Medicine and Health Sciences Commons^™

Strong Side, Weak Side: Goal Generating Tactics In Ncaa Men's Water Polo, Joey Gullikson

University of the Pacific Theses and Dissertations

In the game of water polo, it is generally accepted that the shooting position of the offensive player and the tactic employed are both important in generating goals. Despite their importance, little is known about the relationship between shooting position and offensive tactics and their impact on the probability of goal scoring. In this research, a sequence of hierarchical mixed logistic regression models is applied to a unique data set from 2016 and 2017 NCAA men’s water polo seasons to analyze the relationship between goal generating tactics and different shooting positions. The primary result reveals that the closer a player …

The Influence Of Fatiguing Exercise On Power Output, Lena Kate Perry

University of the Pacific Theses and Dissertations

Physical fatigue impairs performance during high power, short duration activities. As technological developments permit new methods of measuring this effect, it is important to validate existing paradigms. The purpose was to determine if kinetic measurements from vertical jump (VJ) tests are influenced by fatigue based on explosive power outputs. A sample of athletes (9 men, 26 women) from a Division I NCAA sports program completed testing. To establish baseline VJ kinetics, athletes performed a controlled warm-up and then completed six jumps using Sparta Science technology, each separated by 15s rest. Sparta software computed three force outputs: Load, Explode and Drive. …

A Cost Control Model For Inpatient Medications Among Adults With Mental And Behavioral Health Disorders, Huanan Li

University of the Pacific Theses and Dissertations

Pharmaceutical expenditures are an important part of the entire hospital operating budget, and inpatient pharmaceuticals denote one of the highest costs in hospital care. Predictions for medication budgets based on the types of patients have been largely undertaken in medical hospitals and not psychiatric facilities. According to several previous studies, gender, age, diagnosis, comorbidity and length of stay (LOS) affect the general inpatient treatment expenditures. However, whether or not the impact of these factors differs in psychiatric hospitals remains to be investigated. To that end, the current study examines medication costs for mental and behavioral health disorder as well as …

Synthesis And Conformational Studies Of Various Amides, Marcos Beltran-Sanchez

University of the Pacific Theses and Dissertations

In the past, aminocyclohexanol rings have been successfully utilized as pH-triggered molecular switches in various trans-2-aminocyclohexanol derivatives. By changing the groups on the amine nitrogen, these models provided a wide pH range in which a switch can occur. The pH-induced switch of conformation was monitored by 1H-NMR spectroscopy. The models were also incorporated into the bilayer membrane of liposome structures and tested for their ability to disrupt their membrane upon their conformational flip induced by a decrease in pH.

In this work, the amide bond has been studied as a molecular switch and various amide derivatives have been tested for …

Predictors Of Morbidity And Mortality Among Thoracic Trauma Patients, Jeremy Patrick Mcconnell

University of the Pacific Theses and Dissertations

Background. There are roughly 300,000 rib fractures treated for in the United States each year. These represent 10-26% of thoracic trauma injuries and have about a 10% mortality rate. There is a common belief that mortality in rib fracture patients can be contributed to the diagnosis of pneumonia, but this study does not support that claim. Purpose. To determine the predictors of morbidity and mortality in rib fracture patients. Methods. Using a level 1 trauma center patient registry, we retrospectively analyzed all patients that were admitted with at least one rib fracture (n=1,344). All predictors were analyzed with linear regressions. …

The Role Of Aryl Hydrocarbon Receptor (Ahr) In Drug-Drug Interaction And The Expression Of Ahr In Pichia Pastoris, Yujuan Zheng

University of the Pacific Theses and Dissertations

The aryl hydrocarbon receptor is a ligand-activated transcription factor that is involved in many important functions in the body. To study the role and function of AHR, an abundant amount of in vitro expressed and purified protein is needed. A baculovirus insect expression system is commonly employed to express AHR, however, there are several drawbacks with this method, such as mutation potential and high cost. A better in overexpression system is needed and we hypothesize that Pichia pastoris, a yeast expression system, could stably express AHR and ARNT (aryl hydrocarbon receptor nuclear translocator) in sufficient amount with reasonable cost. Codon …

Goal Orientations And Beliefs About Success In Age Group Swimmers, Nathan Alexander Rhea

University of the Pacific Theses and Dissertations

The purpose of this study was to expand on goal orientation theory and its relationship with beliefs about the causes of sport success by differentiating by age group in youth USA Swimming registered swimmers, ages 11-18. 80 swimmers from six different USA Swimming clubs completed the Task and Ego Orientation in Sport Questionnaire (TEOSQ) and the Beliefs About the Causes of Sport Success Questionnaire (BACSSQ) after providing parent consent and child assent to take part in the study. Similar to previous research, the ego orientation was found to have a significant positive relationship with ability and deception as beliefs about …

Glycemic Control In A Type I Diabetic Athlete: Recommendations For Athletic Trainers In Management, Athlete Care, And Performance, Megan Whyte

University of the Pacific Theses and Dissertations

Type I diabetes in athletes is a relatively rare condition and as a certified athletic trainer it is critical to know how to manage all aspects of this disease in an athlete. Current National Athletic Trainers’ Association (NATA) guidelines exist for developing a basic plan for management and care of an athlete with Type I Diabetes, but there is room to improve current guidelines, using evidence-based practice. Purpose: to address: medical management of a type I diabetic athlete as a case study, obtain first person observations from the athlete’s perspective, and provide additional evidence based practice recommendations to athletic trainers …

The Potency Of Ag10 In Stabilizing Transthyretin Is Driven By Interactions Mimicking The Disease-Suppressing T119m Variant, Dengpan Liang

University of the Pacific Theses and Dissertations

Transthyretin (TTR) amyloid cardiomyopathy (ATTR-CM) is a fatal disease with no available disease-modifying therapies. While destabilizing TTR mutations increase the risk of developing ATTR-CM, the naturally occurring Thr119Met (T119M) variant stabilizes TTR and prevent disease. The two Serine 117 and Serine 117’ (S117/S117’) side chain hydroxyl groups of monomers A and B in T119M variant TTR form direct hydrogen bonds with each other. AG10 is an orally available, small molecule TTR stabilizer in Phase 3 clinical development for ATTR-CM. Structural features that infer high potency of AG10 for stabilizing TTR remains incompletely characterized.

In order to investigate the contribution of …

Exercise And Patient Populations: A Community-Based Exercise Intervention On Cancer And Type 2 Diabetes, Cynthia Villalobos

University of the Pacific Theses and Dissertations

Cancer and diabetes are the second and seventh leading causes of death in the United States, respectively (Kochanek et al., 2017). When including prediabetes and the entire cancer continuum, both diseases affect over 150 million individuals in the United States each year (Bullard et al., 2018; Siegel et al., 2019). Furthermore, there exists an alarming rise in patients presenting with both type 2 diabetes and obesity-related cancers concurrently, as both diseases share similar risk factors (Vigneri et al., 2009). An aging adult population, physical inactivity and unhealthy eating habits are continuing to rise, and the prevalence of individuals with obesity-related …

Lipid-Coated Magnesium Phosphate Nanoparticles For Intrapulmonary Protein Delivery In Mice, Mallika Vadlamudi

University of the Pacific Theses and Dissertations

Proteins are a diverse category of biomolecules with great therapeutic potential. Intracellular delivery of proteins can augment the deficient activities of dysfunctional or poorly expressed innate proteins and therefore represents a promising strategy to treat the associated diseases. One major barrier to intracellular protein delivery is the translocation of the protein across the cellular membrane. Endocytosis provides an important pathway for protein nanocarriers to enter cells across the plasma membrane. However, the cargo protein must then promptly escape from the endosomes to avoid degradation in the lysosome and to exert its cellular function.

Previously, we reported a cationic lipid-coated magnesium …

Oral Health Care: An Autoethnography Reflecting On Dentistry's Collective Neglect And Changes In Professional Education Resulting In The Dental Hygienist Being The Prevention-Focused Primary Oral Health Care Provider, Maureen Patricia Mcweeney Harrington

University of the Pacific Theses and Dissertations

Many factors influence poor oral health among disadvantaged populations including socioeconomic circumstances, knowledge of disease prevention strategies and ability to implement those strategies, public policies, insurance status, insurance policies, dental providers and other challenges to accessing dental care. Often these issues converge and result in early disadvantages to achieving good oral health (Horton & Barker, 2010). Addressing even some of the factors that contribute to poor oral health may provide ways to change the dental health status of historically underserved populations. The purpose of this research is to explore my role as a practitioner and researcher in the creation of …

Development Of A New Class Of Ligand-Targeted Chemotherapeutics With Enhanced Safety And Efficacy Profile, Arindom Pal

University of the Pacific Theses and Dissertations

The hydrophobicity of many chemotherapeutic agents usually results in their nonselective passive distribution into healthy cells and organs causing collateral toxicity. Ligand-targeted drugs (LTDs) are a promising class of targeted anticancer agents. The hydrophilicity of the targeting ligands in LTDs limits its nonselective passive tissue distribution and toxicity to healthy cells. In addition, the small size of LTDs allows for better tumor penetration, especially in the case of solid tumors. However, the short circulation half-life of LTDs, due to their hydrophilicity and small size, remains a significant challenge for achieving their full therapeutic potential. Therefore, extending the circulation half-life of …

I3: Isomerization Of Isomer Ions, Krisztián Gabor Torma

University of the Pacific Theses and Dissertations

Photoelectron Photoion Coincidence (PEPICO) spectroscopy is a robust tool for elucidating complex unimolecular dissociation mechanisms and for determining thermochemical and kinetic data of gas-phase ion dissociations with high accuracy. In this work, the dissociative photoionization of two sets of isomeric systems were analyzed with PEPICO: 1) C7H7+ ions of toluene (Tol) and 1,3,5-cycloheptatriene (CHT), and 2) two butyl alcohol isomers, 1-butanol and isobutanol. Threshold dissociative photoionization data on these four molecules of interest were collected on the imaging PEPICO apparatus at the VUV beamline of the Swiss Light Source. Data analysis was aided by ab initio calculations and Rice-Ramsperger-Kassel-Marcus (RRKM) …

Developing A Rational Peptide Ligand Design Method Using Cd13 As A Prototype Target Receptor, Md Zahir Uddin

University of the Pacific Theses and Dissertations

Structure based computational peptide design methods have gained significant interest in recent years with the availability of structural insights of protein-protein interactions obtained from the crystal structures. Most of these approaches design new peptide ligands by connecting the crucial amino acid residues from the protein interface and are generally not based on any predicted receptor-ligand interaction. In this work, a peptide design method based on the Knob-Socket model was used to identify the specific ligand residues packing into the receptor interface. This method enables rational peptide ligand design by predicting amino acid residues that will fit best at the binding …

Effects Of A Group-Deposit Prize Draw On The Step Counts Of Adults, Alex J. Mccurdy

University of the Pacific Theses and Dissertations

The World Health Organization (WHO, 2016) reports that 3.2 million deaths per year are attributable to physical inactivity, making it the fourth leading risk factor for global mortality. Physical inactivity is also a key risk factor for noncommunicable diseases such as cardiovascular disease, cancer, and diabetes (WHO, 2018). Globally, 1 in 4 adults is not active enough and, therefore, foregoes a myriad of health benefits associated with Physical Activity (PA; WHO, 2018). In the United States, only about 1 in 5 (21%) adults meet the 2008 Physical Activity Guidelines set by the Centers for Disease Control and Prevention (CDC, 2018). …

Fliposomes With A Ph-Sensitive Conformational Switch For Anticancer Drug Delivery Against Triple Negative Breast Cancer, Yifan Lu

University of the Pacific Theses and Dissertations

Cancer is the second leading cause of death in the US and worldwide, accounting for 16% of deaths worldwide in 2015. Of more than 100 types of cancers affecting humans, breast cancer is the most common cancer among women and is the second leading cause of death in women. Triple negative breast cancer (TNBC) is a subtype of breast carcinomas defined by the lack of the expression of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor (HER2 /neu). The prognosis and survival of TNBC patients remains the poor due to the lack of effective targeted therapy. …

Intrapulmonary Inoculation Of Multicellular Tumor Spheroids To Construct An Orthotopic Lung Cancer Xenograft Model That Mimics Four Clinical Stages Of Non-Small Cell Lung Cancer, Yingbo Huang

University of the Pacific Theses and Dissertations

Lung cancer leads in mortality among all types of cancer in the US and Non-small cell lung cancer (NSCLC) is the major type of lung cancer. Immuno-compromised mice bearing xenografts of human lung cancer cells represent the most common animal models for studying lung cancer biology and for evaluating potential anticancer agents. However, orthotopic lung cancer models based on intrapulmonary injection of suspended cancer cells feature premature leakage of the cancer cells to both sides of the lung within five days, which generates a quick artifact of metastasis and thus belies the development and progression of lung cancer as seen …

The Role Of Sex Hormones On Monocarboxylate Transporter Expression In Tissues Related To Drug Disposition, Jieyun Cao

University of the Pacific Theses and Dissertations

Proton- and sodium-dependent monocarboxylate transporters (MCTs (SLC16A) and SMCTs (SLC5A)) transport monocarboxylates such as ketone bodies, lactate and pyruvate, as well as drugs such as gamma-hydroxybutyric acid. CD147 acts as an ancillary protein for MCT1 and MCT4, and is involved in membrane trafficking. Previously, it has been shown that MCT expression changes under different sex hormone conditions in skeletal muscle and Sertoli cells. However, it is unknown if MCTs, SMCTs or CD147 demonstrate sex differences in tissues where they play an important role in drug disposition. Monocarboxylate transporter substrates GHB and valproic acid have demonstrated sex differences in pharmacokinetic profiles. …

Sexual Dimorphism In Aortic Function Of Uc Davis Type 2 Diabetes Mellitus Rat Model: Estrogen Specific Responses, Farjana Akther

University of the Pacific Theses and Dissertations

Little is known about the interaction between diabetes and sex in vasculature. This study was designed to investigate the effects of estrogen as well as type 2 diabetes (T2D) on aortic function in rats with respect to sex. To test the effects of T2D and sex, UC Davis Type 2 Diabetes Mellitus (UCD-T2DM) rat model was used. To study the effects of estrogen, ovariectomized Sprague- Dawley female rats and UCD-T2DM rats at pre-diabetic stage were used and the rats were implanted subcutaneously either with placebo or 17 β-estradiol pellets (60 days release, 1.5mg/pellets). The plasma analytes for metabolic parameters and …

Development And Characterization Of Ldv Peptide Targeted Nanocarriers For Paclitaxel Delivery: A Comparative Study Of Micelles, Liposomes And Solid Lipid Nanoparticles, Poonam Dattani

University of the Pacific Theses and Dissertations

Nanocarriers have been established as delivery vehicles to target cancer tumors. However, premature drug leakage is one of the major reasons for inefficient drug delivery of nanocarriers to the tumor. Drug diffusion out of the nanocarriers or destabilization of drug loaded nanocarriers by physiological interactions with blood cells, serum proteins, and cell membranes upon systemic administration contribute to premature drug release. In this study, targeted micelles, liposomes and solid lipid nanoparticles (SLNs) of similar composition were prepared and characterized to compare physicochemical characteristics, in vitro stability, in vitro release rates in release media and in vivo performance. Peptide Amphiphiles (PAs) …

The Effects Of Mindfulness Practice With Music Listening On Working Memory, Emily Irene Messick

University of the Pacific Theses and Dissertations

The purpose of this experimental study was to investigate mindfulness strategies and their influence on working memory. The potential role of music in facilitating mindfulness practice is explored. Various listening exercises were investigated along with their influence on working memory (i.e. attention control). Thirty-four individuals were randomly assigned to participate in one of four listening groups: 1) mindfulness with music, 2) mindfulness without music, 3) music only, and 4) silence. Thirty-four participants engaged in a computerized digit-span task before and after the listening exercise to assess pre- and post-test working memory performance. Thirty participants were included in data-analysis due to …

The Effect Of Energy Drinks On Cardiovascular Variables: A Randomized Controlled Trial, Cynthia Lee

University of the Pacific Theses and Dissertations

Many studies have investigated the effects of energy drinks on cardiovascular parameters. These studies were typically conducted with high volume (32 ounces) energy drinks and have demonstrated association with QT prolongation and blood pressure elevation after consumption. Currently, there is inconclusive evidence with lower volume energy drinks. This study intends to evaluate the effects of the largest commercially available energy drink can (24 ounces) in the United States on cardiovascular parameters compared with placebo arm. A randomized, double-blinded, placebo-controlled, crossover trial was conducted over 2 separate days with a minimum of 6-day wash out period. Healthy volunteers between the age …

Knob-Socket Investigation Of Stability And Specificity In Alpha-Helical Secondary And Quaternary Packing Structure, Taylor Renee Rabara

University of the Pacific Theses and Dissertations

The novel knob-socket (KS) model provides a construct to interpret and analyze the direct contributions of amino acid residues to the stability in α-helical protein structures. Based on residue preferences derived from a set of protein structures, the KS construct characterizes intra- and inter-helical packing into regular patterns of simple motifs. The KS model was used in the de novo design of an α-helical homodimer, KSα1.1. Using site-directed mutagenesis, KSα1.1 point mutants were designed to selectively increase and decrease stability by relating KS propensities with changes to α-helical structure. This study suggests that the sockets from the KS Model can …

Saudi Arabian Students In Postgraduate Dental Programs: Investigating Factors Associated With Burnout, Asiri Amal

University of the Pacific Theses and Dissertations

Burnout related to emotional and physical study or work demands affects an individual’s performance and well being. This study focused on Saudi Arabian dental residents studying in the United States and the United Kingdom who are faced with many challenges in pursuit of a higher education degree. A survey including demographic and MBI questions was distributed to assess this population’s level of burnout. The Maslach Burnout inventory (MBI) was has been widely used in the literature to assess three components of burnout: emotional exhaustion (EE), depersonalization (DEP), and (diminished) personal accomplishment (PA). Potential predictors of burnout level, tested for statistical …

Investigating The Effect Of Rutaecarpine On The Benzo[A]Pyrene-Induced Dna Damage In Vitro, You Li

University of the Pacific Theses and Dissertations

Benzo[a]pyrene (BaP), is one of the most potent mutagens and carcinogens known. It requires metabolic activation through cytochrome P450 (CYP)1A1 to yield the ultimate carcinogenic metabolite, benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE). BPDE can bind to DNA and form predominantly covalent (+) trans adducts at the N2 position of guanine causing DNA damage. Rutaecarpine (RTC) is an herbal medicine that has been used to treat several diseases such as headache, hypertension, gastrointestinal disorders, amenorrhea, and anti-inflammation. It has also been reported as a potent inducer of CYP enzymes, including CYP1A1, and CYP1A2. The mechanisms underlying up-regulation of CYP1A1 by RTC is dependent on aryl …

Visual Feedback In Voice Therapy For Individuals With Parkinson's Disease, Rachel Brooke Convey

University of the Pacific Theses and Dissertations

Parkinson ’s disease (PD) is a progressive neurological disorder that affects one’s movement. As the disease develops, individuals begin to present with symptoms that include but are not limited to bradykinesia, rigidity, tremors, and hypokinetic dysarthria. These symptoms affect a person’s entire body, including his/her voice. The Lee Silverman Voice Treatment (LSVT) program for treating individuals with PD is supported by over twenty-five years of research. It is considered a safe, non-invasive method to improve vocal loudness and speech clarity in individuals with PD. However, simply because LSVT is effective in its current state, it does not mean that the …