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Difficulties Associated With The Study Of Action Potential After-Potentials In Rat Cultured Sensory Neurones, Ahmet Ayar, Roderick H. Scott

Turkish Journal of Medical Sciences

We investigated the properties of action potential after-potentials in cultured dorsal root ganglion neurones from neonatal rats using the whole cell patch clamp technique. The aims of this study were to characterize after-potentials and investigate the possible influences of Ca^{2+}-induced Ca^{2+}release (CICR) on neuronal excitability. The reversal potentials of action potential after-depolarizations and after-hyperpolarizations were estimated. Although evidence for both Ca^{2+}-activated chloride and potassium conductances was obtained, the data indicated that the after-potentials were contaminatied by a variety of distinct ionic events. Apamin was used to attenuate potassium conductances and appeared to enhance the after-depolarizations due to Ca^{2+}-activated chloride conductances. …