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Full-Text Articles in Medicine and Health Sciences
E-Cadherin Localization In Oviduct And Uterine Horn Of Swamp Buffalo During Estrous Cycle, Paisan Tienthai
E-Cadherin Localization In Oviduct And Uterine Horn Of Swamp Buffalo During Estrous Cycle, Paisan Tienthai
The Thai Journal of Veterinary Medicine
The main purpose of the present study was to investigate the localization of epithelial cadherin (E-cadherin) in the oviduct and uterine horn of swamp buffalo at the follicular and mid-luteal phases. E-cadherin is a transmembrane glycoprotein which plays a vital role in the mechanism of the maintenance epithelial architecture, cell growth and proliferation, cell to cell adhesion including cell to epithelium adhesion. Female reproductive tracts of swamp buffaloes (n=30) were collected from local abattoirs and categorized into follicular (n=15) and mid-luteal (n=15) phases. Tissue samples from the oviducts and uterine horns were regularly handled according to histological and immunohistochemical techniques. …
Morphological Aspects By Light And Scanning Electron Microscopic Studies Of Swamp Buffalo Endometrium At Follicular And Mid-Luteal Phases, Paisan Tienthai, Kriengyot Sajjarengpong
Morphological Aspects By Light And Scanning Electron Microscopic Studies Of Swamp Buffalo Endometrium At Follicular And Mid-Luteal Phases, Paisan Tienthai, Kriengyot Sajjarengpong
The Thai Journal of Veterinary Medicine
This study was carried out to observe morphological changes of swamp buffalo endometrium at follicularand mid-luteal phases. Uterine horns were collected from female buffaloes at a local abattoir and the selected estrousstages were categorized into the follicular (n = 10) and mid-luteal (n = 10) phases. General histology andhistomorphometry were examined under light microscope (LM) whereas a scanning electron microscope (SEM) wasused to study surface epithelial changes. The results showed that the height of the endometrial epithelium, thenumber of superficial endometrial glands and the number of capillaries were significantly greater (p < 0.05) at thefollicular phase. By SEM examination, the ciliated and secretory cells with different patterns, i.e. abundant microvillion the apical part or secretory protrusion in various degrees, distinctly increased at the follicular phase. In themeantime, numerous secretory cells with stubby microvilli were covered throughout the endometrial surface withsecretory vesicles on endometrial glandular orifices at the mid-luteal phase in which the ciliated cells were sparselyseen. It was concluded that the swamp buffalo endometrium obviously revealed modifications during the estrouscycle for physiological events, i.e. sperm transport, early embryonic development and implantation.
Reproductive And Dairy Performances Of Thai Swamp Buffaloes Under Intensive Farm Management, Thuchadaporn Chaikhun, Ranchuan Hengtrakunsin, Fabio De Rensis, Mongkol Techakumphu, Siriwat Suadsong
Reproductive And Dairy Performances Of Thai Swamp Buffaloes Under Intensive Farm Management, Thuchadaporn Chaikhun, Ranchuan Hengtrakunsin, Fabio De Rensis, Mongkol Techakumphu, Siriwat Suadsong
The Thai Journal of Veterinary Medicine
In this study the reproductive and dairy performances of swamp buffaloes under intensive farm conditions were evaluated. The data of reproduction and milk production were collected from forty-eight swamp buffaloes during the period of 2001 to 2010. The reproductive and dairy performances were analyzed as mean±SD of each index. The age at first calving was 47.1±8 months and the gestation period was 321.4±11.3 days. The service per conception was 3.5±2.5, with insemination by both natural and artificial mating. The calving interval and the days open were 486.2±75.02 and 166.2±69.7 days (n=99), respectively. Additionally, there was a large increase in primiparous …
Hyaluronan And Syndecan-1 Localization In The Swamp Buffalo Oviduct, Paisan Tienthai
Hyaluronan And Syndecan-1 Localization In The Swamp Buffalo Oviduct, Paisan Tienthai
The Thai Journal of Veterinary Medicine
The glycosaminoglycans (GAGs) found in the mammalian oviductal tissue and luminal secretory fluidinfluence the fertilization processes in various patterns such as sperm storage, viability and capacitation. The aim ofthis study was to confirm the appearance of hyaluronan and syndecan-1 in the swamp buffalo oviduct consisting ofuterotubal junction (UTJ), isthmus, ampulla and infundibulum during oestrous cycle using histochemical andimmunohistochemical techniques. The non-sulphated GAGs, i.e. hyaluronan (HA), were noticeably localized in thesubepithelial connective tissue layer or lamina propria of all segments while the epithelial HA-positive staining wasdemonstrated only in the UTJ and isthmus (sperm reservoir) at the follicular phase. In contrast, the …
Fas Ligand In Swamp Buffalo Oviduct During Follicular And Luteal Phases, Paisan Tienthai, Pongpisut Chivacharern, Kriengyot Sajjarengpong, Pornchalit Assavacheep
Fas Ligand In Swamp Buffalo Oviduct During Follicular And Luteal Phases, Paisan Tienthai, Pongpisut Chivacharern, Kriengyot Sajjarengpong, Pornchalit Assavacheep
The Thai Journal of Veterinary Medicine
Fas ligand (FasL) and its receptor (Fas) are tumor necrosis factor (TNF) members which are involved in theimmune privileged organs, such as the cornea, testis and placenta, by triggering apoptosis in various cell types. Theappearance of a Fas-FasL system might specify the immune privileged status of the buffalo oviduct wherespermatozoa avoid eradication by female immune cells. The objective of this study was to scrutinize the FasL and Fasproteins by immunohistochemistry, FasL mRNA by RT-PCR and the apoptotic degrees by TUNEL analysis in theuterotubal junction (UTJ), isthmus, ampulla and infundibulum of swamp buffalo oviducts at follicular (n=5) and midluteal(n=5) phases. The …
Estrogen Receptor Alpha Localization In Thai Swamp Buffalo Oviduct During The Follicular And Luteal Phases, Paisan Tienthai, Kriengyot Sajjarengpong, Mongkol Techakumphu
Estrogen Receptor Alpha Localization In Thai Swamp Buffalo Oviduct During The Follicular And Luteal Phases, Paisan Tienthai, Kriengyot Sajjarengpong, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
Estrogens are known to be an important modulator for regulation of the reproductive function in female mammals. The specific receptor of estrogens in the buffalo reproductive tract, particularly in the oviduct, has not been explored yet in the expression pattern. This study was, therefore, undertaken to investigate the localization of the estrogen receptor alpha (ERα) in the oviduct of Thai swamp buffalo at the follicular and late luteal phases of the estrous cycle. The oviducts were collected from swamp buffalo genital tracts at the local abattoir and separated into the uterotubal junction (UTJ), isthmus, ampulla and infundibulum. Tissue distribution of …
Follicular Dynamics Following Estrus Synchronization In Swamp Buffalo Cows (Bubalus Bubalis), Akachart Promdireg, Giorgio Antonio Presicce, Fabio De Rensis, Jinda Singlor, Mongkol Techakumphu
Follicular Dynamics Following Estrus Synchronization In Swamp Buffalo Cows (Bubalus Bubalis), Akachart Promdireg, Giorgio Antonio Presicce, Fabio De Rensis, Jinda Singlor, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
The objective of this study was to elucidate ovarian follicular dynamics in swamp buffalo cows (Bubalus bulalis) following a protocol for estrus synchronization during hot as low breeding season (March to June) and cool as high breeding season (November to February). Nine pluriparous buffalo cows received a progesterone ear implant for 10 days together with the administration of a luteolytic dose of PGF2α at the time of implant removal to start the estrus cycle. Human Chorionic Gonadotrophin was administered whenever ovulation did not occur within the first five days following implant removal. Daily ultrasound monitoring and blood collection for progesterone …
The Determination Of Plasma Norepinephrine And Epinephrine In Dairy Cows And Swamp Buffaloes (Bubalus Bubalis) Using High Performance Liquid Chromatography With Electrochemical Detection, Sarinee Kalandakanond, Siripen Komolvanich, Sutthasinee Poonyachoti, Chollada Buranakarl
The Determination Of Plasma Norepinephrine And Epinephrine In Dairy Cows And Swamp Buffaloes (Bubalus Bubalis) Using High Performance Liquid Chromatography With Electrochemical Detection, Sarinee Kalandakanond, Siripen Komolvanich, Sutthasinee Poonyachoti, Chollada Buranakarl
The Thai Journal of Veterinary Medicine
In the present study, we report on the level of norepinephrine and epinephrine in healthy cattle, using high-performance liquid chromatography with an electrochemical detector (HPLC-EC). Sixteen crossbred Holstein dairy cows (> 87.5% Holstein) and fifteen swamp buffaloes (Bubalus bubalis), which included both sexes, were included. In the dairy cows, the plasma norepinephrine and epinephrine concentrations (mean ± SE) were 3.89 ± 0.51 nM and 0.82 + 0.12 nM, respectively. The concentrations of plasma norepinephrine and epinephrine in buffaloes (mean ± SE) were 3.75 ± 0.51 and 1.82 ± 0.45 nM, respectively.
An Electron Microscopic Study From Blood Cells And Platelets Of Swamp Buffaloes (Bubalus Bubalis), Wimon Pothiwong, Siripen Vethchagarun, Kriengyot Sajjarengpong, Adisorn Adirekthaworn
An Electron Microscopic Study From Blood Cells And Platelets Of Swamp Buffaloes (Bubalus Bubalis), Wimon Pothiwong, Siripen Vethchagarun, Kriengyot Sajjarengpong, Adisorn Adirekthaworn
The Thai Journal of Veterinary Medicine
Blood cells and platelets from five swamp buffaloes, three males and two females, were studied by electron microscope. The blood cells could be categorized into red blood cells, white blood cells and platelets. The red blood cells were round and had neither nucleus nor organelles. The white blood cells were divided into two groups; one with granules and the other without granules. The granulocytes (neutrophil, eosinophil and basophil) were round or oval and their nuclei were lobulated. The cytoplasm contained free ribosomes, a few mitochondria and endoplasmic reticulum. Specific granules of various sizes were distributed in the cytoplasm and the …
Embryo And Oocyte Collection In Thai Swamp Buffalo (Bubalus Bubalis) After Superovulation, Mongkol Techakumphu, Yant Sukwong, Malee Apimeteetumrong, Siriwat Intaramongkol, Jintana Intaramongkol
Embryo And Oocyte Collection In Thai Swamp Buffalo (Bubalus Bubalis) After Superovulation, Mongkol Techakumphu, Yant Sukwong, Malee Apimeteetumrong, Siriwat Intaramongkol, Jintana Intaramongkol
The Thai Journal of Veterinary Medicine
Six-day embryos were recovered from 25 swamp buffalo cows treated with pregnant mare serum gonadotropin (2,500 iu PMSG, n=9) and follicle stimulating hormone (280 mg, n=12 and 400 mg FSH, n=4). Two techniques, non surgical collection from live animals and direct flushing after slaughter were compared. The oocytes were collected from ovaries immediately after slaughter by aspiration with or without ovarian chopping. The studies showed that 0.44+0.59 embryos were collected from PMSG group, while 1.38+1.31 embryos were collected in FSH group. Fifty percent to 60% of recovered embryos were transferable. The non surgical technique gave a low recovery rate (14.40%) …
The Development Of Embryo Technogies For Swam Buffalo Reproduction In Thailand, Mongkol Techakumphu
The Development Of Embryo Technogies For Swam Buffalo Reproduction In Thailand, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
The objectives of embryo technologies development is to conserve the high genetic value swamp buffalo. Various fields of researches such as superovulation by gonadotropin hormone stimulation,. preimplantation embryonic development, embryo collection and transfer techniques, induction of twinning, cryopreservation of swamp buffalo embryos, follicular stimulation in prepubertal swamp buffalo calves and in vitro fertilization were well developped. Future studies in vitrification, embryo bank establishment, ovum pick up by ultrasounography and cloning technology are the field of interest in this species.