Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 4 of 4
Full-Text Articles in Medicine and Health Sciences
Appraisal Of Atp1b1 And Gstm3 Proteins As Freezability Factors In Buffalo Ejaculated Spermatozoa, Marvin Bryan Segundo Salinas, Thuchadaporn Chaikhun-Marcou, Phongsakorn Chuammitri, Anucha Sathanawongs
Appraisal Of Atp1b1 And Gstm3 Proteins As Freezability Factors In Buffalo Ejaculated Spermatozoa, Marvin Bryan Segundo Salinas, Thuchadaporn Chaikhun-Marcou, Phongsakorn Chuammitri, Anucha Sathanawongs
The Thai Journal of Veterinary Medicine
Sperm cryopreservation intensifies the use of assisted reproductive technologies in food animals. However, variations in freezability confront frozen-thawed semen applications. Hence, this groundwork aimed to identify the fundamental differences between high (HF) and low freezability (LF) ejaculated spermatozoa in swamp buffaloes, which were limitedly described for this agricultural species. Twelve (12) different samples of bubaline semen were collected, cryopreserved and categorized into freezability phenotypes using post-thaw sperm motility as the basis. Sperm kinematics and functional plasma membrane integrity were evaluated in HF and LF samples using computer-assisted sperm analysis (CASA) and hypo-osmotic swelling test, respectively. The relative concentrations of some …
Supplementation Of Extracellular Adenosine 5'-Triphosphate Improves Sperm Post-Thawed Quality In Thai Native Chickens, Phakjira Sanguansook, Junpen Suwimonteerabutr, Kaywalee Chatdarong
Supplementation Of Extracellular Adenosine 5'-Triphosphate Improves Sperm Post-Thawed Quality In Thai Native Chickens, Phakjira Sanguansook, Junpen Suwimonteerabutr, Kaywalee Chatdarong
The Thai Journal of Veterinary Medicine
Thai native chicken meat is a high-quality protein source of unique flavor. Genetic preservation and breeding programs are beneficial to maintain purebred populations. Aiming at improving post-thawed sperm quality, this study is the first to apply extracellular adenosine 5'-triphosphate (ATPe) in freezing extender to preserve Thai native chicken sperm. Eight semen samples from eight Thai native chickens (n = 8) were included. Initial semen evaluation and sperm size measurement were performed. Beltsville poultry semen extender supplemented with 0 and 30 mM ATPe was served as a control and treatment group, respectively. After thawing, motility and motion characteristics were evaluated by …
Cryopreservation Of Nili-Ravi Buffalo Bull Semen: Yearly Postthaw Analyses To Depict Climatic Effects, Ejaz Ahmad, Muhammad Shakeel, Zahid Naseer, Adeel Ahmed, Mudussar Nawaz, Muhammad Saleem Akhtar
Cryopreservation Of Nili-Ravi Buffalo Bull Semen: Yearly Postthaw Analyses To Depict Climatic Effects, Ejaz Ahmad, Muhammad Shakeel, Zahid Naseer, Adeel Ahmed, Mudussar Nawaz, Muhammad Saleem Akhtar
Turkish Journal of Veterinary & Animal Sciences
The present study was aimed to observe the postthaw quality of Nili-Ravi buffalo bull semen cryopreserved during heat stress (hot summer months) and normal climatic conditions (fall, winter, and spring months). For this purpose, four (n = 4) adult regular donor Nili-Ravi buffalo bulls were selected to collect and cryopreserve their semen throughout the period of an entire year. The cryopreservation of semen was done following standard freezing protocol. The semen doses cryopreserved during heat stress and normal climatic condition were thawed to determine the sperm motility, motion kinetics, viability, plasma membrane integrity, acrosomal integrity, and morphological abnormalities (head, mid-piece, …
Cryoprotective Effect Of Vaccenic Acid Supplemented In Bull Semen Extender, Fati̇h Avdatek, Umut Taşdemi̇r, Muhammed Enes İnanç, Deni̇z Yeni̇, Mehmet Fuat Gülhan, Şükrü Güngör, Ruhi̇ Türkmen, Pürhan Barbaros Tuncer
Cryoprotective Effect Of Vaccenic Acid Supplemented In Bull Semen Extender, Fati̇h Avdatek, Umut Taşdemi̇r, Muhammed Enes İnanç, Deni̇z Yeni̇, Mehmet Fuat Gülhan, Şükrü Güngör, Ruhi̇ Türkmen, Pürhan Barbaros Tuncer
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to investigate whether vaccenic acid (VA) added to TRIS semen extender has an effect on freezing bull semen. The semen was collected at intervals of three days through an artificial vagina then pooled. The pooled ejaculates were divided into five components as the control (C; no supplement) and VA (25, 50, 100, and 200 μg / mL) groups then extended with the TRIS-based medium. The samples were left in a cold air cabinet for cooling process (4 °C), then they were equilibrated for 4 h. Afterward, the samples in the groups were frozen by …