tarantula Cubensis Extract (Theranekron D6) In The Treatment Of Subclinical And Clinical Mastitis In Dairy Cows, Kutlay Gürbulak, Aytaç Akçay, Kadi̇r Semi̇h Gümüşsoy, Birgit Sist, Simone Steiner, Murat Abay, Esra Canooğlu, Tayfur Bekyürek

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the effects of the use of Theranekron alone or in combination with intramammary antibiotic therapy for the treatment of subclinical and clinical mastitis on the recovery rate from mastitis. The study material was composed of 177 mammary lobes of 63 Holstein cows with subclinical (groups S1 and S2) and clinical mastitis (groups C1, C2, C3, and C4). In all of the treatment groups, excluding group S2, the California mastitis test scores were determined to have decreased after treatment (P < 0.05). In groups C1, C2, C3, and C4, which were treated for clinical mastitis, the somatic cell count (SCC) values were observed to have decreased. The decrease in SCC values was statistically significant except in group C2 (P < 0.01). While the SCC values were observed to have decreased in group S1 (P > 0.05), which was treated with Theranekron alone, the same values were found to …


Susceptibility Of Prototheca Zopfii Strains Isolated From Cows With Mastitis To Chlorhexidine And Iodine, Henryk Krukowski, Andrzej Lisowski, Bozena Nowakowicz-Debek, Lukasz Wlazlo Jan 2013

Susceptibility Of Prototheca Zopfii Strains Isolated From Cows With Mastitis To Chlorhexidine And Iodine, Henryk Krukowski, Andrzej Lisowski, Bozena Nowakowicz-Debek, Lukasz Wlazlo

Turkish Journal of Veterinary & Animal Sciences

Bovine mastitis caused by Prototheca spp. can assume high significance because of economic losses and the potential risk to public health. The aim of our study was to evaluate the effect of chlorhexidine and iodine on Prototheca zopfii strains isolated from bovine milk. For this study, 15 Prototheca zopfii isolates previously obtained from cows with clinical and subclinical mastitis in Poland were used. The in vitro susceptibility tests to chlorhexidine and iodine of the strains of Prototheca zopfii for the determination of their minimal microbicidal concentrations (MMCs) were performed using the tube dilution method in Sabouraud dextrose broth and the …


Chronic Mastitis In Small Dairy Cattle Herds In Muang Khon Kaen, Jaruwan Kampa, Varaporn Sukolapong, Anantachai Chaiyotwittakun, Sarinya Rerk-U-Suke, Arunee Polpakdee Sep 2010

Chronic Mastitis In Small Dairy Cattle Herds In Muang Khon Kaen, Jaruwan Kampa, Varaporn Sukolapong, Anantachai Chaiyotwittakun, Sarinya Rerk-U-Suke, Arunee Polpakdee

The Thai Journal of Veterinary Medicine

Monthly records of bulk tank milk somatic cell count (BMSCC) of all 194 dairy herds in Muang, Khon Kaen were used as an indicator of chronic mastitis problems; i.e. BMSCC > 500,000 cells/ml at least 3 consecutive records. Twelve chronic mastitis herds in Muang, Khon Kaen were data collected on 1) general herd information, 2) milking equipment, 3) milking procedure and 4) awareness and management of farmers to the mastitis problem. All possible mastitis pathogens were investigated in individual milk samples from 138 lactating cows of the herds. At least 40% of dairy herds in Muang, Khon Kaen had mastitis at …


Prevalence Of Mycoplasma Bovis And Other Contagious Bovine Mastitis Pathogens In Bulk Tank Milk Of Dairy Cattle Herds In Khon Kaen Province, Thailand, Jaruwan Kampa, Varaporn Sukolapong, Arunee Buttasri, Apirom Charoenchai Sep 2009

Prevalence Of Mycoplasma Bovis And Other Contagious Bovine Mastitis Pathogens In Bulk Tank Milk Of Dairy Cattle Herds In Khon Kaen Province, Thailand, Jaruwan Kampa, Varaporn Sukolapong, Arunee Buttasri, Apirom Charoenchai

The Thai Journal of Veterinary Medicine

Mastitis is a most frequent and costly disease of dairy cattle worldwide. All three contagious mastitis pathogens, Mycoplasma bovis, Streptococcus agalactiae and Staphylococcus aureus were investigated in 55 bulk tank milk samples from dairy cattle herds in Khon Kaen Province Thailand, by nested PCR and/or conventional bacterial culture. Bulk milk somatic cell count (BMSCC) was used as indicator for mastitis problem; i.e. > 5x105 somatic cells/ml of milk. The prevalence of Mycoplasma bovis, Streptococcus agalactiae and Staphylococcus aureus were 1.8%, 21.8% and 7.3%, respectively. Results from BMSCC indicated mastitis problem in 47 herds. However, 34 high-BMSCC with negative-contagious pathogen identification samples …


Characterisation Of Coagulase Positive Staphylococcus Species Isolated From Bovine Mastitis Using Protein And Plasmid Patterns, Emi̇ne Arslan, Ayten Çelebi̇, Leyla Açik, Uçkun Sai̇t Uçan Jan 2009

Characterisation Of Coagulase Positive Staphylococcus Species Isolated From Bovine Mastitis Using Protein And Plasmid Patterns, Emi̇ne Arslan, Ayten Çelebi̇, Leyla Açik, Uçkun Sai̇t Uçan

Turkish Journal of Veterinary & Animal Sciences

The objective of this work was to study the protein patterns, plasmid profiles, and antibiotic susceptibility of Staphylococcus intermedius and Staphylococcus aureus isolates originating from mastitic mammary glands of dairy cattle in different parts of Konya province. A total of 114 Staphylococcus species were isolated and identified by conventional bacteriological methods from bovine mastitis. Of the total isolates 77 were identified as S. aureus and 37 as S. intermedius. Intra- and inter-species diversities in the coagulase-positive staphylococci were investigated by analysis of whole-cell protein profiles using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Plasmid profiling also demonstrated that 75 S. aureus …


Investigation Of The Incidence And Diagnosis Of Subclinical Mastitis In Early Lactation Period Cows, Ayhan Baştan, Ci̇han Kaçar, Duygu B. Acar, Mi̇that Şahi̇n, Mehmet Cengi̇z Jan 2008

Investigation Of The Incidence And Diagnosis Of Subclinical Mastitis In Early Lactation Period Cows, Ayhan Baştan, Ci̇han Kaçar, Duygu B. Acar, Mi̇that Şahi̇n, Mehmet Cengi̇z

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to compare the results of the California mastitis test (CMT) and bacteriological culture for detection of subclinical intramammary infections after 10 days postpartum. Samples were collected from 102 cows. The CMT was performed once on each cow. The results of bacteriological culture and the CMT were compared in 344 milk samples. Two hundred fifty samples were CMT (+) and 94 samples were CMT (-); 224 samples were bacteriologic culture (+) and 120 samples were culture (-). Both CMT and culture (+) samples numbered 212, CMT and culture (-) samples 82, CMT (+) and culture …


Disseminated Streptococcus Equi Subsp. Zooepidemicus Infection In A Foal And Associated Mastitis In A Mare, A. Levent Kocabiyik, Esra Büyükcangaz, Ahmet Akkoç, Cüneyt Özakin, İ. Taci̇ Cangül Jan 2008

Disseminated Streptococcus Equi Subsp. Zooepidemicus Infection In A Foal And Associated Mastitis In A Mare, A. Levent Kocabiyik, Esra Büyükcangaz, Ahmet Akkoç, Cüneyt Özakin, İ. Taci̇ Cangül

Turkish Journal of Veterinary & Animal Sciences

A systemic infection with Streptococcus equi subsp. zooepidemicus is described in a 22-day-old female foal for the first time in Turkey. Clinically the foal was in poor condition and had severe pneumonia. Painful swellings of the carpal and tarsal joints were evident. Examination of the dam revealed clinical mastitis. Postmortem histopathological and microbiological examinations of samples from the foal revealed a disseminated infection with S. equi subsp. zooepidemicus. The causative agent was also recovered in pure culture from a milk sample of the mare.


Coagulase Gene Polymorphism Of Staphylococcus Aureus Isolated From Subclinical Bovine Mastitis, Özkan Aslantaş, Cemi̇l Demi̇r, Hülya Türütoğlu, Zafer Canteki̇n, Yaşar Ergün, Gökhan Doğruer Jan 2007

Coagulase Gene Polymorphism Of Staphylococcus Aureus Isolated From Subclinical Bovine Mastitis, Özkan Aslantaş, Cemi̇l Demi̇r, Hülya Türütoğlu, Zafer Canteki̇n, Yaşar Ergün, Gökhan Doğruer

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitic milk samples from 3 different Turkish cities (Hatay, Gaziantep, and Burdur). Amplification of the coagulase gene from 80 S. aureus isolates produced 5 different polymerase chain reaction (PCR) products; 79 isolates showed only 1 amplicon, and 1 isolate showed 2 amplicons. The isolates were grouped into 9 genotypes by analysing the restriction fragment length polymorphism (RFLP) of the gene; the 2 most common genotypes accounted for 73.8% of the isolates. The results indicated that many coagulase gene genotypes are present in the …


Plasmids And Protein Patterns Of Escherichia Coli Isolated From Bovine Mastitis In Konya, Turkey, Uçkun Sai̇t Uçan, Leyla Açik, Ayten Çelebi̇, Osman Ergani̇ş, Emi̇ne Arslan Jan 2005

Plasmids And Protein Patterns Of Escherichia Coli Isolated From Bovine Mastitis In Konya, Turkey, Uçkun Sai̇t Uçan, Leyla Açik, Ayten Çelebi̇, Osman Ergani̇ş, Emi̇ne Arslan

Turkish Journal of Veterinary & Animal Sciences

In this study, a total of 30 Escherichia coli isolates obtained from milk samples of dairy cows suffering from subclinical mastitis in Konya, Turkey were typed according to plasmids and protein patterns. Agarose gel electrophoresis and sodium doedecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) methods were used to identify plasmids and whole-cell protein profiles. Of these two methods, SDS-PAGE typing proved to be more discriminate for typing the isolates.


Identification And Antimicrobial Susceptibility Of Staphylococcus Aureus And Coagulase Negative Staphylococci From Bovine Mastitis In The Aydın Region Of Turkey, Şükrü Kirkan, Ergün Ö. Göksoy, Osman Kaya Jan 2005

Identification And Antimicrobial Susceptibility Of Staphylococcus Aureus And Coagulase Negative Staphylococci From Bovine Mastitis In The Aydın Region Of Turkey, Şükrü Kirkan, Ergün Ö. Göksoy, Osman Kaya

Turkish Journal of Veterinary & Animal Sciences

Three hundred bovine milk samples with mastitis were used. Eighty-five (28.33%) strains were identified as Staphylococcus aureus whereas 60 (20.00%) strains were isolated as coagulase negative staphylococcus. Bacteriological cultures of coagulase negative staphylococcus were identified as 20 (33.33%) strains of S. hyicus, 16 (26.66%) strains of S. chromogenes, 9 (15.00%) strains of S. epidermidis, 5 (8.33%) strains of S. haemolyticus, 4 (6.66%) strains of S. sciuri, 3 (5.00%) strains of S. lentis and 3 (5.00%) strains of S. cohnii subsp. cohnii. The antibiotic susceptibility test results showed that Staphylococcus aureus strains were susceptible to ciprofloxacin (100.00%), neomycin + bacitracin + …


Bacteriological Investigation Of Udder Lobes Of Cows With Mastitis Slaughtered In The Elazığ Region, Hasan Basri̇ Gülcü, Hasan Basri̇ Ertaş Jan 2004

Bacteriological Investigation Of Udder Lobes Of Cows With Mastitis Slaughtered In The Elazığ Region, Hasan Basri̇ Gülcü, Hasan Basri̇ Ertaş

Turkish Journal of Veterinary & Animal Sciences

Bacteriological examinations were performed on 206 mammary tissue samples taken from 160 cows with mastitis in Elazığ. The isolates were identified as Staphylococcus aureus (39.04%), Staphylococcus epidermidis (17.81%), Actinomyces pyogenes (14.38%), Escherichia coli (8.9%), Streptococcus agalactiae (6.16%), Streptococcus uberis (4.11%), Streptococcus dysgalactiae (3.42%), Corynebacterium bovis (1.37%), Pasteurella haemolytica (1.37%) and Bacillus cereus (1.37%).


Cost Of Mastitis In Scottish Dairy Herds With Low And High Subclinical Mastitis Problems, Cengi̇z Yalçin Jan 2000

Cost Of Mastitis In Scottish Dairy Herds With Low And High Subclinical Mastitis Problems, Cengi̇z Yalçin

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to estimate the cost of mastitis and the contribution of each cost component of mastitis to the total mastitis induced cost in herds with low and high levels of subclinical mastitis under Scottish field conditions. It was estimated that mastitis cost £140 per cow/year to the average Scottish dairy farmer in 1996. However, this figure was as low as £69 per cow/year in herds with lower levels of subclinical mastitis, and as high as £228 cow/year in herds with high sub- clinical mastitis. The magnitude and proportion of the cost components in the total …


Effects Of Mastitis On Milk Yield And Composition, Department Of Agriculture, Western Australia Jan 1970

Effects Of Mastitis On Milk Yield And Composition, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

EVERY DAIRY farmer knows how much he spends on the treatment of cows affected with mastitis, but the actual loss in production is more difficult to estimate.

As part of the current mastitis research programme the Department of Agriculture's Animal and Dairying Divisions surveyed 37 dairy herds with a believed high incidence of mastitis.