Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Medicine and Health Sciences
Excisional Wound Healing And The Role Of Homeobox Genes, David Allen Lanning
Excisional Wound Healing And The Role Of Homeobox Genes, David Allen Lanning
Theses and Dissertations
The biochemical and physiologic mechanisms responsible for excisional wound contraction have been extensively studied but are not completely understood. Furthermore, the genes that regulate these processes are unknown. As a result, there are limited treatment options available for patients with diseases or conditions characterized by abnormal tissue contraction. In an attempt to improve our understanding of these mechanisms and identify some of the putative regulatory genes, we studied excisional wound healing in the mid-gestational fetal rabbit. These wounds normally do not contract nor are they associated with inflammation, fibrosis, or scar formation. However, we have been able to induce contraction, …
Study Of P53 Gain Of Function Mutations In P53-Null Astrocytes, Sang H. Choi
Study Of P53 Gain Of Function Mutations In P53-Null Astrocytes, Sang H. Choi
Theses and Dissertations
A number of recent studies suggest that expression of mutant p53 with mutations in certain codons show a gain of function and some of the characteristics of an oncoprotein. In order to study gain of function mutation and eliminate the potential of a dominant negative interaction with endogenous wild type p53 protein, p53 knockout mouse astrocytes were used. A retrovirus system was used to introduce mutant p53 genes into these p53-null astrocytes. Immunohistochemical staining and western blot experiments showed the expression of mutant p53 protein in these cells after infection with the mutant p53 retroviruses. Cell growth experiment did not …