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Full-Text Articles in Medicine and Health Sciences
First Identification Of Neospora Caninum In Thailand, Than Kyaw, Prachin Virakul, Manop Muangyai, Wijit Banlunara
First Identification Of Neospora Caninum In Thailand, Than Kyaw, Prachin Virakul, Manop Muangyai, Wijit Banlunara
The Thai Journal of Veterinary Medicine
An immunohistochemical examination of aborted bovine tissues, collected from two dairy farms that had a high abortion rate (7%), detected the organism Neospora caninum (NC). Five out of 12 aborting cows (41.7%) were seropositive to NC using a competitive ELISA test. Neospora-tachyzoites were detected in the placenta of a seropositive aborting cow. Neither NC parasites nor cysts were detected in other fetal tissues. The identification of the Neospora-parasite and a high percentage of Neospora seropositive aborting cows, suggested NC as the possible cause of abortion. This is the first identification of the NC parasite in Thailand.
Immunoperoxidase Studies On Leptospirosis In Bovine Abortion, Ahmet Temur, Yavuz Seli̇m Sağlam
Immunoperoxidase Studies On Leptospirosis In Bovine Abortion, Ahmet Temur, Yavuz Seli̇m Sağlam
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to determine leptospirosis from aborted bovine fetuses. Tissue sections of the liver, lung, spleen and kidney were stained with avidin-biotin peroxidase complex, and antigen localization was examined. These examinations showed positive staining in eight (24.24%) out of 33 fetuses for the presence of leptospira antigens. In these eight cases, leptospira antigens were found in three lung (37.5%), two liver (25%), four kidney (50%) and four spleen (50%) samples. Microscopic studies demonstrated that leptospiral antigens were located in the interalveolar and interlobular septum of the lung; in the cytoplasm of hepatocytes and macrophages in the portal regions …
A Comparison Of The Effect Of Methyl-B-Cyclodextrin On The Osmotic Fragility Of Ovine, Bovine And Human Erythrocytes, Şevket Arikan
A Comparison Of The Effect Of Methyl-B-Cyclodextrin On The Osmotic Fragility Of Ovine, Bovine And Human Erythrocytes, Şevket Arikan
Turkish Journal of Veterinary & Animal Sciences
Cyclodextrins are used extensively in food, cosmetic, drug and chemical industries to increase aqueous solubility and stability, and reduce or eliminate the unpleasant taste and smell of many products. Experiments were conducted to examine the effect of a pre-haemolytic dose of methyl b-cyclodextrin (MBCD) on the osmotic fragility of erythrocytes collected from sheep, cattle and humans. Heparinised blood collected from these three species were analysed within 3 h of collection. Erythrocyte suspensions were mixed with MBCD to give concentrations of 2 mM, 4 mM or 6 mM cyclodextrin. The mixtures were incubated for 30 min at 37 °C and centrifuged. …
The Presence, Isolation And Characterisation Of Galectin-1 In Bovine Adrenal Gland, Kami̇l Seyrek
The Presence, Isolation And Characterisation Of Galectin-1 In Bovine Adrenal Gland, Kami̇l Seyrek
Turkish Journal of Veterinary & Animal Sciences
The mammalian adrenal gland in the developmental stage provides excellent system for studying the role(s) of lectins in the foetal period because the adrenal gland is composed of two separate endocrine organs which differ in embryologic origin, type of secretion, and function. There is no information on the presence of galectin-1 or galectin-1 reactive glycoconjugates in the development of the bovine adrenal gland. Therefore, the present study focused on this protein. For separation of galectin-1 dialysis, affinity chromatography and SDS-PAGE were used. To monitor the localisation of galectin-1 and its epitopes immunhistochemistry was used. A single 14-kD band comigrated with …
Transgenic Bovine Nuclear Transfer Embryos From Adult Somatic Cell Lines*, Sezen Arat, S. Jacek Rzucidlo, Steven L. Stice
Transgenic Bovine Nuclear Transfer Embryos From Adult Somatic Cell Lines*, Sezen Arat, S. Jacek Rzucidlo, Steven L. Stice
Turkish Journal of Veterinary & Animal Sciences
This study was performed to examine whether adult fibroblast cells can be manipulated genetically and whether clonal lines derived from those cells can support embryonic development. A primary adult cell line was established from the ear of an aged cow. Adult cells with a plasmid containing the enhancer green fluorescence protein (EGFP) gene, and non-transfected cells were used for cloning. Green fluorescence expression was observed in 35/49 (71.4%) adult clones. The developmental rates of embryos were significantly lower for cell lines expressing EGFP (11.4%) than for non-transfected cells (20.1%, P < 0.05). However, there was no decrease in nuclear transfer (NT) developmental rates (21.5%) when donor nuclei from EGFP transfected cell lines not expressing EGFP but retaining neomycin-resistance gene expression were used as donor nuclei. The NT embryos from transfected and non-transfected cell lines had similar morphology and cell numbers. The results indicated that adult cells can complete clonal propagation including transfection and selection and can be used to produce transgenic NT embryos.