Medicine and Health Sciences Commons^™

Cognition In Health And Disease: Two Mouse Model Studies, Heather Lynn Mahoney

USF Tampa Graduate Theses and Dissertations

Cognitive and circadian dysfunction are common aspects of neuropsychiatric disease that are often overlooked in disease models and treatment strategies. In this work, we first evaluated the potential for cognitive enhancement in healthy adult mice by targeting a kinase involved in regulation of the molecular circadian clock. We then developed and characterized a model of neuropsychiatric disease that could present a target for rescuing circadian and cognitive deficits using this treatment.

Time-of-day effects have been noted in a wide variety of cognitive behavioral tests, and perturbation of circadian rhythms impairs hippocampus-dependent learning and memory. We first asked whether casein kinase …

Quantifying The Impact Of Chronic Stress On Racial Disparities In Cardiovascular Disease, Nnadozie Emechebe

USF Tampa Graduate Theses and Dissertations

Background: Despite declining mortality in cardiovascular diseases (CVD), racial disparities between non-Hispanic Blacks (NHB) and to non-Hispanic Whites (NHW) persist. Although the prevalence of traditional risk factors of CVD such as hypertension, is higher in NHB compared to NHW, adjusting for this difference does not eliminate the disparity completely. This suggests other factors might explain the persisting disparities. Thus, the purpose of this dissertation is to quantify the impact of chronic stress in explaining the racial disparities in cardiovascular diseases (CVD). This dissertation contains three studies that addressed the following Specific Aims: Specific aims: 1) To create and assess the …

Hearing Aid Acclimatization In The Context Of Central Gain Mechanisms, Peter J. Hutchison

USF Tampa Graduate Theses and Dissertations

The primary goals of this dissertation were to 1) identify markers of central gain in the auditory system following short-term acoustic attenuation via earplugging and acoustic enhancement via sound generators, and 2) determine if acoustic enhancement is effective in modulating central gain in older adults with age-related hearing loss, and if so, to what extent. Additionally, a further goal of this dissertation was to explore the possibility that central gain is related hearing aid acclimatization. The results described in Chapter 2 are further evidence that altering acoustic input to the peripheral auditory system modulates central auditory plasticity and is evident …

Spatial And Temporal Determinants Associated With Eastern Equine Encephalitis Virus Activity In Florida, Kristi M. Miley

USF Tampa Graduate Theses and Dissertations

Eastern Equine Encephalitis virus (EEEV) is considered the most pathogenic mosquito-borne illness in the United States. Human mortality has been estimated to range between 35 to 75%, with horses experiencing mortality rates greater than 90%. A large number of EEEV cases occur in Florida. Though we have come a long way since the first human infection was identified in the 1930s, there is still much to learn regarding the virus’s ability to maintain transmission year-round in Florida. Phylogenic studies support that Florida may serve as the geographic reservoir for EEEV. This research investigated spatiotemporal and ecological variables associated with risk …

Evaluation Of Aging And Genetic Mutation Variants On Tauopathy, Amber M. Tetlow

USF Tampa Graduate Theses and Dissertations

Alzheimer’s disease (AD) is characterized by amyloid β plaques and neurofibrillary tau tangles (NFTs). While research has demonstrated amyloid pathology occurs prior to tau pathology, or tauopathy, tau has proven to be more toxic. Tauopathy is associated with cognitive declines and neurodegeneration. These findings have highlighted the importance of further understanding tauopathy. In the progression of tauopathy, there is an observable immune response that can be measured by glial cells such as microglia. Activated microglia are known to exacerbate tauopathy rather than reducing the pathology. Research has indicated that with increased age there is an increased risk for AD-related tauopathy …

The Effect Of Hope On The Relationship Between Personal And Disease Characteristics And Anxiety And Depression In Adolescents And Young Adults With Cancer, Sharon B. Mcneil

USF Tampa Graduate Theses and Dissertations

Adolescents and young adults (AYAs) with cancer face physical, psychosocial, emotional, and developmental challenges that are unique to their age group. In facing the crisis of a cancer diagnosis, both social support and hope have been shown to be tools utilized by some AYAs. However, AYAs have higher rates of anxiety and depression than in older adults which may have an effect on their levels of hope.

This cross-sectional study examined the mechanistic effects of hope on the relationship between personal characteristics of age, gender, and social support, the disease characteristics of type of cancer and length of time since …

Immune Modulation As A Therapeutic Target In An Α-Synuclein Model Of Parkinson’S Disease, Meena Subhashini Subbarayan

USF Tampa Graduate Theses and Dissertations

Parkinson’s disease (PD) is the second most common neurodegenerative disorder affecting about 1.5 million people in the United States with more than 60,000 people diagnosed each year. It is classically characterized by four major symptoms: tremor, postural instability, stiffness in joints, and slow movement (bradykinesia). Pathologically PD is characterized by up to 70% loss of dopaminergic neurons in substantia nigra pars compacta (SNpc) of midbrain and accumulation of presynaptic protein called α-synuclein (α-syn) within dopaminergic neurons that extend to the striatum. This disrupts the nigrostriatal pathway leading to the motor symptoms seen in PD patients. Microglia, the innate immune cells …

Longitudinal Examination Of A Dual-Factor Model Of Mental Health: Academic Adjustment And Stability Of Group Membership In High School Students In Academically Accelerated Curricula, Letty Langton Dileo

USF Tampa Graduate Theses and Dissertations

Middle adolescence (ages 14 to 18 years old) has been associated with declines in both psychopathology and subjective well-being (SWB). This study examined a dual-factor model (DFM) of mental health, which conceptualizes complete mental health as including both low levels of psychopathology and high levels of SWB, across three time points, each 9-12 months apart, in a sample of 328 9th grade students enrolled in accelerated coursework. This study aimed to determine (1) the stability of students’ mental health status over time, (2) the role of psychopathology versus SWB for students who changed mental health status, and (3) the relationship …

A Review Of American College Campus Tobacco Or Smoke Free Policies: A Case Study Of A Large Urban University, Sarah E. Powell

USF Tampa Graduate Theses and Dissertations

Objective: A year after a tobacco free policy was passed, the University of South Florida (USF) was interested in the overall policy opinions and efficacy. To assess this a project was completed that included a survey and geospatial data. The survey measured tobacco policy enforcement behavior and a geographic information system (GIS) mapped tobacco policy violation. This project introduces an evaluation process that can efficiently assess an institutions tobacco-free policy. Methods: Using a cross-sectional survey sent to students, faculty, and staff, understanding of policy and resources, tobacco use observations, stage of change regarding policy enforcement, self-efficacy to enforce, and perceived …

Physician Self-Efficacy And Risk-Taking Attitudes As Determinants Of Upcoding And Downcoding Errors: An Empirical Investigation, Samantha J. Champagnie

USF Tampa Graduate Theses and Dissertations

Physicians across the United States are burdened with the pressure of accurate coding while trying to maintain quality patient care. Despite the economic importance of coding accuracy, investigators have not reached consensus on the factors that contribute to coding errors. This study fills this gap by investigating physician characteristics that explain the variation in physician coding accuracy, specifically evaluation and management upcoding and downcoding errors. An electronic survey was distributed to 325 physicians that measured physicians’ attitudes towards risk and coding self-efficacy. Regression analysis found physicians with low self-efficacy had more conservative coding behaviors, resulting in higher incidences of downcoding. …

On The Importance Of Context: Examining The Applicability Of Infertility Insurance Mandates In The United States Using A Mixed-Methods Study Design, Nathanael B. Stanley

USF Tampa Graduate Theses and Dissertations

Accessibility of infertility services is disproportionately experienced in the United States. Although there exist state-based health insurance mandates for infertility services, these mandates contain language that disqualify people from using them. In order to better understand why these mandates are not able to reduce the financial burden and bridge the income disparity for using infertility services, the purpose of this study is to add context to the applicability of these insurance mandates through qualitative and quantitative inquiry. Using the Glass and McAtee model of risk regulators as an operational paradigm, this research explores the role of environmental context, or “place”, …

Functional Gait Asymmetries Achieved Through Modeling And Understanding The Interaction Of Multiple Gait Modulations, Fatemeh Rasouli

USF Tampa Graduate Theses and Dissertations

Walking is an important determinant of human functionality. Gait disabilities affect millions of people worldwide every year. Investigating the science of walking advances recovery techniques and assistive devices for gait rehabilitation. A functional gait promotes productivity, independence, and quality of life. Human gait, like any other moving mechanism, is a dynamic system. Understanding and analyzing the dynamic aspects of gait improves the recovery methods to fundamentally affect and interact with lower limbs.

This dissertation aims to fill the gaps in mechanical simulations of gait and dynamic analysis of rehabilitation techniques. The solutions consider kinematic, kinetic, and spatiotemporal parameters of gait …

Ssh1 Impedes P62/Sqstm1 Flux And Tau Clearance Independent Of Cofilin Activation, Cenxiao Fang

USF Tampa Graduate Theses and Dissertations

Accumulation of toxic protein assemblies and damaged mitochondria are key features of neurodegenerative diseases, which arise in large part from clearance defects in the autophagy-lysosome system. The autophagy cargo receptor p62/SQSTM1 plays a major role in the clearance of ubiquitinated cargo through Ser403 phosphorylation by multiple kinases. However, no phosphatase is known to physiologically dephosphorylate p62 on this activating residue. RNAi-mediated knockdown and overexpression experiments using genetically encoded fluorescent reporters and defined mutant constructs in cell lines, primary neurons, and brains show that SSH1, the canonical cofilin phosphatase, mediates the dephosphorylation of phospho-Ser403-p62, thereby impairing p62 flux and phospho-tau clearance. …

The Impact Of Myeloid-Mediated Co-Stimulation And Immunosuppression On The Anti-Tumor Efficacy Of Adoptive T Cell Therapy, Pasquale Patrick Innamarato

USF Tampa Graduate Theses and Dissertations

Adoptive T cell therapy (ACT) in combination with lymphodepleting chemotherapy is an effective strategy to induce the eradication of tumors, providing long-term regression in cancer patients. However, only a minority of patients that receive ACT with tumor infiltrating lymphocytes (TILs) exhibit durable benefit. Thus, there is an urgent need to define strategies that potentiate anti-tumor activity conducted by adoptively transferred T cells. In these studies, we aimed to identify novel strategies to enhance the therapeutic efficacy of ACT. Accordingly, we describe the disparate roles of myeloid cells in the context of ACT characterized by the augmentation of TIL proliferation in …

Effects Of The 340b Drug Pricing Program On Hospitals’ Prescribing Behavior, Patient Mix, And Quality Of Care, Yilu Dong

USF Tampa Graduate Theses and Dissertations

In 1992, Congress created the 340B Drug Pricing Program that requires drug manufacturers to provide outpatient drugs to participating hospitals with substantial discounts. Although the intent of the program is to allow covered entities to increase access to care for more vulnerable patients, hospitals are not required by law to pass on the discounts. Therefore, a concern is that hospitals might over-prescribe. This dissertation includes three chapters to study the effects of the 340B program on hospitals’ behavior changes:

Chapter 1 uses state aggregate hospital service spending data from the Centers for Medicare and Medicaid Services (CMS) to study the …

In Post-Extubated Patients What Are The Preferred Methods Of Communication During Their Experience Of Endotracheal Intubation With Mechanical Ventilation, Lanette Dumas

USF Tampa Graduate Theses and Dissertations

To date, communication between mechanically intubated patients and nurses is laden with negative psychological and physiological impacts. Research has focused more on what patients want to communicate, and how nurses communicate with intubated patients. There is limited research identifying the communication methods preferred by these patients. The purpose of this study was to identify communication preferences of mechanically intubated patients using a phenomenological approach.

Semi-structured interviews were conducted with participants in their hospital rooms. The interview questions focused on the period of mechanical intubation when the participants were unable to speak. A total of 27 participants were interviewed at a …

Temporal Discounting And The Assessment And Treatment Of Academic Procrastination, Anthony Concepcion

USF Tampa Graduate Theses and Dissertations

Many individuals engage in procrastination at some point in their lifetime. Although procrastination is usually not detrimental, for college students, academic procrastination is correlated with adverse health effects (e.g., anxiety, depression, sleep hygiene) and poor academic performance (Akinsola, et al., 2007; Ferrari, et al., 1995). Furthermore, the prevalence of academic procrastination is high with reports of up to 95% of college students engaging in detrimental amounts of procrastination (Hussain & Sultan, 2010). Notably, students enrolled in online courses are likely to be at greater risk to experience adverse consequences associated with procrastination (Elvers, et al, 2003). Previous studies have focused …

Evaluating Public Posting, Goal Setting, And Rewards To Increase Physical Activity In Children, Bryon G. Miller

USF Tampa Graduate Theses and Dissertations

In Experiments 1 and 2 we evaluated a pedometer-based intervention consisting of public posting between two teams of students, with additional self-monitoring, goal setting, and reinforcement components, to increase physical activity during school recess. In the absence of self-monitoring, performance feedback alone did not increase physical activity levels above those observed during baseline. Additionally, higher levels of physical activity were observed when goal-setting was introduced, with the highest levels of activity observed when raffle tickets could be earned for exceeding a specified step-total goal. In Experiment 3 we removed the team component and evaluated similar intervention components across an entire …

Assisted Living Facilities In Florida: Examining The Factors Associated With Entries, Closure, And Licensure Change, Joseph W. June

USF Tampa Graduate Theses and Dissertations

Assisted living facilities (ALFs) have become a large provider of long-term care in the United States. Their expansion has been met with interest from older adults who desire to be independent yet receive some services and from state legislators who recently have attempted to increase access. Studies in long-term care have focused extensively on nursing homes, but recent research has examined the effects of ALFs on the long-term care market. Research has been lacking in understanding the unique markets of ALFs. Describing themselves as “communities” and offering multiple options for housing, ALFs have targeted independent and wealthier older adults who …

Active Deep Learning Method To Automate Unbiased Stereology Cell Counting, Saeed Alahmari

USF Tampa Graduate Theses and Dissertations

Cell quantification in histopathology images plays a significant role in understanding and diagnosing diseases such as cancer and Alzheimers. The gold-standard for quantifying cells in tissue sections is the unbiased stereology approach. Unfortunately, in unbiased stereology current practices rely on a well-trained human to manually count hundreds of cells in microscopy images. However, this human-based manual approach is time-consuming, labor-intensive, subject to human errors, recognition bias, fatigue, variable training, poor reproducibility, and inter-observer error. Thus, the lack of high-throughput technology for automating unbiased stereology analyses remains a major obstacle to further progress in a wide range of neuroscience and cancer …

Indicators Of Mild Cognitive Impairment Associated With Language Processing And Production, Diana Julbe-Delgado

USF Tampa Graduate Theses and Dissertations

The research purpose of the present study was to (1) examine cognitive-linguistic features related to processing and production across a series of tasks that are representative of everyday discourse and (2) compare older adults with and without mild cognitive impairment (MCI) across linguistic features. Twenty-seven participants, including 12 individuals with- and 15 individuals without MCI, were enrolled from a larger study (Hudak et al., 2019). Cognitive status was initially assessed as part of the larger study using the Montreal Cognitive Assessment (MoCA; Nasreddine et al., 2005). Participants who scored ≤ 25 on the MoCA received a standardized neuropsychological evaluation and …

Mechanistic And Translational Studies On Skeletal Malignancies, Jeremy Mcguire

USF Tampa Graduate Theses and Dissertations

New treatment strategies are desperately needed for treating skeletal malignancy. Skeletal malignancies can be either primary cancer that originated in the bone, such as osteosarcoma, or metastatic cancer that spread from another organ to the skeleton, as in the case of breast or prostate cancer. In this thesis, I will detail two projects that focus on the discovery of new treatment strategies for both primary skeletal malignancy and metastatic skeletal malignancy.

The first project focuses on the primary skeletal malignancy, osteosarcoma, a rare cancer that is commonly diagnosed in children and young adults and metastasizes to the lungs. The survival …

Using Observations From The Uaw-Ford Ergonomic Assessment Tool To Predict Distal Upper Extremity Musculoskeletal Disorders, Zachariah T. Brandes-Powell

USF Tampa Graduate Theses and Dissertations

Distal upper extremity (DUE) musculoskeletal disorders (MSDs) affect thousands of workers annually. Job tasks can have a significant amount of MSD risk depending on the presence of certain risk factors The UAW-Ford Ergonomic Surveillance Tool (EST) is a method specifically designed for the UAW and Ford Motor Company to assess MSD risk in its facilities. The purpose of this report is to evaluate which risk factors in the UAW-Ford EST predict the risk of developing DUE MSDs. Data collected by a previous study on four UAW-Ford automotive manufacturing plants were analyzed. Target jobs were selected based on associated First Time …

Enhancing Intergenerational Conversation Using Visual Cues: Effects Of A Historical Timeline, Allyson Lindsay

USF Tampa Graduate Theses and Dissertations

Interacting with individuals with expressive aphasia can be particularly challenging not only because of the language impairments, but also because of the frequent age difference between the clients with aphasia and student clinicians. Although students learn a variety of strategies to support language impairments, there remains a need to bridge the age gap in historical knowledge to enhance conversation. The current study explored the use of a timeline as written and pictorial cue to support conversations between individuals with aphasia and students. This study consisted of Two Experiments. The purpose of Experiment 1 was to identify the differences in knowledge …

Evaluating Small-Scale Simulation For Teaching Abduction Prevention Skills, Jessica E. Ritzmann

USF Tampa Graduate Theses and Dissertations

Approximately 1,600 attempted kidnappings occurred within 2018 (National Center for Missing & Exploited Children, 2019). In a 2016 report by Wolak, Finkelhor, and Sedlak, of the 105 stereotypical kidnappings that occurred in 2011, many resulted in sexual assault, the children are never found, or death. These dangerous circumstances warrant a method of teaching safety skills to children to avoid abduction. Research has shown that small-scale simulation is effective for teaching firearm safety to 3- to 5-year-olds children (Maxfield, Miltenberger, & Novotny, 2019). This research has not been applied to abduction prevention safety. The current study aimed to address this research …

Hyaluronic Acid Coated Targeted Lipid Micellar Nanoparticle As A Delivery Vehicle For Lapatinib And Ketoconazole In Egfr Mutated Lung Cancer, Nadia Tasnim Ahmed

USF Tampa Graduate Theses and Dissertations

Background: According to the American Cancer Society, lung cancer was accountable for over 142,000 deaths in the USA in 2019. Common mutations related to lung cancer mainly occur in TP53, EGFR, and KRAS genes. Lapatinib is a small molecule tyrosine kinase inhibitor which acts reversibly on both epidermal growth factor receptor (EGFR) and human epidermal growth factor receptor 2 (HER2). Overexpression of EGFR leads to increased production and signaling of proteins that cause upregulation of cellular proliferation, cholesterol synthesis and resistance to apoptosis. Ketoconazole is an imidazole antifungal agent which works principally by inhibiting the enzyme cytochrome P450 14α-demethylase (CYP51A1) …

Using Observations From The Uaw-Ford Ergonomic Assessment Tool To Predict Low Back Musculoskeletal Disorders, Colins Nwafor

USF Tampa Graduate Theses and Dissertations

Musculoskeletal disorders of the low back are common injuries found in many work environments. Some of the risk factors that workers experience include the weight of object being lifted during a task, frequency, duration, posture, distance the object is lifted, how well the worker can grasp the object, and the degree to which the worker may have to twist and turn their body during a lift. There are many tools that have been developed that are used to assess risk of a worker developing low back pain. The purpose of this study is to determine if the UAW-Ford Ergonomic Surveillance …

Aligning Voice And Communication With Identity – A Survey On Transgender And Gender Diverse Populations, Rachel T. Chalom

USF Tampa Graduate Theses and Dissertations

Purpose: This research has two goals. The first goal is to examine transgender (TG) and gender diverse (GD) voice and communication functioning and the impact of voice on their everyday lives. The second goal is to examine the knowledge, attitudes, and beliefs speech-language pathologists (SLP) have about the TG and GD community and to investigate the relationship between self- and listener-perception and its correspondence to quality of life (QoL).

Methods: A total of 59 participants took part in this research. The research was separated into two parts, the first included 24 TG and GD individuals who participated in a Qualtrics …

The Role Of Threat And Efficacy In Anti-Vaping Ads: A Test Of The Extended Parallel Process Model, Ryan Noone

USF Tampa Graduate Theses and Dissertations

Often thought of as a safer alternative to traditional cigarettes, electronic cigarette use among youth and young adults has steadily increased over the past 10 years. With over 34 percent of high school students and over 7.8 percent of young adults using electronic cigarettes, organizations like the CDC and the FDA have created campaigns and advertisements to combat the epidemic (Truth Initiative). This study uses a 2x2 between subject factoral experiment to gain insights into how varying levels of anti-vaping advertisements’ threat and efficacy elements effect college age students’ perceptions and behaviors towards e-cigarettes. While several of the study’s hypotheses …

Impact Of Heat-Related Illness And Natural Environments On Behavioral Health Related Emergency And Hospital Utilization In Florida, Natasha Kurji

USF Tampa Graduate Theses and Dissertations

Behavioral health disorders are the leading cause of disability in the United States and are known to have multidimensional effect on wellbeing. Several environmental factors are known to impact behavioral health such as weather (i.e. heat) and access to natural environments (i.e. parks and beaches). The study goals were to identify contextual factors that increase the co-occurrence of heat-related illness and behavioral health disorders, illustrate the re-utilization patterns of these co-occurring cases in Florida emergency and inpatient settings, and explore the association between behavioral health disorders such as anxiety and depression, and natural environments such as parks and beaches. The …