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Full-Text Articles in Medicine and Health Sciences
Influences Of Cumulus Cells During In Vitro Fertilization Of Mouse Oocytes In Different Mouse Strains, Haydar Bağiş, Levent Keski̇ntepe, Hakan Sağirkaya, Hande Odaman Mercan, Seyfetti̇n Çeti̇n
Influences Of Cumulus Cells During In Vitro Fertilization Of Mouse Oocytes In Different Mouse Strains, Haydar Bağiş, Levent Keski̇ntepe, Hakan Sağirkaya, Hande Odaman Mercan, Seyfetti̇n Çeti̇n
Turkish Journal of Veterinary & Animal Sciences
In this study, the influences of cumulus cells during in vitro fertilization (IVF) and their subsequent effects on mouse embryo development in vitro were studied in inbred and hybrid strain mice. Embryos at the 2-cell stage obtained after in vitro fertilization were transferred to the oviduct of foster mothers to assess their implantation rate. Mouse oocytes, which were intact at the time of in vitro fertilization, demonstrated less polyspermic fertilization and more blastocyst development than those inseminated without any cumulus cells. The data demonstrate that more oocytes were arrested at the 2-cell stage in groups that contained no cumulus cells. …
Effect Of Czb Medium On The Two Cell Block Of Preimplantation Mouse Emryos, Haydar Bağiş, Levent Keski̇ntepe, Hande Odaman Mercan, Hakan Sağirkaya
Effect Of Czb Medium On The Two Cell Block Of Preimplantation Mouse Emryos, Haydar Bağiş, Levent Keski̇ntepe, Hande Odaman Mercan, Hakan Sağirkaya
Turkish Journal of Veterinary & Animal Sciences
This experiment was designed to determine the effects of CZB medium on in vitro culture of murine embryos obtained from FVB/N, CD-1, and CB6F1 strains. Pronuclear stage embryos were recovered from superovulated and mated females 17 h post-hCG. Two-cell stage block, morula and blastocyst stages were used as the end point of the experiments. There is no statistical difference among strains and media for the development to the two-cell stage. However, more FVB/N embryos (88.3 and 82.4%) than CD-1 (57.4 and 57.1%) and CB6F1 (69.8 and 28.9%) reached the 3-8 cell stage in CZB and M16 media, respectively. CB6F1 embryos …