Open Access. Powered by Scholars. Published by Universities.®

Medicine and Health Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 30 of 97

Full-Text Articles in Medicine and Health Sciences

Characterization Of Berberine On Human Cancer Cells In Culture, Savio Szeto, Christine Miu Ngan Yow, Ka Wah Fung Jan 2002

Characterization Of Berberine On Human Cancer Cells In Culture, Savio Szeto, Christine Miu Ngan Yow, Ka Wah Fung

Turkish Journal of Medical Sciences

Berberine originates from a Chinese herbal medicine and possesses a wide variety of anti-cancer activities. In this study, the killing effect of berberine on nasopharyngeal carcinoma cells (NPC/HK1) was investigated. The trypan blue exclusion assay was used to assess the cytotoxic effect of berberine in this cell line. Berberine, at 5-200 µM, induced cell death in a dose-dependent manner. Treatment of cells with 200 µM berberine for 5 h yielded a lethal dose of 50% (LD50). The Comet Assay was employed to evaluate the extent of DNA damage and repair after berberine treatment (0 -100 µM). DNA damage was evident …


Accidental Displacement Of Impacted Third Molar Into Lateral Pharyngeal Space, Keri̇m Ortakoğlu, Kemal Murat Okçu, Hakan Alpay Karasu, Yilmaz Günaydin Jan 2002

Accidental Displacement Of Impacted Third Molar Into Lateral Pharyngeal Space, Keri̇m Ortakoğlu, Kemal Murat Okçu, Hakan Alpay Karasu, Yilmaz Günaydin

Turkish Journal of Medical Sciences

TAKOĞLU^1 Kemal Murat OKÇU1 Hakan Alpay KARASU^2 Yılmaz GÜNAYDIN^1 ^1Department of Oral and Maxillofacial Surgery, Dental Sciences Center, Gülhane Military Medical Academy, Etlik, Ankara, ^2Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Ankara University, Beşevler, Ankara - Turkey


Oxidative Stress In Mothers And Their Newborns In Different Types Of Labour, İdri̇s Mehmetoğlu Akkuş, Ali̇ Kart, Osman Çağlayan, Meti̇n Çapar, Recep Gökçe Jan 2002

Oxidative Stress In Mothers And Their Newborns In Different Types Of Labour, İdri̇s Mehmetoğlu Akkuş, Ali̇ Kart, Osman Çağlayan, Meti̇n Çapar, Recep Gökçe

Turkish Journal of Medical Sciences

HMETOĞLU (AKKUŞ)^1 Ali KART^1 Osman ÇAĞLAYAN^1 Metin ÇAPAR^2 Recep GÖKÇE^1 Departments of 1Biochemistry, 2Obstetrics and Gynaecology, Faculty of Medicine, Selçuk University. Konya - Turkey


A Simple Comparison Between Simultaneous Kidney-Pancreas (Skptx) And Kidney-Alone (Ktx) Transplants In Type I Diabetic Nephropaty, Tanju Acar, Arshad Mahmood Jan 2002

A Simple Comparison Between Simultaneous Kidney-Pancreas (Skptx) And Kidney-Alone (Ktx) Transplants In Type I Diabetic Nephropaty, Tanju Acar, Arshad Mahmood

Turkish Journal of Medical Sciences

Simultaneous kidney-pancreas transplantation (SKPtx) is an established procedure at many transplant centres for the treatment of end-stage renal disease from type I diabetes mellitus. A successful transplant normalises glucose metabolism and may prevent or reverse some of the complications of diabetes. Concerning graft survival and morbidity rates, the usefulness of this procedure is still questioned by some authors (1,2). We undertook a retrospective study to investigate the differences between survival rates of SKPtx and kidney alone transplant (Ktx) recipients.We also evaluated morbidity rates and complications in both groups. There were no significant differences in 12 and 36 month survival rates. …


Papillary Adenocarcinoma Of The Epiglottis, Sema Hücümenoğlu, Nur Çeli̇k, Gülben Erdem, Si̇nan Kocatürk Jan 2002

Papillary Adenocarcinoma Of The Epiglottis, Sema Hücümenoğlu, Nur Çeli̇k, Gülben Erdem, Si̇nan Kocatürk

Turkish Journal of Medical Sciences

ÜMENOĞLU^1 Nur ÇELİK^1 Gülben ERDEM^1 Sinan KOCATÜRK^2 Departments of ^1Pathology, ^2Otorhinolaryngology, SSK Training and Research Hospital, Ankara, Turkey


The Effects Of Three Neurosurgical Anaesthetic Methods On Hla-Dr Expression, Nusa Tuna, Meti̇n Tuna, Demet Koca, Şebnem Atici, Aniş Ariboğan Jan 2002

The Effects Of Three Neurosurgical Anaesthetic Methods On Hla-Dr Expression, Nusa Tuna, Meti̇n Tuna, Demet Koca, Şebnem Atici, Aniş Ariboğan

Turkish Journal of Medical Sciences

Expression of human leukocyte antigen-DR (HLA-DR) is considered a meaningful indicator of immune response because of the important role that HLA-DR plays in presenting antigen to T-lymphocytes. The aim of this study was to evaluate the effects of three anaesthetic methods (intravenous anaesthesia with propofol-fentanyl and balanced anaesthesia with isoflurane-fentanyl or sevoflurane-fentanyl) on monocytic HLA-DR expression in neurosurgical patients who underwent craniotomy. Thirty patients were divided into 3 groups of 10, and each group was the subject of one of the anaesthetic regimes noted above during craniotomy procedures. Blood samples were collected and HLA-DR expression was measured by flow cytometry …


Arsenic Trioxide Has Additive Cytotoxic Effects On Mcf-7 Breast Cancer Cell Line With Taxanes, Canfeza Sezgi̇n, Ulus Ali̇ Şanli, Rüçhan Uslu, Erdem Göker Jan 2002

Arsenic Trioxide Has Additive Cytotoxic Effects On Mcf-7 Breast Cancer Cell Line With Taxanes, Canfeza Sezgi̇n, Ulus Ali̇ Şanli, Rüçhan Uslu, Erdem Göker

Turkish Journal of Medical Sciences

Breast cancer is the most prevalent cancer in women and an important cause of both morbidity and mortality. Although long-term disease-free survival is offered to women with early stage disease by current treatment modality, some, 30-40% of patients will have metastatic disease. Treatment of patients with metastatic disease is a great challenge for oncologists. Therefore, new drugs and therapeutic options for women with metastatic breast cancer are urgently required. Arsenical compounds have cytotoxic effects on several cancer cell lines including promyelocytic leukemia, esophageal carcinoma, megakaryocytic leukemia, and malignant lymphocytic cell lines. In addition, recent studies showed that arsenic trioxide (As_2O_3) …


Bile Duct Response To Repeated Cerulein Induced Pancreatitis In The Rat, Ali̇ Menteş, Hasan Aydede, Ümi̇t Bayol, Funda Yilmaz Jan 2002

Bile Duct Response To Repeated Cerulein Induced Pancreatitis In The Rat, Ali̇ Menteş, Hasan Aydede, Ümi̇t Bayol, Funda Yilmaz

Turkish Journal of Medical Sciences

The typical morphology of common bile duct strictures in chronic pancreatitis suggests a pathogenesis not of encasement, but of mural changes in the common bile duct. Cerulein, 20 µg/kg was administered to male Wistar rats in four subcutaneous doses, hourly for 3 h, and was recorded as one application. Group A served as controls and received subcutaneous saline. Group B1 received one application of Cerulein and was sacrificed after 24 h. Group B2 was similar to B1, but was sacrificed two weeks later. Group B3 was given two applications of Cerulein with an interval of two weeks and was sacrificed …


Effects Of Short-Term Orlistat Therapy On Maximal Power Production Capacity In Obese Patients, Oğuz Özçeli̇k, Rami̇s Çolak, A. Baki̇ Türkoğlu Jan 2002

Effects Of Short-Term Orlistat Therapy On Maximal Power Production Capacity In Obese Patients, Oğuz Özçeli̇k, Rami̇s Çolak, A. Baki̇ Türkoğlu

Turkish Journal of Medical Sciences

The aim of this study was to examine the effects of weight loss induced by hypocaloric diet and orlistat therapy for a four-week period on maximal work rate (Wmax) production capacity and aerobic fitness in obese patients. Fifteen obese subjects were given an integrated energy restricted diet and orlistat supplement (DO). Each patient performed two incremental ramp exercise tests using an electromagnetically braked cycle ergometer: one at the beginning and one at the end of the four-weeks to determine Wmax production capacity. Four weeks of DO therapy resulted in a significant reduction in total body weight (93.7 ± 14.6 kg …


The Effects Of Pentoxifylline In Experimental Nerve Injury, Süleyman Baykal, Cavi̇t Boz, Ertuğrul Çakir, Şükrücan H. Baytan, Murat Karakuş, Kayhan Kuzeyli̇ Jan 2002

The Effects Of Pentoxifylline In Experimental Nerve Injury, Süleyman Baykal, Cavi̇t Boz, Ertuğrul Çakir, Şükrücan H. Baytan, Murat Karakuş, Kayhan Kuzeyli̇

Turkish Journal of Medical Sciences

Several studies have suggested that pentoxifylline, a theophylline-related drug that decreases erytrhocyte stiffness, may be useful in treatment of ischaemia reperfusion injuries of brain, liver and small intestine in animal experiments. Fourteen Wistar-Albino rats weighing between 180-250 g were used for the experiment and divided equally to two groups. Before the injury, electroneuromyographic (ENMG) data were recorded from right and left sciatic nerves. Right sciatic nerves of the seven rats of group 1 were crushed with a clip. Same lesions were performed on seven rats of group 2 and also pentoxifylline (50mg/kg/day) was applied intraperitoneally once in a day for …


Analyses Of Surface Dose From High Energy Photon Beams For Different Clinical Setup Parameters, Nadi̇r Küçük, Ayhan Kiliç, Gönül Kemi̇kler, Lütfi̇ Özkan, Kayihan Engi̇n Jan 2002

Analyses Of Surface Dose From High Energy Photon Beams For Different Clinical Setup Parameters, Nadi̇r Küçük, Ayhan Kiliç, Gönül Kemi̇kler, Lütfi̇ Özkan, Kayihan Engi̇n

Turkish Journal of Medical Sciences

Surface and build-up region doses of 6, 15 and 25 MV photon beams were investigated for several clinical setup parameters. Evaluated setups included open fields, physical wedge (PW) fields, virtual wedge (VW) fields and blocked fields. The effects of field size, acrylic block tray, source-to-surface distance (SSD) and off-axis distance on surface dose were determined for each of these setups. Siemens Mevatron MD2 (6-15 MV) and Mevatron-KD2 (6-25 MV) linear accelerators were used to measure both the surface and build-up region doses. The surface dose increased as field size increased in open fields. There was no significant difference between 6 …


Genetic Polymorphisms In Steroid Hormone Metabolizing Enzymes In Human Breast Cancer, Nesli̇han Aygün Kocabaş Jan 2002

Genetic Polymorphisms In Steroid Hormone Metabolizing Enzymes In Human Breast Cancer, Nesli̇han Aygün Kocabaş

Turkish Journal of Medical Sciences

Epidemiologic studies indicate that most risk factors for breast cancer are related to reproductive and hormonal factors. The evaluation of associations between breast cancer risk and genetic polymorphisms in enzymes involved in hormone metabolism may be a cost effective manner in which to determine individual breast cancer susceptibility. A number of molecular epidemiologic studies have been conducted to evaluate associations between polymorphic genes involved in steroid hormone metabolism (i.e., CYP17, CYP19, CYP1A1, CYP1B1, MnSOD, COMT, and GST) that may account for a proportion of enzymatic variability. An evaluation of associations between breast cancer risk and genetic polymorphisms in enzymes involved …


Detection Of Human Papillomavirus Infection In Esophageal Carcinomas By The Histopathological Method And Polymerase Chain Reaction Technique, İlhami̇ Ki̇ki̇, Mehmet Gündoğdu, Muhammet Fevzi̇ Polat, Cemal Gündoğdu Jan 2002

Detection Of Human Papillomavirus Infection In Esophageal Carcinomas By The Histopathological Method And Polymerase Chain Reaction Technique, İlhami̇ Ki̇ki̇, Mehmet Gündoğdu, Muhammet Fevzi̇ Polat, Cemal Gündoğdu

Turkish Journal of Medical Sciences

A series of 30 surgical resection materials of patients with squamous cell carcinoma (SCC) and 10 with adenocarcinoma (AC) were analyzed histologically and by polymerase chain reaction (PCR) to demonstrate whether human papillomavirus (HPV) has a role in the etiology of esophageal cancers. Epithelial changes suggesting HPV infection were histopathologically detected in 19 of 30 (63.3%) SCC and in 4 of 10 (40%) AC cases. By PCR, HPV type 16 and/or type 18 DNA were detected in 10 of 30 (33.3%) SCC and HPV type 18 DNA alone was detected in 1 of 10 (10%) AC cases. Histopathologic findings related …


Intraarticular Cyst Of The Knee, Gülay Yeği̇noğlu, Osman Aynaci, Ahmet Uğur Turhan Jan 2002

Intraarticular Cyst Of The Knee, Gülay Yeği̇noğlu, Osman Aynaci, Ahmet Uğur Turhan

Turkish Journal of Medical Sciences

GİNOĞLU^1 Osman AYNACI^2 Ahmet Uğur TURHAN^2 Department of ^1Anatomy, ^2Orthopedic Surgery, School of Medicine, Karadeniz Technical University, 61080 Trabzon - Turkey


Distribution Of Different Fibronectin Isoforms In The Extracellular Matrices Of Human Term Placenta, Ayşe Yasemi̇n Demi̇r Weusten Jan 2002

Distribution Of Different Fibronectin Isoforms In The Extracellular Matrices Of Human Term Placenta, Ayşe Yasemi̇n Demi̇r Weusten

Turkish Journal of Medical Sciences

Fibronectins are considered to be molecules that play important roles in cell migration and cell differentiation. Fibronectin molecules are thought to influence the differentiation of trophoblasts and the adhesion of these cells to maternal tissues. The aim of the present study was to analyze the distribution of fibronectin isoforms and to evaluate the differences between matrix-type fibrinoids, and other types of matrices produced by both maternal and fetal tissues. Tissue samples were collected from different sites of human term placentas. By using immunohistochemistry, the expression of fibronectin isoforms was determined and they were demonstrated by SDS-PAGE and Western blotting methods. …


The Problem Of Lipid Exchange In Vessel Walls In Insulin Deficit, Gulchekhra M. Irgasheva, Lyudmila Sergeevna Klemesheva, Karim Tadjibaevich Almatov Jan 2002

The Problem Of Lipid Exchange In Vessel Walls In Insulin Deficit, Gulchekhra M. Irgasheva, Lyudmila Sergeevna Klemesheva, Karim Tadjibaevich Almatov

Turkish Journal of Medical Sciences

It has been revealed that in alloxane diabetes the content of the main phospholipids - phosphatidylcholine, phosphatidyletanolamine and phosphatidyl-phosphatidylinosite -decreases in the cells of the vascular wall, whereas the content of the methyl ethers of the fatty acids is not changed, and the content of the fractions of lipids (monoglycerides, diglycerides, triglycerides, cholesterol, free fatty acids, and cholesterol ethers) and phospholipids (phosphatidylserine, cardiolipin, lysophosphatidyletanolamine, lysocardiolipin, sphingomyelin, phosphatide acid and lysophosphatide acid) increase. The depth of these changes correlates with the heaviness of the pathological process.


Rate-Dependent Effects Of Dofetilide On Epicardial Monophasic Action Potentials In Isolated Rabbit Heart With Atrial Pacing, Mustafa Özbek, Şule Gök Jan 2002

Rate-Dependent Effects Of Dofetilide On Epicardial Monophasic Action Potentials In Isolated Rabbit Heart With Atrial Pacing, Mustafa Özbek, Şule Gök

Turkish Journal of Medical Sciences

Dofetilide (UK 68,798), a new antiarrhythmic agent, blocks potassium channels selectively and acts primarily by prolonging repolarization duration. The aim of this study was to investigate rate-dependent repolarization changes due to dofetilide in a fast beating in-vitro heart model. The study was designed using Langendorff perfusion of rabbit hearts at the drug concentrations of 1 nM, 3 nM and 10 nM. The electrophysiological evaluations were performed using the recording of epicardial monophasic action potentials. Atrial pacing was set at the cycle length (ms) of 300, 325, 350, 375 and 400 so that repolarization duration could be measured in different heart …


Colicinogeny, Lipopolysaccharide And Outer Membrane Protein Profiles Of Multi-Drug Resistant Salmonella Typhimurium Isolates From Turkey, Göksu Elpek, Bülent İçgen, Gülay Özcengi̇z Jan 2002

Colicinogeny, Lipopolysaccharide And Outer Membrane Protein Profiles Of Multi-Drug Resistant Salmonella Typhimurium Isolates From Turkey, Göksu Elpek, Bülent İçgen, Gülay Özcengi̇z

Turkish Journal of Medical Sciences

A total of 259 clinical isolates of non-repetitive non-typhi salmonellae (NTS) were previously examined for their antibiotic resistance patterns and plasmid contents. Multi-drug resistant strains comprised 19.3% (50/259) of the isolates and almost all were Salmonella typhimurium. In the present study, 35 of these multi-drug resistant S. typhimurium isolates were further characterized for colicinogeny, lipopolysaccharide (LPS) and outer membrane protein (OMP) profiles. Fourteen of the 35 (40%) isolates were found to be colicin producers. All isolates showed smooth-type LPS. In 26 of the (74.3%) isolates, OMPs of 30.6 and 34.6 kDa were observed whereas three (8.6%) of them were found …


Clinical Evaluation Of The Fastplaquetb For The Rapid Diagnosis Of Pulmonary Tuberculosis, Cengi̇z Çavuşoğlu, Sevi̇nç Güneri̇, Mutay Suntur, Altinay Bi̇lgi̇ç Jan 2002

Clinical Evaluation Of The Fastplaquetb For The Rapid Diagnosis Of Pulmonary Tuberculosis, Cengi̇z Çavuşoğlu, Sevi̇nç Güneri̇, Mutay Suntur, Altinay Bi̇lgi̇ç

Turkish Journal of Medical Sciences

The reliability of the FASTPlaqueTB (FPTB) test (Biotec Ltd.) for the rapid diagnosis of pulmonary tuberculosis was evaluated and its diagnostic performance was compared with the Amplified Mycobacterium Tuberculosis Direct (MTD) test (Gen-Probe) by testing 80 sputum samples obtained from 77 patients. The results were compared to those of mycobacterial culture, smear and clinical course. After a chart review 14 culture-negative patients who had been on antituberculosis therapy before the study began were excluded from the final analysis. Of the remaining 63 patients, 33 were considered to have tuberculosis; 29 of them were both smear and culture positive, for whom …


Penetrating Cardiac Injuries, Ahmet Özyazicioğlu, Azman Ateş, Münacetti̇n Cevi̇z, Sami̇ Karapolat, Engi̇n Bozkurt, Hi̇kmet Koçak Jan 2002

Penetrating Cardiac Injuries, Ahmet Özyazicioğlu, Azman Ateş, Münacetti̇n Cevi̇z, Sami̇ Karapolat, Engi̇n Bozkurt, Hi̇kmet Koçak

Turkish Journal of Medical Sciences

Objectives: To present our experience of penetrating cardiac injuries treated at Atatürk University hospital; in 17 years 38 patients were analyzed. Methods: Patients were classified into three groups: group A (stable), 12; group B (shock), 21; and group C (agonal), five. Five patients were treated by pericardial window and three by pericardiocentesis. Two patients in group C, 19 patients in group B and five patients in group A underwent median sternotomy or thoracotomy in the operating room. Emergency room thoracotomies were performed in four patients. Results: There were 12 patients in group A, of which 11 survived (92 %). Of …


Nondecidualized And Decidualized Endometriosis Of The Abdominal Wall (A Report Of Two Cases Secondary To Cesarean Section), Ni̇hal Kilinç, Ahmet Yalinkaya, Mehmet Özaydin Jan 2002

Nondecidualized And Decidualized Endometriosis Of The Abdominal Wall (A Report Of Two Cases Secondary To Cesarean Section), Ni̇hal Kilinç, Ahmet Yalinkaya, Mehmet Özaydin

Turkish Journal of Medical Sciences

LINÇ Ahmet YALINKAYA Mehmet ÖZAYDIN Department of Pathology, Faculty of Medicine, Dicle University, Diyarbakır - TURKEY


An Alternative Method To The Volume Estimation Of Cavities With Irregular Surface In The Cadaver: Measuring Right Cardiac Ventricle Of The Fetus By Using Mercury, Zeli̇ha Kurtoğlu, M. Haluk Uluutku Jan 2002

An Alternative Method To The Volume Estimation Of Cavities With Irregular Surface In The Cadaver: Measuring Right Cardiac Ventricle Of The Fetus By Using Mercury, Zeli̇ha Kurtoğlu, M. Haluk Uluutku

Turkish Journal of Medical Sciences

To find the volume of irregular cavities in cadavers, many researchers have used different methods including filling with any fluid and calculating the volume by usingcomplex geometrical formulas. All of these have some difficulties and disadvantages. In this paper, filling cavities with mercury is presented as an alternative method for volume estimation of the cavities. The volumes of 22 right cardiac ventricles were found by using mercury in 27-34 week-old fetuses. The method was proposed as an easy and sensitive method for volume estimation and comparison of the cavities in the cadaver.


Invasive Pulmonary Aspergillosis In Solid-Organ Transplant Recipients: Postmortem Histopathologic Findings, Banu Bi̇lezi̇kçi̇, Beyhan Demi̇rhan, Asuman Ni̇han Haberal, Ünser Arikan Jan 2002

Invasive Pulmonary Aspergillosis In Solid-Organ Transplant Recipients: Postmortem Histopathologic Findings, Banu Bi̇lezi̇kçi̇, Beyhan Demi̇rhan, Asuman Ni̇han Haberal, Ünser Arikan

Turkish Journal of Medical Sciences

Invasive aspergillosis is often lethal in transplant recipients. The mortality associated with this disease after organ transplantation approaches 100%, partly because it is difficult to recognize and diagnose early on. The clinical course and histological findings in invasive pulmonary aspergillosis in immunocompromised hosts differ from those seen in patients who develop classic aspergillomas. In immunosuppressed patients, the predominant pathologic abnormalities are tissue invasion, abscess formation, and angioinvasion with or without infarction. In this study, we examined cases of invasive pulmonary aspergillosis in one kidney recipient and three liver transplant recipients. All the patients were male, and their mean age was …


Effects Of Increased Respiratory Resistance On Maximal O_2 Uptake And Anaerobic Threshold During Incremental Exercise Tests, Oğuz Özçeli̇k, Rami̇s Çolak Jan 2002

Effects Of Increased Respiratory Resistance On Maximal O_2 Uptake And Anaerobic Threshold During Incremental Exercise Tests, Oğuz Özçeli̇k, Rami̇s Çolak

Turkish Journal of Medical Sciences

This study was undertaken to assess whether maximal O_2 uptake (VO_2max) can be used as an index of measuring aerobic capacity under the condition of increased respiratory resistance. Seven male subjects performed two incremental exercise tests on a cycle ergometer on different days: one control (C) and one breathing through an 8 mm bore diameter respiratory resistance (R). Ventilatory and gas exchange responses were measured with a turbine volume transducer and mass spectrometry, and processed breath-by-breath. VO_2max was measured and anaerobic threshold (AT) was estimated non-invasively using the V-slope method. Maximal exercise performance was reduced significantly (by paired t-test, P


A Rare Case Of Neonatal Hypotonia: Neonatal Nonketotic Hyperglycinemia, Ayhan Taşteki̇n, Rahmi̇ Örs, Behzat Özkan, Orhan Deni̇z Jan 2002

A Rare Case Of Neonatal Hypotonia: Neonatal Nonketotic Hyperglycinemia, Ayhan Taşteki̇n, Rahmi̇ Örs, Behzat Özkan, Orhan Deni̇z

Turkish Journal of Medical Sciences

Hypotonia is a serious physical finding in a newborn infant. Nonketotic hyperglycinemia (NKH) is a rare cause of neonatal hypotonia. It is a defect of the glycine metabolism and has poor prognosis. We report a case of a newborn infant with NKH, presented with mild hypotonia and high creatine kinase values. NKH should be considered in a hypotonic newborn, especially if associated with acute deterioration, lethargy and seizures.


Congenital Pseudoarthrosis Of The Clavicle With Multiple Vertebral Anomalies, Ahmet Yi̇ği̇t Göktay, Mustafa Seçi̇l, Erki̇n Çeti̇nkol, Di̇nç Özaksoy Jan 2002

Congenital Pseudoarthrosis Of The Clavicle With Multiple Vertebral Anomalies, Ahmet Yi̇ği̇t Göktay, Mustafa Seçi̇l, Erki̇n Çeti̇nkol, Di̇nç Özaksoy

Turkish Journal of Medical Sciences

ğit GÖKTAY Mustafa SEÇİL Erkin ÇETİNKOL Dinç ÖZAKSOY Department of Radiology, Faculty of Medicine, Dokuz Eylül University, İzmir - TURKEY


Production And Purification Of Monoclonal Antibodies To Human Interleukin-2 And Their Use For Immunoaffinity Purification Of Recombinant Human Interleukin-2, Cemaletti̇n Aybay Jan 2002

Production And Purification Of Monoclonal Antibodies To Human Interleukin-2 And Their Use For Immunoaffinity Purification Of Recombinant Human Interleukin-2, Cemaletti̇n Aybay

Turkish Journal of Medical Sciences

Monoclonal antibodies (mAbs) are a powerful immunochemical tool. Today, the availability of mAbs with desired specificity enables ligand separation based upon the immunoaffinity technique. By using the high selectivity of mAbs, immunoaffinity purification offers the possibility of isolating compounds even from complex samples with a selectivity which cannot be achieved by other methods. This paper reports the preparation and identification of monoclonal antibodies against human interleukin-2 (IL-2) and the establishment of an immunoaffinity method for purifying human IL-2 (hIL-2). Hybridomas were generated by the fusion of NSO or FO myelomas and spleen cells from immunized mice. Hypoxanthine-aminopterin-thymidine-resistant hybridomas secreting antibodies …


The Relation Between Low-Density Lipoprotein (Ldl) Oxidation And Hemodialysis With Respect To Membrane Types Protective Effects Of Vitamin E-Bonded Membrane, Selda Demi̇rtaş, Gökhan Nergi̇soğlu, Ayşegül Akbay Yarpuzlu, Levent Karaca Jan 2002

The Relation Between Low-Density Lipoprotein (Ldl) Oxidation And Hemodialysis With Respect To Membrane Types Protective Effects Of Vitamin E-Bonded Membrane, Selda Demi̇rtaş, Gökhan Nergi̇soğlu, Ayşegül Akbay Yarpuzlu, Levent Karaca

Turkish Journal of Medical Sciences

Plasma total antioxidant (TAO) levels, LDL (low density lipoprotein), antioxidant defense potential (AOP) and the resistance to oxidation of LDL fractions were studied in chronic hemodialysis patients. The differences with respect to dialyzer membrane types before and after hemodialysis were also assessed. After isolation of LDL from plasma by precipitation, AOP was measured based on the determination of TBARS (thiobarbituric acid reactive substance), and resistance to oxidation was assessed by the metal-catalyzed peroxidation of LDL followed by the determination of the values of the peroxidation product malondialdehide (MDA) before and after peroxidation. The assay principle of TAO was based on …


Effect Of Low Dietary Zinc Intake And Experimental Diabetes On The Zinc And Carbohydrate Metabolism In Rats, Zine Kechrid, Nazan Demi̇r, Cherif Abdennour, Noureddine Bouzerna Jan 2002

Effect Of Low Dietary Zinc Intake And Experimental Diabetes On The Zinc And Carbohydrate Metabolism In Rats, Zine Kechrid, Nazan Demi̇r, Cherif Abdennour, Noureddine Bouzerna

Turkish Journal of Medical Sciences

To investigate the effect of low dietary zinc intake and experimental diabetes (IDDM) on the zinc and carbohydrate metabolism, 8-week-old male wealing normal albino (Wistar) rats were fed diets containing either adequate (54mg/kg) or low zinc (1mg/kg) quantities for one week. Ten rats from each group (n=20) were then intraperitoneally injected with alloxan to induce diabetes. The rats were sacrificed after a further three weeks. Body weight gain and food intake were recorded regularly. On day 28, after an overnight fast, the animals were sacrificed and blood glucose, serum insulin, serum cholesterol concentrations, liver glycogen contents, and femur and pancreatic …


Microscopy Of Rat Thoracic Aorta In Chronic Ethanol Intake, Feral Öztürk, Ni̇gar Vardi, Nureddi̇n Cengi̇z, Mukaddes Eşrefoğlu, Ali̇ Otlu Jan 2002

Microscopy Of Rat Thoracic Aorta In Chronic Ethanol Intake, Feral Öztürk, Ni̇gar Vardi, Nureddi̇n Cengi̇z, Mukaddes Eşrefoğlu, Ali̇ Otlu

Turkish Journal of Medical Sciences

The aim of this study was to determine the histological changes of the thoracic aorta in rats fed on alcohol for a period of 24 weeks. Twenty male Wistar rats were divided into two groups. The ethanol group (n=10) was fed on a modified liquid diet (MLD) containing 7.2% ethanol for 24 weeks. The control group (n=10) was fed on MLD without ethanol throughout the experiment. At the end of the 24 weeks, the carotid arteries of the rats were cannulated and their blood pressures were measured. Ethanol levels measured by using the NAD+/NADH enzyme-spectrophotometric method. Rats were killed by …